Exchange of microtubule molecular motors during melanosome transport in Xenopus laevis melanophores is triggered by collisions with intracellular obstacles
The observation that several cargoes move bidirectionally along microtubules in vivo raised the question regarding how molecular motors with opposed polarity coordinate during transport. In this work, we analyzed the switch of microtubule motors during the transport of melanosomes in Xenopus melanop...
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2008
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10859195_v52_n3_p191_Bruno http://hdl.handle.net/20.500.12110/paper_10859195_v52_n3_p191_Bruno |
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paper:paper_10859195_v52_n3_p191_Bruno2023-06-08T16:06:08Z Exchange of microtubule molecular motors during melanosome transport in Xenopus laevis melanophores is triggered by collisions with intracellular obstacles Bruno, Luciana Levi, Valeria Coordination Melanosome transport Microtubule molecular motors Xenopus melanophores Xenopus laevis algorithm animal article cell culture chemistry melanophore melanosome metabolism microtubule physiology statistical model time transport at the cellular level Xenopus laevis Algorithms Animals Biological Transport Cells, Cultured Melanophores Melanosomes Microtubules Models, Statistical Time Factors Xenopus laevis The observation that several cargoes move bidirectionally along microtubules in vivo raised the question regarding how molecular motors with opposed polarity coordinate during transport. In this work, we analyzed the switch of microtubule motors during the transport of melanosomes in Xenopus melanophores by registering trajectories of these organelles moving along microtubules using a fast and precise tracking method. We analyzed in detail the intervals of trajectories showing reversions in the original direction of transport and processive motion in the opposite direction for at least 250 nm. In most of the cases, the speed of the melanosome before the reversion slowly decreases with time approaching zero then, the organelle returns over the same path moving initially at a very high speed and slowing down with time. These results could be explained according to a model in which reversions are triggered by an elastic collision of the cargo with obstacles in the cytosol. This interaction generates a force opposed to the movement of the motor-driven organelle increasing the probability of detaching the active motors from the track. The model can explain reversions in melanosome trajectories as well as other characteristics of in vivo transport along microtubules observed by other authors. Our results suggest that the crowded cytoplasm plays a key role in regulating the coordination of microtubules-dependent motors. © 2008 Humana Press Inc. Fil:Bruno, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Levi, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10859195_v52_n3_p191_Bruno http://hdl.handle.net/20.500.12110/paper_10859195_v52_n3_p191_Bruno |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Coordination Melanosome transport Microtubule molecular motors Xenopus melanophores Xenopus laevis algorithm animal article cell culture chemistry melanophore melanosome metabolism microtubule physiology statistical model time transport at the cellular level Xenopus laevis Algorithms Animals Biological Transport Cells, Cultured Melanophores Melanosomes Microtubules Models, Statistical Time Factors Xenopus laevis |
| spellingShingle |
Coordination Melanosome transport Microtubule molecular motors Xenopus melanophores Xenopus laevis algorithm animal article cell culture chemistry melanophore melanosome metabolism microtubule physiology statistical model time transport at the cellular level Xenopus laevis Algorithms Animals Biological Transport Cells, Cultured Melanophores Melanosomes Microtubules Models, Statistical Time Factors Xenopus laevis Bruno, Luciana Levi, Valeria Exchange of microtubule molecular motors during melanosome transport in Xenopus laevis melanophores is triggered by collisions with intracellular obstacles |
| topic_facet |
Coordination Melanosome transport Microtubule molecular motors Xenopus melanophores Xenopus laevis algorithm animal article cell culture chemistry melanophore melanosome metabolism microtubule physiology statistical model time transport at the cellular level Xenopus laevis Algorithms Animals Biological Transport Cells, Cultured Melanophores Melanosomes Microtubules Models, Statistical Time Factors Xenopus laevis |
| description |
The observation that several cargoes move bidirectionally along microtubules in vivo raised the question regarding how molecular motors with opposed polarity coordinate during transport. In this work, we analyzed the switch of microtubule motors during the transport of melanosomes in Xenopus melanophores by registering trajectories of these organelles moving along microtubules using a fast and precise tracking method. We analyzed in detail the intervals of trajectories showing reversions in the original direction of transport and processive motion in the opposite direction for at least 250 nm. In most of the cases, the speed of the melanosome before the reversion slowly decreases with time approaching zero then, the organelle returns over the same path moving initially at a very high speed and slowing down with time. These results could be explained according to a model in which reversions are triggered by an elastic collision of the cargo with obstacles in the cytosol. This interaction generates a force opposed to the movement of the motor-driven organelle increasing the probability of detaching the active motors from the track. The model can explain reversions in melanosome trajectories as well as other characteristics of in vivo transport along microtubules observed by other authors. Our results suggest that the crowded cytoplasm plays a key role in regulating the coordination of microtubules-dependent motors. © 2008 Humana Press Inc. |
| author |
Bruno, Luciana Levi, Valeria |
| author_facet |
Bruno, Luciana Levi, Valeria |
| author_sort |
Bruno, Luciana |
| title |
Exchange of microtubule molecular motors during melanosome transport in Xenopus laevis melanophores is triggered by collisions with intracellular obstacles |
| title_short |
Exchange of microtubule molecular motors during melanosome transport in Xenopus laevis melanophores is triggered by collisions with intracellular obstacles |
| title_full |
Exchange of microtubule molecular motors during melanosome transport in Xenopus laevis melanophores is triggered by collisions with intracellular obstacles |
| title_fullStr |
Exchange of microtubule molecular motors during melanosome transport in Xenopus laevis melanophores is triggered by collisions with intracellular obstacles |
| title_full_unstemmed |
Exchange of microtubule molecular motors during melanosome transport in Xenopus laevis melanophores is triggered by collisions with intracellular obstacles |
| title_sort |
exchange of microtubule molecular motors during melanosome transport in xenopus laevis melanophores is triggered by collisions with intracellular obstacles |
| publishDate |
2008 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10859195_v52_n3_p191_Bruno http://hdl.handle.net/20.500.12110/paper_10859195_v52_n3_p191_Bruno |
| work_keys_str_mv |
AT brunoluciana exchangeofmicrotubulemolecularmotorsduringmelanosometransportinxenopuslaevismelanophoresistriggeredbycollisionswithintracellularobstacles AT levivaleria exchangeofmicrotubulemolecularmotorsduringmelanosometransportinxenopuslaevismelanophoresistriggeredbycollisionswithintracellularobstacles |
| _version_ |
1768542852697030656 |