Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities
The development of bacterial communities in replicate lab-scale-activated sludge reactors degrading a non-ionic surfactant was evaluated by statistical analysis of denaturing gradient gel electrophoresis (DGGE) fingerprints. Four sequential batch reactors were fed with synthetic sewage, two of which...
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09232508_v158_n8-9_p712_Lozada http://hdl.handle.net/20.500.12110/paper_09232508_v158_n8-9_p712_Lozada |
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paper:paper_09232508_v158_n8-9_p712_Lozada2023-06-08T15:50:58Z Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities Basile, Laura Ana Erijman, Leonardo Community structure Denaturing gradient gel electrophoresis Fingerprinting Microbial ecology Nonylphenol ethoxylates nonionic surfactant phenol derivative activated sludge article bacterial flora community structure denaturing gradient gel electrophoresis fed batch reactor nonhuman priority journal sequencing batch reactor statistical analysis Bacteria Culture Media, Conditioned Detergents Electrophoresis Ethylene Glycols Sewage Bacteria (microorganisms) The development of bacterial communities in replicate lab-scale-activated sludge reactors degrading a non-ionic surfactant was evaluated by statistical analysis of denaturing gradient gel electrophoresis (DGGE) fingerprints. Four sequential batch reactors were fed with synthetic sewage, two of which received, in addition, 0.01% of nonylphenol ethoxylates (NPE). The dynamic character of bacterial community structure was confirmed by the differences in species composition among replicate reactors. Measurement of similarities between reactors was obtained by pairwise similarity analysis using the Bray Curtis coefficient. The group of NPE-amended reactors exhibited the highest similarity values (Sjk = 0.53 ± 0.03), indicating that the bacterial community structure of NPE-amended reactors was better replicated than control reactors (Sjk = 0.36 ± 0.04). Replicate NPE-amended reactors taken at different times of operation clustered together, whereas analogous relations within the control reactor cluster were not observed. The DGGE pattern of isolates grown in conditioned media prepared with media taken at the end of the aeration cycle grouped separately from other conditioned and synthetic media regardless of the carbon source amendment, suggesting that NPE degradation residuals could have a role in the shaping of the community structure. © 2007 Elsevier Masson SAS. All rights reserved. Fil:Basile, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Erijman, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09232508_v158_n8-9_p712_Lozada http://hdl.handle.net/20.500.12110/paper_09232508_v158_n8-9_p712_Lozada |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Community structure Denaturing gradient gel electrophoresis Fingerprinting Microbial ecology Nonylphenol ethoxylates nonionic surfactant phenol derivative activated sludge article bacterial flora community structure denaturing gradient gel electrophoresis fed batch reactor nonhuman priority journal sequencing batch reactor statistical analysis Bacteria Culture Media, Conditioned Detergents Electrophoresis Ethylene Glycols Sewage Bacteria (microorganisms) |
spellingShingle |
Community structure Denaturing gradient gel electrophoresis Fingerprinting Microbial ecology Nonylphenol ethoxylates nonionic surfactant phenol derivative activated sludge article bacterial flora community structure denaturing gradient gel electrophoresis fed batch reactor nonhuman priority journal sequencing batch reactor statistical analysis Bacteria Culture Media, Conditioned Detergents Electrophoresis Ethylene Glycols Sewage Bacteria (microorganisms) Basile, Laura Ana Erijman, Leonardo Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities |
topic_facet |
Community structure Denaturing gradient gel electrophoresis Fingerprinting Microbial ecology Nonylphenol ethoxylates nonionic surfactant phenol derivative activated sludge article bacterial flora community structure denaturing gradient gel electrophoresis fed batch reactor nonhuman priority journal sequencing batch reactor statistical analysis Bacteria Culture Media, Conditioned Detergents Electrophoresis Ethylene Glycols Sewage Bacteria (microorganisms) |
description |
The development of bacterial communities in replicate lab-scale-activated sludge reactors degrading a non-ionic surfactant was evaluated by statistical analysis of denaturing gradient gel electrophoresis (DGGE) fingerprints. Four sequential batch reactors were fed with synthetic sewage, two of which received, in addition, 0.01% of nonylphenol ethoxylates (NPE). The dynamic character of bacterial community structure was confirmed by the differences in species composition among replicate reactors. Measurement of similarities between reactors was obtained by pairwise similarity analysis using the Bray Curtis coefficient. The group of NPE-amended reactors exhibited the highest similarity values (Sjk = 0.53 ± 0.03), indicating that the bacterial community structure of NPE-amended reactors was better replicated than control reactors (Sjk = 0.36 ± 0.04). Replicate NPE-amended reactors taken at different times of operation clustered together, whereas analogous relations within the control reactor cluster were not observed. The DGGE pattern of isolates grown in conditioned media prepared with media taken at the end of the aeration cycle grouped separately from other conditioned and synthetic media regardless of the carbon source amendment, suggesting that NPE degradation residuals could have a role in the shaping of the community structure. © 2007 Elsevier Masson SAS. All rights reserved. |
author |
Basile, Laura Ana Erijman, Leonardo |
author_facet |
Basile, Laura Ana Erijman, Leonardo |
author_sort |
Basile, Laura Ana |
title |
Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities |
title_short |
Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities |
title_full |
Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities |
title_fullStr |
Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities |
title_full_unstemmed |
Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities |
title_sort |
impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities |
publishDate |
2007 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09232508_v158_n8-9_p712_Lozada http://hdl.handle.net/20.500.12110/paper_09232508_v158_n8-9_p712_Lozada |
work_keys_str_mv |
AT basilelauraana impactofnonionicsurfactantonthelongtermdevelopmentoflabscaleactivatedsludgebacterialcommunities AT erijmanleonardo impactofnonionicsurfactantonthelongtermdevelopmentoflabscaleactivatedsludgebacterialcommunities |
_version_ |
1768543429121277952 |