Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern Argentina
To study thraustochytrids from temperate and cold environments of Southern Argentina, the standard cultivation methodologies have been modified because many of the microorganisms detected by microscopic examination in both the original samples and the colonized baits failed to be successfully isolat...
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2011
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13403540_v52_n4_p242_Rosa http://hdl.handle.net/20.500.12110/paper_13403540_v52_n4_p242_Rosa |
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paper:paper_13403540_v52_n4_p242_Rosa2023-06-08T16:10:27Z Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern Argentina Rosa, Silvina Mariana Galvagno, Miguel Angel Vélez, Carlos Guillermo Alternative isolation procedures Elusive taxa Media formulation Thraustochytriales Water activity Labyrinthulida Thraustochytriidae Zea mays To study thraustochytrids from temperate and cold environments of Southern Argentina, the standard cultivation methodologies have been modified because many of the microorganisms detected by microscopic examination in both the original samples and the colonized baits failed to be successfully isolated in standard culture media. As a result, 35 strains, most of them having a very low growth rate, were isolated. Alternative procedures are proposed according to the nature of the sample, the characteristics of the thraustochytrid to be isolated, and the presence of contaminating microorganisms. Modifications proposed include the use of a newly formulated culture medium (Mar Chiquita, containing glucose, gelatine hydrolysate, peptone, and corn steep liquor as main carbon and nitrogen sources). In addition, the effects of the nutrient composition and agar concentration of culture media on the relative growth rates of the isolates were studied in an attempt to determine the most suitable conditions for the cultivation of new strains of thraustochytrids. The goal of this study is to develop a standard methodology, allowing us to grow baitable "elusive" thraustochytrid strains, and that could be applied to improve the isolation and the study of the undocumented biodiversity of this group of microorganisms from different environments. © 2010 The Mycological Society of Japan and Springer. Fil:Rosa, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Galvagno, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vélez, C.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13403540_v52_n4_p242_Rosa http://hdl.handle.net/20.500.12110/paper_13403540_v52_n4_p242_Rosa |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Alternative isolation procedures Elusive taxa Media formulation Thraustochytriales Water activity Labyrinthulida Thraustochytriidae Zea mays |
| spellingShingle |
Alternative isolation procedures Elusive taxa Media formulation Thraustochytriales Water activity Labyrinthulida Thraustochytriidae Zea mays Rosa, Silvina Mariana Galvagno, Miguel Angel Vélez, Carlos Guillermo Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern Argentina |
| topic_facet |
Alternative isolation procedures Elusive taxa Media formulation Thraustochytriales Water activity Labyrinthulida Thraustochytriidae Zea mays |
| description |
To study thraustochytrids from temperate and cold environments of Southern Argentina, the standard cultivation methodologies have been modified because many of the microorganisms detected by microscopic examination in both the original samples and the colonized baits failed to be successfully isolated in standard culture media. As a result, 35 strains, most of them having a very low growth rate, were isolated. Alternative procedures are proposed according to the nature of the sample, the characteristics of the thraustochytrid to be isolated, and the presence of contaminating microorganisms. Modifications proposed include the use of a newly formulated culture medium (Mar Chiquita, containing glucose, gelatine hydrolysate, peptone, and corn steep liquor as main carbon and nitrogen sources). In addition, the effects of the nutrient composition and agar concentration of culture media on the relative growth rates of the isolates were studied in an attempt to determine the most suitable conditions for the cultivation of new strains of thraustochytrids. The goal of this study is to develop a standard methodology, allowing us to grow baitable "elusive" thraustochytrid strains, and that could be applied to improve the isolation and the study of the undocumented biodiversity of this group of microorganisms from different environments. © 2010 The Mycological Society of Japan and Springer. |
| author |
Rosa, Silvina Mariana Galvagno, Miguel Angel Vélez, Carlos Guillermo |
| author_facet |
Rosa, Silvina Mariana Galvagno, Miguel Angel Vélez, Carlos Guillermo |
| author_sort |
Rosa, Silvina Mariana |
| title |
Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern Argentina |
| title_short |
Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern Argentina |
| title_full |
Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern Argentina |
| title_fullStr |
Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern Argentina |
| title_full_unstemmed |
Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern Argentina |
| title_sort |
adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern argentina |
| publishDate |
2011 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13403540_v52_n4_p242_Rosa http://hdl.handle.net/20.500.12110/paper_13403540_v52_n4_p242_Rosa |
| work_keys_str_mv |
AT rosasilvinamariana adjustingcultureconditionstoisolatethraustochytridsfromtemperateandcoldenvironmentsinsouthernargentina AT galvagnomiguelangel adjustingcultureconditionstoisolatethraustochytridsfromtemperateandcoldenvironmentsinsouthernargentina AT velezcarlosguillermo adjustingcultureconditionstoisolatethraustochytridsfromtemperateandcoldenvironmentsinsouthernargentina |
| _version_ |
1768545476838162432 |