Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities

The development of bacterial communities in replicate lab-scale-activated sludge reactors degrading a non-ionic surfactant was evaluated by statistical analysis of denaturing gradient gel electrophoresis (DGGE) fingerprints. Four sequential batch reactors were fed with synthetic sewage, two of which...

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Autores principales: Basile, Laura Ana, Erijman, Leonardo
Publicado: 2007
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09232508_v158_n8-9_p712_Lozada
http://hdl.handle.net/20.500.12110/paper_09232508_v158_n8-9_p712_Lozada
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spelling paper:paper_09232508_v158_n8-9_p712_Lozada2023-06-08T15:50:58Z Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities Basile, Laura Ana Erijman, Leonardo Community structure Denaturing gradient gel electrophoresis Fingerprinting Microbial ecology Nonylphenol ethoxylates nonionic surfactant phenol derivative activated sludge article bacterial flora community structure denaturing gradient gel electrophoresis fed batch reactor nonhuman priority journal sequencing batch reactor statistical analysis Bacteria Culture Media, Conditioned Detergents Electrophoresis Ethylene Glycols Sewage Bacteria (microorganisms) The development of bacterial communities in replicate lab-scale-activated sludge reactors degrading a non-ionic surfactant was evaluated by statistical analysis of denaturing gradient gel electrophoresis (DGGE) fingerprints. Four sequential batch reactors were fed with synthetic sewage, two of which received, in addition, 0.01% of nonylphenol ethoxylates (NPE). The dynamic character of bacterial community structure was confirmed by the differences in species composition among replicate reactors. Measurement of similarities between reactors was obtained by pairwise similarity analysis using the Bray Curtis coefficient. The group of NPE-amended reactors exhibited the highest similarity values (Sjk = 0.53 ± 0.03), indicating that the bacterial community structure of NPE-amended reactors was better replicated than control reactors (Sjk = 0.36 ± 0.04). Replicate NPE-amended reactors taken at different times of operation clustered together, whereas analogous relations within the control reactor cluster were not observed. The DGGE pattern of isolates grown in conditioned media prepared with media taken at the end of the aeration cycle grouped separately from other conditioned and synthetic media regardless of the carbon source amendment, suggesting that NPE degradation residuals could have a role in the shaping of the community structure. © 2007 Elsevier Masson SAS. All rights reserved. Fil:Basile, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Erijman, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09232508_v158_n8-9_p712_Lozada http://hdl.handle.net/20.500.12110/paper_09232508_v158_n8-9_p712_Lozada
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Community structure
Denaturing gradient gel electrophoresis
Fingerprinting
Microbial ecology
Nonylphenol ethoxylates
nonionic surfactant
phenol derivative
activated sludge
article
bacterial flora
community structure
denaturing gradient gel electrophoresis
fed batch reactor
nonhuman
priority journal
sequencing batch reactor
statistical analysis
Bacteria
Culture Media, Conditioned
Detergents
Electrophoresis
Ethylene Glycols
Sewage
Bacteria (microorganisms)
spellingShingle Community structure
Denaturing gradient gel electrophoresis
Fingerprinting
Microbial ecology
Nonylphenol ethoxylates
nonionic surfactant
phenol derivative
activated sludge
article
bacterial flora
community structure
denaturing gradient gel electrophoresis
fed batch reactor
nonhuman
priority journal
sequencing batch reactor
statistical analysis
Bacteria
Culture Media, Conditioned
Detergents
Electrophoresis
Ethylene Glycols
Sewage
Bacteria (microorganisms)
Basile, Laura Ana
Erijman, Leonardo
Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities
topic_facet Community structure
Denaturing gradient gel electrophoresis
Fingerprinting
Microbial ecology
Nonylphenol ethoxylates
nonionic surfactant
phenol derivative
activated sludge
article
bacterial flora
community structure
denaturing gradient gel electrophoresis
fed batch reactor
nonhuman
priority journal
sequencing batch reactor
statistical analysis
Bacteria
Culture Media, Conditioned
Detergents
Electrophoresis
Ethylene Glycols
Sewage
Bacteria (microorganisms)
description The development of bacterial communities in replicate lab-scale-activated sludge reactors degrading a non-ionic surfactant was evaluated by statistical analysis of denaturing gradient gel electrophoresis (DGGE) fingerprints. Four sequential batch reactors were fed with synthetic sewage, two of which received, in addition, 0.01% of nonylphenol ethoxylates (NPE). The dynamic character of bacterial community structure was confirmed by the differences in species composition among replicate reactors. Measurement of similarities between reactors was obtained by pairwise similarity analysis using the Bray Curtis coefficient. The group of NPE-amended reactors exhibited the highest similarity values (Sjk = 0.53 ± 0.03), indicating that the bacterial community structure of NPE-amended reactors was better replicated than control reactors (Sjk = 0.36 ± 0.04). Replicate NPE-amended reactors taken at different times of operation clustered together, whereas analogous relations within the control reactor cluster were not observed. The DGGE pattern of isolates grown in conditioned media prepared with media taken at the end of the aeration cycle grouped separately from other conditioned and synthetic media regardless of the carbon source amendment, suggesting that NPE degradation residuals could have a role in the shaping of the community structure. © 2007 Elsevier Masson SAS. All rights reserved.
author Basile, Laura Ana
Erijman, Leonardo
author_facet Basile, Laura Ana
Erijman, Leonardo
author_sort Basile, Laura Ana
title Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities
title_short Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities
title_full Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities
title_fullStr Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities
title_full_unstemmed Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities
title_sort impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities
publishDate 2007
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09232508_v158_n8-9_p712_Lozada
http://hdl.handle.net/20.500.12110/paper_09232508_v158_n8-9_p712_Lozada
work_keys_str_mv AT basilelauraana impactofnonionicsurfactantonthelongtermdevelopmentoflabscaleactivatedsludgebacterialcommunities
AT erijmanleonardo impactofnonionicsurfactantonthelongtermdevelopmentoflabscaleactivatedsludgebacterialcommunities
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