Influence of the spray adjuvant on the toxicity effects of a glyphosate formulation

In the present study, the influence of the spray adjuvant on the toxicity effects of a glyphosate formulation was examined in HEp-2 cell line. We determined the median lethal concentration (LC50) of Atanor® (glyphosate formulation), Impacto® (spray adjuvant) and the mixture of both agrochemicals. We...

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Autores principales:	Coalova, Isis, Ríos de Molina, María del Carmen, Chaufan, Rosa Gabriela
Publicado:	2014
Materias:	In vitro Mixture toxicity Oxidative stress Spray adjuvant adjuvant caspase 3 caspase 7 catalase glutathione glutathione transferase glyphosate reactive oxygen metabolite superoxide dismutase glycine herbicide aerosol antioxidant activity apoptosis article cell culture controlled study cytotoxicity dose response enzyme activity HEp 2 cell line human human cell LC 50 oxidative stress protein synthesis signal transduction timed drug release analogs and derivatives cell survival chemistry drug effects medicinal chemistry metabolism tumor cell line Caspase 3 Caspase 7 Catalase Cell Line, Tumor Cell Survival Chemistry, Pharmaceutical Glutathione Transferase Glycine Herbicides Humans Reactive Oxygen Species Superoxide Dismutase
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08872333_v28_n7_p1306_Coalova http://hdl.handle.net/20.500.12110/paper_08872333_v28_n7_p1306_Coalova
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_08872333_v28_n7_p1306_Coalova
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spelling	paper:paper_08872333_v28_n7_p1306_Coalova2023-06-08T15:46:45Z Influence of the spray adjuvant on the toxicity effects of a glyphosate formulation Coalova, Isis Ríos de Molina, María del Carmen Chaufan, Rosa Gabriela In vitro Mixture toxicity Oxidative stress Spray adjuvant adjuvant caspase 3 caspase 7 catalase glutathione glutathione transferase glyphosate reactive oxygen metabolite superoxide dismutase caspase 3 caspase 7 catalase glutathione transferase glycine glyphosate herbicide reactive oxygen metabolite superoxide dismutase aerosol antioxidant activity apoptosis article cell culture controlled study cytotoxicity dose response enzyme activity HEp 2 cell line human human cell LC 50 oxidative stress protein synthesis signal transduction timed drug release analogs and derivatives cell survival chemistry drug effects medicinal chemistry metabolism tumor cell line Caspase 3 Caspase 7 Catalase Cell Line, Tumor Cell Survival Chemistry, Pharmaceutical Glutathione Transferase Glycine Herbicides Humans Reactive Oxygen Species Superoxide Dismutase In the present study, the influence of the spray adjuvant on the toxicity effects of a glyphosate formulation was examined in HEp-2 cell line. We determined the median lethal concentration (LC50) of Atanor® (glyphosate formulation), Impacto® (spray adjuvant) and the mixture of both agrochemicals. We also compared the toxicities of the pesticides individually and in mixture and we analyzed the effects on oxidative balance from each treatment.Our results showed that all the agrochemicals assayed induce dose and time-dependent cytotoxicity and that the toxicity of Impacto® with Atanor® (mixture) was additive on HEp-2 cell line.All the agrochemicals assayed produced an increase in catalase activity and glutathione levels, while no effects were observed for superoxide dismutase and glutathione-S-transferase activities. We found an important increase in ROS production in cells treated with Atanor® and mixture. Besides, all the agrochemicals used triggered caspase 3/7 activation and hence induced apoptosis pathway in this cell line. In conclusion, our results demonstrated that the addition of adjuvant to glyphosate formulation increase the toxicity of the mixture in cell culture. Furthermore, cell culture exposed to agrochemical mixture showed an increased ROS production and antioxidant defenses. © 2014 Elsevier Ltd. Fil:Coalova, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ríos de Molina, M.D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Chaufan, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08872333_v28_n7_p1306_Coalova http://hdl.handle.net/20.500.12110/paper_08872333_v28_n7_p1306_Coalova
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	In vitro Mixture toxicity Oxidative stress Spray adjuvant adjuvant caspase 3 caspase 7 catalase glutathione glutathione transferase glyphosate reactive oxygen metabolite superoxide dismutase caspase 3 caspase 7 catalase glutathione transferase glycine glyphosate herbicide reactive oxygen metabolite superoxide dismutase aerosol antioxidant activity apoptosis article cell culture controlled study cytotoxicity dose response enzyme activity HEp 2 cell line human human cell LC 50 oxidative stress protein synthesis signal transduction timed drug release analogs and derivatives cell survival chemistry drug effects medicinal chemistry metabolism tumor cell line Caspase 3 Caspase 7 Catalase Cell Line, Tumor Cell Survival Chemistry, Pharmaceutical Glutathione Transferase Glycine Herbicides Humans Reactive Oxygen Species Superoxide Dismutase
spellingShingle	In vitro Mixture toxicity Oxidative stress Spray adjuvant adjuvant caspase 3 caspase 7 catalase glutathione glutathione transferase glyphosate reactive oxygen metabolite superoxide dismutase caspase 3 caspase 7 catalase glutathione transferase glycine glyphosate herbicide reactive oxygen metabolite superoxide dismutase aerosol antioxidant activity apoptosis article cell culture controlled study cytotoxicity dose response enzyme activity HEp 2 cell line human human cell LC 50 oxidative stress protein synthesis signal transduction timed drug release analogs and derivatives cell survival chemistry drug effects medicinal chemistry metabolism tumor cell line Caspase 3 Caspase 7 Catalase Cell Line, Tumor Cell Survival Chemistry, Pharmaceutical Glutathione Transferase Glycine Herbicides Humans Reactive Oxygen Species Superoxide Dismutase Coalova, Isis Ríos de Molina, María del Carmen Chaufan, Rosa Gabriela Influence of the spray adjuvant on the toxicity effects of a glyphosate formulation
topic_facet	In vitro Mixture toxicity Oxidative stress Spray adjuvant adjuvant caspase 3 caspase 7 catalase glutathione glutathione transferase glyphosate reactive oxygen metabolite superoxide dismutase caspase 3 caspase 7 catalase glutathione transferase glycine glyphosate herbicide reactive oxygen metabolite superoxide dismutase aerosol antioxidant activity apoptosis article cell culture controlled study cytotoxicity dose response enzyme activity HEp 2 cell line human human cell LC 50 oxidative stress protein synthesis signal transduction timed drug release analogs and derivatives cell survival chemistry drug effects medicinal chemistry metabolism tumor cell line Caspase 3 Caspase 7 Catalase Cell Line, Tumor Cell Survival Chemistry, Pharmaceutical Glutathione Transferase Glycine Herbicides Humans Reactive Oxygen Species Superoxide Dismutase
description	In the present study, the influence of the spray adjuvant on the toxicity effects of a glyphosate formulation was examined in HEp-2 cell line. We determined the median lethal concentration (LC50) of Atanor® (glyphosate formulation), Impacto® (spray adjuvant) and the mixture of both agrochemicals. We also compared the toxicities of the pesticides individually and in mixture and we analyzed the effects on oxidative balance from each treatment.Our results showed that all the agrochemicals assayed induce dose and time-dependent cytotoxicity and that the toxicity of Impacto® with Atanor® (mixture) was additive on HEp-2 cell line.All the agrochemicals assayed produced an increase in catalase activity and glutathione levels, while no effects were observed for superoxide dismutase and glutathione-S-transferase activities. We found an important increase in ROS production in cells treated with Atanor® and mixture. Besides, all the agrochemicals used triggered caspase 3/7 activation and hence induced apoptosis pathway in this cell line. In conclusion, our results demonstrated that the addition of adjuvant to glyphosate formulation increase the toxicity of the mixture in cell culture. Furthermore, cell culture exposed to agrochemical mixture showed an increased ROS production and antioxidant defenses. © 2014 Elsevier Ltd.
author	Coalova, Isis Ríos de Molina, María del Carmen Chaufan, Rosa Gabriela
author_facet	Coalova, Isis Ríos de Molina, María del Carmen Chaufan, Rosa Gabriela
author_sort	Coalova, Isis
title	Influence of the spray adjuvant on the toxicity effects of a glyphosate formulation
title_short	Influence of the spray adjuvant on the toxicity effects of a glyphosate formulation
title_full	Influence of the spray adjuvant on the toxicity effects of a glyphosate formulation
title_fullStr	Influence of the spray adjuvant on the toxicity effects of a glyphosate formulation
title_full_unstemmed	Influence of the spray adjuvant on the toxicity effects of a glyphosate formulation
title_sort	influence of the spray adjuvant on the toxicity effects of a glyphosate formulation
publishDate	2014
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08872333_v28_n7_p1306_Coalova http://hdl.handle.net/20.500.12110/paper_08872333_v28_n7_p1306_Coalova
work_keys_str_mv	AT coalovaisis influenceofthesprayadjuvantonthetoxicityeffectsofaglyphosateformulation AT riosdemolinamariadelcarmen influenceofthesprayadjuvantonthetoxicityeffectsofaglyphosateformulation AT chaufanrosagabriela influenceofthesprayadjuvantonthetoxicityeffectsofaglyphosateformulation
_version_	1768544476729442304

Influence of the spray adjuvant on the toxicity effects of a glyphosate formulation

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