Novel DL-galactan hybrids from the red seaweed Gymnogongrus torulosus are potent inhibitors of herpes simplex virus and dengue virus

A novel series of DL-galactan hybrids extracted from the red seaweed Gymnogongrus torulosus, was evaluated for its in vitro antiviral properties against herpes simplex virus type 2 (HSV-2) and dengue virus 2 (DEN-2). These compounds were very active against both viruses with inhibitory concentration...

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Autores principales:	Pujol, Carlos Alberto, Estevez, Jose Manuel, Carlucci, María Josefina, Ciancia, Marina, Cerezo, Alberto Saúl, Damonte, Elsa Beatriz
Publicado:	2002
Materias:	Antiviral Dengue virus DL-glactans Herpes simplex virus Seaweed Sulfated polysaccharide antivirus agent carrageenan disaccharide galactan polysaccharide virus envelope protein virus glycoprotein animal cell anticoagulation antiviral activity article controlled study drug cytotoxicity drug isolation drug mechanism drug potency gymnogongrus torulosus Herpes simplex virus 2 in vitro study internalization nonhuman priority journal seaweed Vero cell virus adsorption virus inhibition virus plaque
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09563202_v13_n2_p83_Pujol http://hdl.handle.net/20.500.12110/paper_09563202_v13_n2_p83_Pujol
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_09563202_v13_n2_p83_Pujol
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spelling	paper:paper_09563202_v13_n2_p83_Pujol2023-06-08T15:55:59Z Novel DL-galactan hybrids from the red seaweed Gymnogongrus torulosus are potent inhibitors of herpes simplex virus and dengue virus Pujol, Carlos Alberto Estevez, Jose Manuel Carlucci, María Josefina Ciancia, Marina Cerezo, Alberto Saúl Damonte, Elsa Beatriz Antiviral Dengue virus DL-glactans Herpes simplex virus Seaweed Sulfated polysaccharide antivirus agent carrageenan disaccharide galactan polysaccharide virus envelope protein virus glycoprotein animal cell anticoagulation antiviral activity article controlled study Dengue virus drug cytotoxicity drug isolation drug mechanism drug potency gymnogongrus torulosus Herpes simplex virus 2 in vitro study internalization nonhuman priority journal seaweed Vero cell virus adsorption virus inhibition virus plaque A novel series of DL-galactan hybrids extracted from the red seaweed Gymnogongrus torulosus, was evaluated for its in vitro antiviral properties against herpes simplex virus type 2 (HSV-2) and dengue virus 2 (DEN-2). These compounds were very active against both viruses with inhibitory concentration 50% (IC50) values in the range 0.6-16 μg/ml for HSV-2 and 0.19-1.7 μg/ml for DEN-2, respectively, as determined in a virus plaque reduction assay in Vero cells. The DL-galactans lacked of cytotoxic effects, on stationary as well as on actively dividing cells, and anticoagulant properties. Some of the compounds showed a variable level of direct inactivating effect on both virions, with virucidal concentration 50% values exceeding the IC50S obtained by plaque reduction assay. Full inhibitory activity was achieved when the galactans were present during virus adsorption period, suggesting that the mode of action of these compounds is an interference in the binding of the surface envelope glycoprotein with the cell receptor. Fil:Pujol, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carlucci, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ciancia, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerezo, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09563202_v13_n2_p83_Pujol http://hdl.handle.net/20.500.12110/paper_09563202_v13_n2_p83_Pujol
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Antiviral Dengue virus DL-glactans Herpes simplex virus Seaweed Sulfated polysaccharide antivirus agent carrageenan disaccharide galactan polysaccharide virus envelope protein virus glycoprotein animal cell anticoagulation antiviral activity article controlled study Dengue virus drug cytotoxicity drug isolation drug mechanism drug potency gymnogongrus torulosus Herpes simplex virus 2 in vitro study internalization nonhuman priority journal seaweed Vero cell virus adsorption virus inhibition virus plaque
spellingShingle	Antiviral Dengue virus DL-glactans Herpes simplex virus Seaweed Sulfated polysaccharide antivirus agent carrageenan disaccharide galactan polysaccharide virus envelope protein virus glycoprotein animal cell anticoagulation antiviral activity article controlled study Dengue virus drug cytotoxicity drug isolation drug mechanism drug potency gymnogongrus torulosus Herpes simplex virus 2 in vitro study internalization nonhuman priority journal seaweed Vero cell virus adsorption virus inhibition virus plaque Pujol, Carlos Alberto Estevez, Jose Manuel Carlucci, María Josefina Ciancia, Marina Cerezo, Alberto Saúl Damonte, Elsa Beatriz Novel DL-galactan hybrids from the red seaweed Gymnogongrus torulosus are potent inhibitors of herpes simplex virus and dengue virus
topic_facet	Antiviral Dengue virus DL-glactans Herpes simplex virus Seaweed Sulfated polysaccharide antivirus agent carrageenan disaccharide galactan polysaccharide virus envelope protein virus glycoprotein animal cell anticoagulation antiviral activity article controlled study Dengue virus drug cytotoxicity drug isolation drug mechanism drug potency gymnogongrus torulosus Herpes simplex virus 2 in vitro study internalization nonhuman priority journal seaweed Vero cell virus adsorption virus inhibition virus plaque
description	A novel series of DL-galactan hybrids extracted from the red seaweed Gymnogongrus torulosus, was evaluated for its in vitro antiviral properties against herpes simplex virus type 2 (HSV-2) and dengue virus 2 (DEN-2). These compounds were very active against both viruses with inhibitory concentration 50% (IC50) values in the range 0.6-16 μg/ml for HSV-2 and 0.19-1.7 μg/ml for DEN-2, respectively, as determined in a virus plaque reduction assay in Vero cells. The DL-galactans lacked of cytotoxic effects, on stationary as well as on actively dividing cells, and anticoagulant properties. Some of the compounds showed a variable level of direct inactivating effect on both virions, with virucidal concentration 50% values exceeding the IC50S obtained by plaque reduction assay. Full inhibitory activity was achieved when the galactans were present during virus adsorption period, suggesting that the mode of action of these compounds is an interference in the binding of the surface envelope glycoprotein with the cell receptor.
author	Pujol, Carlos Alberto Estevez, Jose Manuel Carlucci, María Josefina Ciancia, Marina Cerezo, Alberto Saúl Damonte, Elsa Beatriz
author_facet	Pujol, Carlos Alberto Estevez, Jose Manuel Carlucci, María Josefina Ciancia, Marina Cerezo, Alberto Saúl Damonte, Elsa Beatriz
author_sort	Pujol, Carlos Alberto
title	Novel DL-galactan hybrids from the red seaweed Gymnogongrus torulosus are potent inhibitors of herpes simplex virus and dengue virus
title_short	Novel DL-galactan hybrids from the red seaweed Gymnogongrus torulosus are potent inhibitors of herpes simplex virus and dengue virus
title_full	Novel DL-galactan hybrids from the red seaweed Gymnogongrus torulosus are potent inhibitors of herpes simplex virus and dengue virus
title_fullStr	Novel DL-galactan hybrids from the red seaweed Gymnogongrus torulosus are potent inhibitors of herpes simplex virus and dengue virus
title_full_unstemmed	Novel DL-galactan hybrids from the red seaweed Gymnogongrus torulosus are potent inhibitors of herpes simplex virus and dengue virus
title_sort	novel dl-galactan hybrids from the red seaweed gymnogongrus torulosus are potent inhibitors of herpes simplex virus and dengue virus
publishDate	2002
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09563202_v13_n2_p83_Pujol http://hdl.handle.net/20.500.12110/paper_09563202_v13_n2_p83_Pujol
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Novel DL-galactan hybrids from the red seaweed Gymnogongrus torulosus are potent inhibitors of herpes simplex virus and dengue virus

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