Adenyiyl Cyclase and G‐Proteins in Phytomonas
ABSTRACT. Phytomonas sp. membranes have an adenylyl cyclase activity which is greater in the presence of Mn2+ than with Mg2+. The Mg2+ and Mn2+ activity ratio varies from one membrane preparation to another, suggesting that the adenylyl cyclase has a variable activation state. A [35S]GTP‐γ‐S‐binding...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Publicado: |
1995
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10665234_v42_n3_p257_Farber http://hdl.handle.net/20.500.12110/paper_10665234_v42_n3_p257_Farber |
| Aporte de: |
| id |
paper:paper_10665234_v42_n3_p257_Farber |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_10665234_v42_n3_p257_Farber2023-06-08T16:04:16Z Adenyiyl Cyclase and G‐Proteins in Phytomonas Farber, Marisa Diana Paveto, María Cristina Torres, Héctor Norberto Flawiá, Mirtha María ADP‐ribosylation cholera toxin pertussis toxin plant trypanosomatids adenosine diphosphate adenylate cyclase adenylate cyclase toxin bacterial toxin Bordetella virulence factor cholera toxin divalent cation guanosine triphosphate heterotrimeric guanine nucleotide binding protein magnesium manganese nicotinamide adenine dinucleotide pertussis toxin animal article cell membrane cytology drug effect enzyme activation enzymology flagellate metabolism molecular weight protein binding Adenosine Diphosphate Adenylate Cyclase Adenylate Cyclase Toxin Animals Bacterial Toxins Cations, Divalent Cell Membrane Cholera Toxin Enzyme Activation Guanosine Triphosphate Heterotrimeric GTP-Binding Proteins Magnesium Manganese Molecular Weight NAD Pertussis Toxin Protein Binding Trypanosomatina Virulence Factors, Bordetella ABSTRACT. Phytomonas sp. membranes have an adenylyl cyclase activity which is greater in the presence of Mn2+ than with Mg2+. The Mg2+ and Mn2+ activity ratio varies from one membrane preparation to another, suggesting that the adenylyl cyclase has a variable activation state. A [35S]GTP‐γ‐S‐binding activity with a Kd of 171 nM was detected in Phytomonas membranes. Incubation of these membranes with activated cholera or pertussis toxin and [adenylate 32P]NAD+ led to incorporation of radioactivity into bands of about 40–44 kDa. Crude membranes were electrophoresed on SDS‐polyacrylamide gels and analyzed, by Western blotting, with the 9188 anti‐αs antibody and the AS/7 antibody (anti‐α1, anti‐αi1, anti‐αi2). These procedures resulted in the identification of polypeptides of approximately 40–44 kDa. Phytomonas adenylyl cyclase could be activated by treatment of membrane preparations with cholera toxin, in the presence of NAD+, while similar treatment with pertussis toxin did not affect this enzyme activity. These studies indicate that in Phytomonas, adenylyl cyclase activity is coupled to an unknown receptor entity through Gαs, proteins. Copyright © 1995, Wiley Blackwell. All rights reserved Fil:Farber, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paveto, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Flawia, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1995 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10665234_v42_n3_p257_Farber http://hdl.handle.net/20.500.12110/paper_10665234_v42_n3_p257_Farber |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
ADP‐ribosylation cholera toxin pertussis toxin plant trypanosomatids adenosine diphosphate adenylate cyclase adenylate cyclase toxin bacterial toxin Bordetella virulence factor cholera toxin divalent cation guanosine triphosphate heterotrimeric guanine nucleotide binding protein magnesium manganese nicotinamide adenine dinucleotide pertussis toxin animal article cell membrane cytology drug effect enzyme activation enzymology flagellate metabolism molecular weight protein binding Adenosine Diphosphate Adenylate Cyclase Adenylate Cyclase Toxin Animals Bacterial Toxins Cations, Divalent Cell Membrane Cholera Toxin Enzyme Activation Guanosine Triphosphate Heterotrimeric GTP-Binding Proteins Magnesium Manganese Molecular Weight NAD Pertussis Toxin Protein Binding Trypanosomatina Virulence Factors, Bordetella |
| spellingShingle |
ADP‐ribosylation cholera toxin pertussis toxin plant trypanosomatids adenosine diphosphate adenylate cyclase adenylate cyclase toxin bacterial toxin Bordetella virulence factor cholera toxin divalent cation guanosine triphosphate heterotrimeric guanine nucleotide binding protein magnesium manganese nicotinamide adenine dinucleotide pertussis toxin animal article cell membrane cytology drug effect enzyme activation enzymology flagellate metabolism molecular weight protein binding Adenosine Diphosphate Adenylate Cyclase Adenylate Cyclase Toxin Animals Bacterial Toxins Cations, Divalent Cell Membrane Cholera Toxin Enzyme Activation Guanosine Triphosphate Heterotrimeric GTP-Binding Proteins Magnesium Manganese Molecular Weight NAD Pertussis Toxin Protein Binding Trypanosomatina Virulence Factors, Bordetella Farber, Marisa Diana Paveto, María Cristina Torres, Héctor Norberto Flawiá, Mirtha María Adenyiyl Cyclase and G‐Proteins in Phytomonas |
| topic_facet |
ADP‐ribosylation cholera toxin pertussis toxin plant trypanosomatids adenosine diphosphate adenylate cyclase adenylate cyclase toxin bacterial toxin Bordetella virulence factor cholera toxin divalent cation guanosine triphosphate heterotrimeric guanine nucleotide binding protein magnesium manganese nicotinamide adenine dinucleotide pertussis toxin animal article cell membrane cytology drug effect enzyme activation enzymology flagellate metabolism molecular weight protein binding Adenosine Diphosphate Adenylate Cyclase Adenylate Cyclase Toxin Animals Bacterial Toxins Cations, Divalent Cell Membrane Cholera Toxin Enzyme Activation Guanosine Triphosphate Heterotrimeric GTP-Binding Proteins Magnesium Manganese Molecular Weight NAD Pertussis Toxin Protein Binding Trypanosomatina Virulence Factors, Bordetella |
| description |
ABSTRACT. Phytomonas sp. membranes have an adenylyl cyclase activity which is greater in the presence of Mn2+ than with Mg2+. The Mg2+ and Mn2+ activity ratio varies from one membrane preparation to another, suggesting that the adenylyl cyclase has a variable activation state. A [35S]GTP‐γ‐S‐binding activity with a Kd of 171 nM was detected in Phytomonas membranes. Incubation of these membranes with activated cholera or pertussis toxin and [adenylate 32P]NAD+ led to incorporation of radioactivity into bands of about 40–44 kDa. Crude membranes were electrophoresed on SDS‐polyacrylamide gels and analyzed, by Western blotting, with the 9188 anti‐αs antibody and the AS/7 antibody (anti‐α1, anti‐αi1, anti‐αi2). These procedures resulted in the identification of polypeptides of approximately 40–44 kDa. Phytomonas adenylyl cyclase could be activated by treatment of membrane preparations with cholera toxin, in the presence of NAD+, while similar treatment with pertussis toxin did not affect this enzyme activity. These studies indicate that in Phytomonas, adenylyl cyclase activity is coupled to an unknown receptor entity through Gαs, proteins. Copyright © 1995, Wiley Blackwell. All rights reserved |
| author |
Farber, Marisa Diana Paveto, María Cristina Torres, Héctor Norberto Flawiá, Mirtha María |
| author_facet |
Farber, Marisa Diana Paveto, María Cristina Torres, Héctor Norberto Flawiá, Mirtha María |
| author_sort |
Farber, Marisa Diana |
| title |
Adenyiyl Cyclase and G‐Proteins in Phytomonas |
| title_short |
Adenyiyl Cyclase and G‐Proteins in Phytomonas |
| title_full |
Adenyiyl Cyclase and G‐Proteins in Phytomonas |
| title_fullStr |
Adenyiyl Cyclase and G‐Proteins in Phytomonas |
| title_full_unstemmed |
Adenyiyl Cyclase and G‐Proteins in Phytomonas |
| title_sort |
adenyiyl cyclase and g‐proteins in phytomonas |
| publishDate |
1995 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10665234_v42_n3_p257_Farber http://hdl.handle.net/20.500.12110/paper_10665234_v42_n3_p257_Farber |
| work_keys_str_mv |
AT farbermarisadiana adenyiylcyclaseandgproteinsinphytomonas AT pavetomariacristina adenyiylcyclaseandgproteinsinphytomonas AT torreshectornorberto adenyiylcyclaseandgproteinsinphytomonas AT flawiamirthamaria adenyiylcyclaseandgproteinsinphytomonas |
| _version_ |
1768543287774281728 |