Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition
Rosmarinus officinalis extracts were investigated by a combination of bioassays and biochemical analysis to identify bioactive compounds. The 2,2-diphenyl-2-picrylhydracyl hydrate (DPPH) radical scavenging method, Folin-Ciocaulteau method and HPLC chromatography were used to study the distribution a...
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paper:paper_10715762_v40_n2_p223_Moreno2023-06-08T16:04:44Z Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition Moreno de Colonna, Silvia Vojnov, Adrian Alberto Antimicrobial activity Antioxidants Carnosic acid Rosmarinic acid Rosmarinus officinalis carnosic acid carnosol rosmarinic acid Rosmarinus officinalis extract scavenger antimicrobial activity antioxidant activity article chemical composition controlled study correlation analysis drug mechanism drug screening drug synthesis high performance liquid chromatography minimum inhibitory concentration nonhuman rosemary statistical analysis statistical significance Anti-Infective Agents Antioxidants Bacteria Chromatography, High Pressure Liquid Cinnamates Depsides Diterpenes, Abietane Flavonoids Free Radicals Indicators and Reagents Phenanthrenes Phenols Picrates Plant Extracts Rosmarinus Saccharomyces cerevisiae Negibacteria Posibacteria Rosmarinus officinalis Rosmarinus officinalis extracts were investigated by a combination of bioassays and biochemical analysis to identify bioactive compounds. The 2,2-diphenyl-2-picrylhydracyl hydrate (DPPH) radical scavenging method, Folin-Ciocaulteau method and HPLC chromatography were used to study the distribution and levels of antioxidants (AOXs). Antimicrobial activity analysis was carried out using the disk diffusion and broth dilution techniques. A good correlation between the AOX activities and total phenol content in the extracts was found. Although all rosemary extracts showed a high radical scavenging activity, a different efficacy as antimicrobial agent was observed. Methanol extract containing 30% of carnosic acid, 16% of carnosol and 5% of rosmarinic acid was the most effective antimicrobial against Gram positive bacteria (minimal inhibition concentration, MIC, between 2 and 15 μg/ml), Gram negative bacteria (MIC between 2 and 60 μg/ml) and yeast (MIC of 4 μg/ml). By contrast, water extract containing only 15% of rosmarinic acid showed a narrow activity. MIC value of the methanol and water extracts is in a good correlation with the values obtained with pure carnosic acid and rosmarinic acid, respectively. Therefore, our results suggested that the antimicrobial rosemary extracts efficacy was associated with their specific phenolic composition. Carnosic acid and rosmarinic acid may be the main bioactive antimicrobial compounds present in rosemary extracts. From a practical point of view, rosemary extract may be a good candidate for functional foods as well as for pharmaceutical plant-based products. © 2006 Taylor & Francis. Fil:Moreno, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vojnov, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10715762_v40_n2_p223_Moreno http://hdl.handle.net/20.500.12110/paper_10715762_v40_n2_p223_Moreno |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antimicrobial activity Antioxidants Carnosic acid Rosmarinic acid Rosmarinus officinalis carnosic acid carnosol rosmarinic acid Rosmarinus officinalis extract scavenger antimicrobial activity antioxidant activity article chemical composition controlled study correlation analysis drug mechanism drug screening drug synthesis high performance liquid chromatography minimum inhibitory concentration nonhuman rosemary statistical analysis statistical significance Anti-Infective Agents Antioxidants Bacteria Chromatography, High Pressure Liquid Cinnamates Depsides Diterpenes, Abietane Flavonoids Free Radicals Indicators and Reagents Phenanthrenes Phenols Picrates Plant Extracts Rosmarinus Saccharomyces cerevisiae Negibacteria Posibacteria Rosmarinus officinalis |
| spellingShingle |
Antimicrobial activity Antioxidants Carnosic acid Rosmarinic acid Rosmarinus officinalis carnosic acid carnosol rosmarinic acid Rosmarinus officinalis extract scavenger antimicrobial activity antioxidant activity article chemical composition controlled study correlation analysis drug mechanism drug screening drug synthesis high performance liquid chromatography minimum inhibitory concentration nonhuman rosemary statistical analysis statistical significance Anti-Infective Agents Antioxidants Bacteria Chromatography, High Pressure Liquid Cinnamates Depsides Diterpenes, Abietane Flavonoids Free Radicals Indicators and Reagents Phenanthrenes Phenols Picrates Plant Extracts Rosmarinus Saccharomyces cerevisiae Negibacteria Posibacteria Rosmarinus officinalis Moreno de Colonna, Silvia Vojnov, Adrian Alberto Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition |
| topic_facet |
Antimicrobial activity Antioxidants Carnosic acid Rosmarinic acid Rosmarinus officinalis carnosic acid carnosol rosmarinic acid Rosmarinus officinalis extract scavenger antimicrobial activity antioxidant activity article chemical composition controlled study correlation analysis drug mechanism drug screening drug synthesis high performance liquid chromatography minimum inhibitory concentration nonhuman rosemary statistical analysis statistical significance Anti-Infective Agents Antioxidants Bacteria Chromatography, High Pressure Liquid Cinnamates Depsides Diterpenes, Abietane Flavonoids Free Radicals Indicators and Reagents Phenanthrenes Phenols Picrates Plant Extracts Rosmarinus Saccharomyces cerevisiae Negibacteria Posibacteria Rosmarinus officinalis |
| description |
Rosmarinus officinalis extracts were investigated by a combination of bioassays and biochemical analysis to identify bioactive compounds. The 2,2-diphenyl-2-picrylhydracyl hydrate (DPPH) radical scavenging method, Folin-Ciocaulteau method and HPLC chromatography were used to study the distribution and levels of antioxidants (AOXs). Antimicrobial activity analysis was carried out using the disk diffusion and broth dilution techniques. A good correlation between the AOX activities and total phenol content in the extracts was found. Although all rosemary extracts showed a high radical scavenging activity, a different efficacy as antimicrobial agent was observed. Methanol extract containing 30% of carnosic acid, 16% of carnosol and 5% of rosmarinic acid was the most effective antimicrobial against Gram positive bacteria (minimal inhibition concentration, MIC, between 2 and 15 μg/ml), Gram negative bacteria (MIC between 2 and 60 μg/ml) and yeast (MIC of 4 μg/ml). By contrast, water extract containing only 15% of rosmarinic acid showed a narrow activity. MIC value of the methanol and water extracts is in a good correlation with the values obtained with pure carnosic acid and rosmarinic acid, respectively. Therefore, our results suggested that the antimicrobial rosemary extracts efficacy was associated with their specific phenolic composition. Carnosic acid and rosmarinic acid may be the main bioactive antimicrobial compounds present in rosemary extracts. From a practical point of view, rosemary extract may be a good candidate for functional foods as well as for pharmaceutical plant-based products. © 2006 Taylor & Francis. |
| author |
Moreno de Colonna, Silvia Vojnov, Adrian Alberto |
| author_facet |
Moreno de Colonna, Silvia Vojnov, Adrian Alberto |
| author_sort |
Moreno de Colonna, Silvia |
| title |
Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition |
| title_short |
Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition |
| title_full |
Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition |
| title_fullStr |
Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition |
| title_full_unstemmed |
Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition |
| title_sort |
antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10715762_v40_n2_p223_Moreno http://hdl.handle.net/20.500.12110/paper_10715762_v40_n2_p223_Moreno |
| work_keys_str_mv |
AT morenodecolonnasilvia antioxidantandantimicrobialactivitiesofrosemaryextractslinkedtotheirpolyphenolcomposition AT vojnovadrianalberto antioxidantandantimicrobialactivitiesofrosemaryextractslinkedtotheirpolyphenolcomposition |
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