Differential Erythropoietin Action upon Cells Induced to Eryptosis by Different Agents

Eryptosis is a process by which mature erythrocytes can undergo self-destruction sharing several features with apoptosis. Premature programmed erythrocyte death may be induced by different agents. In this study, we compared mechanisms involved in two eryptotic models (oxidative stress and cell calci...

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Autores principales:	Vota, Daiana Marina, Wenker, Shirley Denise, Nesse, Alcira Beatriz, Vittori, Daniela Cecilia
Publicado:	2013
Materias:	Calcium overload Eryptosis Erythropoietin Oxidative stress calcimycin calcium erythropoietin hydrogen peroxide ionophore oxidizing agent phosphatidylserine reactive oxygen metabolite sodium nitrite adult apoptosis article cell culture cytology dose response drug effect erythrocyte exocytosis flow cytometry human intracellular space lipid peroxidation metabolism oxidative stress Adult Apoptosis Calcimycin Calcium Cells, Cultured Dose-Response Relationship, Drug Erythrocytes Exocytosis Flow Cytometry Humans Hydrogen Peroxide Intracellular Space Ionophores Lipid Peroxidation Oxidants Oxidative Stress Phosphatidylserines Reactive Oxygen Species Sodium Nitrite
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10859195_v65_n2_p145_Vota http://hdl.handle.net/20.500.12110/paper_10859195_v65_n2_p145_Vota
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_10859195_v65_n2_p145_Vota
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spelling	paper:paper_10859195_v65_n2_p145_Vota2023-06-08T16:06:09Z Differential Erythropoietin Action upon Cells Induced to Eryptosis by Different Agents Vota, Daiana Marina Wenker, Shirley Denise Nesse, Alcira Beatriz Vittori, Daniela Cecilia Calcium overload Eryptosis Erythropoietin Oxidative stress calcimycin calcium erythropoietin hydrogen peroxide ionophore oxidizing agent phosphatidylserine reactive oxygen metabolite sodium nitrite adult apoptosis article cell culture cytology dose response drug effect erythrocyte exocytosis flow cytometry human intracellular space lipid peroxidation metabolism oxidative stress Adult Apoptosis Calcimycin Calcium Cells, Cultured Dose-Response Relationship, Drug Erythrocytes Erythropoietin Exocytosis Flow Cytometry Humans Hydrogen Peroxide Intracellular Space Ionophores Lipid Peroxidation Oxidants Oxidative Stress Phosphatidylserines Reactive Oxygen Species Sodium Nitrite Eryptosis is a process by which mature erythrocytes can undergo self-destruction sharing several features with apoptosis. Premature programmed erythrocyte death may be induced by different agents. In this study, we compared mechanisms involved in two eryptotic models (oxidative stress and cell calcium overload) so as to distinguish whether they share signaling pathways and could be prevented by erythropoietin (Epo). Phosphatidylserine (PS) translocation and increased calcium content were common signs in erythrocytes exposed to sodium nitrite plus hydrogen peroxide or calcium ionophore A23187 (CaI), while increased ROS and decreased GSH levels were detected in the oxidative model. Protein kinase activation seemed to be an outstanding feature in eryptosis induced by oxidative stress, whereas phosphatase activation was favored in the CaI model. Cell morphology and membrane protein modifications were also differential signs between both models. Epo was able to prevent cell oxidative imbalance, thus blunting PS translocation. However, the hormone favored intracellular calcium influx which could be the reason why it could not completely counteract the induction of eryptosis. Instead, Epo was unable to inhibit PS externalization in the CaI model. The different mechanisms involved in the eryptotic models may explain the differential action of Epo upon erythrocytes induced to eryptosis by different agents. © 2012 Springer Science+Business Media, LLC. Fil:Vota, D.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wenker, S.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Nesse, A.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vittori, D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10859195_v65_n2_p145_Vota http://hdl.handle.net/20.500.12110/paper_10859195_v65_n2_p145_Vota
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Calcium overload Eryptosis Erythropoietin Oxidative stress calcimycin calcium erythropoietin hydrogen peroxide ionophore oxidizing agent phosphatidylserine reactive oxygen metabolite sodium nitrite adult apoptosis article cell culture cytology dose response drug effect erythrocyte exocytosis flow cytometry human intracellular space lipid peroxidation metabolism oxidative stress Adult Apoptosis Calcimycin Calcium Cells, Cultured Dose-Response Relationship, Drug Erythrocytes Erythropoietin Exocytosis Flow Cytometry Humans Hydrogen Peroxide Intracellular Space Ionophores Lipid Peroxidation Oxidants Oxidative Stress Phosphatidylserines Reactive Oxygen Species Sodium Nitrite
spellingShingle	Calcium overload Eryptosis Erythropoietin Oxidative stress calcimycin calcium erythropoietin hydrogen peroxide ionophore oxidizing agent phosphatidylserine reactive oxygen metabolite sodium nitrite adult apoptosis article cell culture cytology dose response drug effect erythrocyte exocytosis flow cytometry human intracellular space lipid peroxidation metabolism oxidative stress Adult Apoptosis Calcimycin Calcium Cells, Cultured Dose-Response Relationship, Drug Erythrocytes Erythropoietin Exocytosis Flow Cytometry Humans Hydrogen Peroxide Intracellular Space Ionophores Lipid Peroxidation Oxidants Oxidative Stress Phosphatidylserines Reactive Oxygen Species Sodium Nitrite Vota, Daiana Marina Wenker, Shirley Denise Nesse, Alcira Beatriz Vittori, Daniela Cecilia Differential Erythropoietin Action upon Cells Induced to Eryptosis by Different Agents
topic_facet	Calcium overload Eryptosis Erythropoietin Oxidative stress calcimycin calcium erythropoietin hydrogen peroxide ionophore oxidizing agent phosphatidylserine reactive oxygen metabolite sodium nitrite adult apoptosis article cell culture cytology dose response drug effect erythrocyte exocytosis flow cytometry human intracellular space lipid peroxidation metabolism oxidative stress Adult Apoptosis Calcimycin Calcium Cells, Cultured Dose-Response Relationship, Drug Erythrocytes Erythropoietin Exocytosis Flow Cytometry Humans Hydrogen Peroxide Intracellular Space Ionophores Lipid Peroxidation Oxidants Oxidative Stress Phosphatidylserines Reactive Oxygen Species Sodium Nitrite
description	Eryptosis is a process by which mature erythrocytes can undergo self-destruction sharing several features with apoptosis. Premature programmed erythrocyte death may be induced by different agents. In this study, we compared mechanisms involved in two eryptotic models (oxidative stress and cell calcium overload) so as to distinguish whether they share signaling pathways and could be prevented by erythropoietin (Epo). Phosphatidylserine (PS) translocation and increased calcium content were common signs in erythrocytes exposed to sodium nitrite plus hydrogen peroxide or calcium ionophore A23187 (CaI), while increased ROS and decreased GSH levels were detected in the oxidative model. Protein kinase activation seemed to be an outstanding feature in eryptosis induced by oxidative stress, whereas phosphatase activation was favored in the CaI model. Cell morphology and membrane protein modifications were also differential signs between both models. Epo was able to prevent cell oxidative imbalance, thus blunting PS translocation. However, the hormone favored intracellular calcium influx which could be the reason why it could not completely counteract the induction of eryptosis. Instead, Epo was unable to inhibit PS externalization in the CaI model. The different mechanisms involved in the eryptotic models may explain the differential action of Epo upon erythrocytes induced to eryptosis by different agents. © 2012 Springer Science+Business Media, LLC.
author	Vota, Daiana Marina Wenker, Shirley Denise Nesse, Alcira Beatriz Vittori, Daniela Cecilia
author_facet	Vota, Daiana Marina Wenker, Shirley Denise Nesse, Alcira Beatriz Vittori, Daniela Cecilia
author_sort	Vota, Daiana Marina
title	Differential Erythropoietin Action upon Cells Induced to Eryptosis by Different Agents
title_short	Differential Erythropoietin Action upon Cells Induced to Eryptosis by Different Agents
title_full	Differential Erythropoietin Action upon Cells Induced to Eryptosis by Different Agents
title_fullStr	Differential Erythropoietin Action upon Cells Induced to Eryptosis by Different Agents
title_full_unstemmed	Differential Erythropoietin Action upon Cells Induced to Eryptosis by Different Agents
title_sort	differential erythropoietin action upon cells induced to eryptosis by different agents
publishDate	2013
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10859195_v65_n2_p145_Vota http://hdl.handle.net/20.500.12110/paper_10859195_v65_n2_p145_Vota
work_keys_str_mv	AT votadaianamarina differentialerythropoietinactionuponcellsinducedtoeryptosisbydifferentagents AT wenkershirleydenise differentialerythropoietinactionuponcellsinducedtoeryptosisbydifferentagents AT nessealcirabeatriz differentialerythropoietinactionuponcellsinducedtoeryptosisbydifferentagents AT vittoridanielacecilia differentialerythropoietinactionuponcellsinducedtoeryptosisbydifferentagents
_version_	1768542047659098112

Differential Erythropoietin Action upon Cells Induced to Eryptosis by Different Agents

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