Interleukin-6 is inhibited by glucocorticoids and stimulates ACTH secretion and POMC expression in human corticotroph pituitary adenomas

Interleukins and their receptors are expressed intrinsically in the anterior pituitary and regulate hormone production and cell proliferation. It has previously been shown that interleukin-6 (IL-6) regulates hormone secretion in normal pituitary cells and cell lines. Here we examined the effects of...

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Detalles Bibliográficos
Autores principales: Páez Pereda, Marcelo, Kovalovsky, Damián, Perez Castro, Carolina Inés
Publicado: 2000
Materias:
ACTH
Corticotroph adenoma
Interleukin-6
Proopiomelanocortin
corticotropin
dexamethasone
interleukin 6
proopiomelanocortin
adult
article
clinical article
controlled study
cytokine release
female
gene expression
hormone release
human
human cell
human tissue
hypophysis tumor
male
priority journal
Adenoma
Adrenocorticotropic Hormone
Adult
Cushing Syndrome
Female
Gene Expression Regulation, Neoplastic
Humans
Male
Middle Aged
Pituitary Neoplasms
Pro-Opiomelanocortin
Recombinant Proteins
RNA, Messenger
Transcription, Genetic
Tumor Cells, Cultured
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09477349_v108_n3_p202_PaezPereda
http://hdl.handle.net/20.500.12110/paper_09477349_v108_n3_p202_PaezPereda
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Interleukins and their receptors are expressed intrinsically in the anterior pituitary and regulate hormone production and cell proliferation. It has previously been shown that interleukin-6 (IL-6) regulates hormone secretion in normal pituitary cells and cell lines. Here we examined the effects of IL-6 on propiomelanocortin (POMC) expression and ACTH production in corticotroph adenoma cells in vitro. We found that IL-6 stimulates both ACTH secretion and POMC gene expression in corticotroph adenoma cell cultures. This first demonstration of the stimulatory action of IL-6 on human corticotroph adenoma cell function provides further evidence for a direct action of IL-6 on corticotroph pituitary cells. We have confirmed previous reports of IL-6 production by corticotroph adenoma cells and in addition, demonstrated for the first time that the synthetic glucocorticoid dexamethasone is a potent suppressor of intratumoral IL-6 production. This intratumoral produced IL-6 may be in part responsible, in an autocrine manner, for the stimulation of ACTH synthesis and secretion. Our results suggest that IL-6 might play a role in the pathogenesis of Cushing's disease. However, elevated glucocorticoid levels in patients with Cushing's disease may prevent excessive action of IL-6 on ACTH production and tumor progression of corticotroph adenomas in vivo.

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