Hepatic 11 beta-hydroxysteroid dehydrogenase 1 involvement in alterations of glucose metabolism produced by acidotic stress in rat

11 beta-hydroxysteroid dehydrogenase (HSDs) enzymes regulate the activity of glucocorticoids in target organs. HSD1, one of the two existing isoforms, locates mainly in CNS, liver and adipose tissue. HSD1 is involved in the pathogenesis of diseases such as obesity, insulin resistance, arterial hyper...

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Autor principal:	Mazzetti, Marta Blanca
Publicado:	2009
Materias:	Glucose Glycyrrhetinic acid HSD1 Pepck Stress 11beta hydroxysteroid dehydrogenase 1 carbohydrate chloride glucose glycogen phosphorylase glycyrrhetinic acid phosphoenolpyruvate carboxykinase (ATP) acidity animal experiment animal tissue article carbohydrate metabolism controlled study enzyme activity glucose blood level glucose metabolism male metabolic syndrome X non insulin dependent diabetes mellitus nonhuman rat 11-beta-Hydroxysteroid Dehydrogenases Adipose Tissue Animals Carbohydrate Metabolism Carbohydrates Central Nervous System Glycyrrhetinic Acid Liver Male Models, Biological Phosphoenolpyruvate Carboxykinase (ATP) Protein Isoforms Rats Rats, Sprague-Dawley Animalia Rattus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_11387548_v65_n4_p329_Altuna http://hdl.handle.net/20.500.12110/paper_11387548_v65_n4_p329_Altuna
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_11387548_v65_n4_p329_Altuna
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spelling	paper:paper_11387548_v65_n4_p329_Altuna2023-06-08T16:09:12Z Hepatic 11 beta-hydroxysteroid dehydrogenase 1 involvement in alterations of glucose metabolism produced by acidotic stress in rat Mazzetti, Marta Blanca Glucose Glycyrrhetinic acid HSD1 Pepck Stress 11beta hydroxysteroid dehydrogenase 1 carbohydrate chloride glucose glycogen phosphorylase glycyrrhetinic acid phosphoenolpyruvate carboxykinase (ATP) acidity animal experiment animal tissue article carbohydrate metabolism controlled study enzyme activity glucose blood level glucose metabolism male metabolic syndrome X non insulin dependent diabetes mellitus nonhuman rat 11-beta-Hydroxysteroid Dehydrogenases Adipose Tissue Animals Carbohydrate Metabolism Carbohydrates Central Nervous System Glucose Glycyrrhetinic Acid Liver Male Models, Biological Phosphoenolpyruvate Carboxykinase (ATP) Protein Isoforms Rats Rats, Sprague-Dawley Animalia Rattus 11 beta-hydroxysteroid dehydrogenase (HSDs) enzymes regulate the activity of glucocorticoids in target organs. HSD1, one of the two existing isoforms, locates mainly in CNS, liver and adipose tissue. HSD1 is involved in the pathogenesis of diseases such as obesity, insulin resistance, arterial hypertension and the Metabolic Syndrome. The stress produced by HC1 overload triggers metabolic acidosis and increases liver HSD1 activity associated with increased phosphoenolpyruvate carboxykinase, a regulatory enzyme of gluconeogenesis that is activated by glucocorticoids, with increased glycaemia and glycogen breakdown. The aim of this study was to analyze whether the metabolic modifications triggered by HC1 stress are due to increased liver HSD1 activity. Glycyrrhetinic acid, a potent HDS inhibitor, was administered subcutaneously (20 mg/ml) to stressed and unstressed four months old male Sprague Dawley rats to investigate changes in liver HSD1, phosphoenolpyruvate carboxykinase (PECPK) and glycogen phosphorylase activities and plasma glucose levels. It was observed that all these parameters increased in stressed animals, but that treatment with glycyrrhetinic acid significantly reduced their levels. In conclusion, our results demonstrate the involvement of HSD1 in stress induced carbohydrate disturbances and could contribute to the impact of HSD1 inhibitors on carbohydrate metabolism and its relevance in the study of Metabolic Syndrome Disorder and non insulin-dependent diabetes mellitus. Fil:Mazzetti, M.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_11387548_v65_n4_p329_Altuna http://hdl.handle.net/20.500.12110/paper_11387548_v65_n4_p329_Altuna
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Glucose Glycyrrhetinic acid HSD1 Pepck Stress 11beta hydroxysteroid dehydrogenase 1 carbohydrate chloride glucose glycogen phosphorylase glycyrrhetinic acid phosphoenolpyruvate carboxykinase (ATP) acidity animal experiment animal tissue article carbohydrate metabolism controlled study enzyme activity glucose blood level glucose metabolism male metabolic syndrome X non insulin dependent diabetes mellitus nonhuman rat 11-beta-Hydroxysteroid Dehydrogenases Adipose Tissue Animals Carbohydrate Metabolism Carbohydrates Central Nervous System Glucose Glycyrrhetinic Acid Liver Male Models, Biological Phosphoenolpyruvate Carboxykinase (ATP) Protein Isoforms Rats Rats, Sprague-Dawley Animalia Rattus
spellingShingle	Glucose Glycyrrhetinic acid HSD1 Pepck Stress 11beta hydroxysteroid dehydrogenase 1 carbohydrate chloride glucose glycogen phosphorylase glycyrrhetinic acid phosphoenolpyruvate carboxykinase (ATP) acidity animal experiment animal tissue article carbohydrate metabolism controlled study enzyme activity glucose blood level glucose metabolism male metabolic syndrome X non insulin dependent diabetes mellitus nonhuman rat 11-beta-Hydroxysteroid Dehydrogenases Adipose Tissue Animals Carbohydrate Metabolism Carbohydrates Central Nervous System Glucose Glycyrrhetinic Acid Liver Male Models, Biological Phosphoenolpyruvate Carboxykinase (ATP) Protein Isoforms Rats Rats, Sprague-Dawley Animalia Rattus Mazzetti, Marta Blanca Hepatic 11 beta-hydroxysteroid dehydrogenase 1 involvement in alterations of glucose metabolism produced by acidotic stress in rat
topic_facet	Glucose Glycyrrhetinic acid HSD1 Pepck Stress 11beta hydroxysteroid dehydrogenase 1 carbohydrate chloride glucose glycogen phosphorylase glycyrrhetinic acid phosphoenolpyruvate carboxykinase (ATP) acidity animal experiment animal tissue article carbohydrate metabolism controlled study enzyme activity glucose blood level glucose metabolism male metabolic syndrome X non insulin dependent diabetes mellitus nonhuman rat 11-beta-Hydroxysteroid Dehydrogenases Adipose Tissue Animals Carbohydrate Metabolism Carbohydrates Central Nervous System Glucose Glycyrrhetinic Acid Liver Male Models, Biological Phosphoenolpyruvate Carboxykinase (ATP) Protein Isoforms Rats Rats, Sprague-Dawley Animalia Rattus
description	11 beta-hydroxysteroid dehydrogenase (HSDs) enzymes regulate the activity of glucocorticoids in target organs. HSD1, one of the two existing isoforms, locates mainly in CNS, liver and adipose tissue. HSD1 is involved in the pathogenesis of diseases such as obesity, insulin resistance, arterial hypertension and the Metabolic Syndrome. The stress produced by HC1 overload triggers metabolic acidosis and increases liver HSD1 activity associated with increased phosphoenolpyruvate carboxykinase, a regulatory enzyme of gluconeogenesis that is activated by glucocorticoids, with increased glycaemia and glycogen breakdown. The aim of this study was to analyze whether the metabolic modifications triggered by HC1 stress are due to increased liver HSD1 activity. Glycyrrhetinic acid, a potent HDS inhibitor, was administered subcutaneously (20 mg/ml) to stressed and unstressed four months old male Sprague Dawley rats to investigate changes in liver HSD1, phosphoenolpyruvate carboxykinase (PECPK) and glycogen phosphorylase activities and plasma glucose levels. It was observed that all these parameters increased in stressed animals, but that treatment with glycyrrhetinic acid significantly reduced their levels. In conclusion, our results demonstrate the involvement of HSD1 in stress induced carbohydrate disturbances and could contribute to the impact of HSD1 inhibitors on carbohydrate metabolism and its relevance in the study of Metabolic Syndrome Disorder and non insulin-dependent diabetes mellitus.
author	Mazzetti, Marta Blanca
author_facet	Mazzetti, Marta Blanca
author_sort	Mazzetti, Marta Blanca
title	Hepatic 11 beta-hydroxysteroid dehydrogenase 1 involvement in alterations of glucose metabolism produced by acidotic stress in rat
title_short	Hepatic 11 beta-hydroxysteroid dehydrogenase 1 involvement in alterations of glucose metabolism produced by acidotic stress in rat
title_full	Hepatic 11 beta-hydroxysteroid dehydrogenase 1 involvement in alterations of glucose metabolism produced by acidotic stress in rat
title_fullStr	Hepatic 11 beta-hydroxysteroid dehydrogenase 1 involvement in alterations of glucose metabolism produced by acidotic stress in rat
title_full_unstemmed	Hepatic 11 beta-hydroxysteroid dehydrogenase 1 involvement in alterations of glucose metabolism produced by acidotic stress in rat
title_sort	hepatic 11 beta-hydroxysteroid dehydrogenase 1 involvement in alterations of glucose metabolism produced by acidotic stress in rat
publishDate	2009
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_11387548_v65_n4_p329_Altuna http://hdl.handle.net/20.500.12110/paper_11387548_v65_n4_p329_Altuna
work_keys_str_mv	AT mazzettimartablanca hepatic11betahydroxysteroiddehydrogenase1involvementinalterationsofglucosemetabolismproducedbyacidoticstressinrat
_version_	1768543480579096576

Hepatic 11 beta-hydroxysteroid dehydrogenase 1 involvement in alterations of glucose metabolism produced by acidotic stress in rat

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