Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH-GISH)

The karyotype of Elymus scabrifolius (Döll) J.H. Hunz. (2n = 4x = 28) was investigated by DAPI staining and in situ hybridization. All the accessions studied presented a symmetric and uniform karyotype constituted by 9m+2m-sm+3sm. DAPI stain showed 1-7 conspicuous bands in all the chromosomes and po...

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Autores principales:	Tomas, Pablo Andrés, González, Graciela Esther, Poggio, Lidia
Publicado:	2012
Materias:	Elymus scabrifolius FISH GISH intergenomic rearrangement karyotype ribosome DNA Argentina article chemistry chromosome analysis fluorescence in situ hybridization genetics karyotyping nucleic acid hybridization plant genome Poaceae Cytogenetic Analysis DNA, Ribosomal Genome, Plant In Situ Hybridization, Fluorescence Karyotyping Nucleic Acid Hybridization
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v55_n8_p591_Tomas http://hdl.handle.net/20.500.12110/paper_08312796_v55_n8_p591_Tomas
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_08312796_v55_n8_p591_Tomas
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spelling	paper:paper_08312796_v55_n8_p591_Tomas2023-06-08T15:46:18Z Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH-GISH) Tomas, Pablo Andrés González, Graciela Esther Poggio, Lidia Elymus scabrifolius FISH GISH intergenomic rearrangement karyotype ribosome DNA Argentina article chemistry chromosome analysis fluorescence in situ hybridization genetics karyotyping nucleic acid hybridization plant genome Poaceae Argentina Cytogenetic Analysis DNA, Ribosomal Genome, Plant In Situ Hybridization, Fluorescence Karyotyping Nucleic Acid Hybridization Poaceae The karyotype of Elymus scabrifolius (Döll) J.H. Hunz. (2n = 4x = 28) was investigated by DAPI staining and in situ hybridization. All the accessions studied presented a symmetric and uniform karyotype constituted by 9m+2m-sm+3sm. DAPI stain showed 1-7 conspicuous bands in all the chromosomes and polymorphisms between accessions. FISH experiments carried out with 45S rDNA as probe (pTa71) showed strong hybridization signals on the metacentric SAT-chromosome pair 8; the submetacentric SAT-chromosome pair 13 presented weaker hybridization. FISH using pSc119.2 clone as probe identified five chromosome pairs. Then, the combination of chromosome morphology, DAPI-staining, and FISH enabled the accurate identification of each chromosome pair in E. scabrifolius. Genomic in situ hybridization (GISH) experiments using Hordeum DNA as probe on mitotic metaphases confirmed unequivocally the presence of the H genome in E. scabrifolius, allowing us to observe six uniformly labeled chromosome pairs and two chromosome pairs with only one arm labeled. The remaining six chromosome pairs were weakly labeled. The rehybridization of FISH slides with Hordeum DNA as probe allow us to assign the genomic provenance of most of the chromosomes in the studied accessions. Moreover, intergenomic rearrangement was detected between genome H and the still unknown progenitor genome. © 2012 Published by NRC Research Press. Fil:Tomas, P.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:González, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Poggio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v55_n8_p591_Tomas http://hdl.handle.net/20.500.12110/paper_08312796_v55_n8_p591_Tomas
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Elymus scabrifolius FISH GISH intergenomic rearrangement karyotype ribosome DNA Argentina article chemistry chromosome analysis fluorescence in situ hybridization genetics karyotyping nucleic acid hybridization plant genome Poaceae Argentina Cytogenetic Analysis DNA, Ribosomal Genome, Plant In Situ Hybridization, Fluorescence Karyotyping Nucleic Acid Hybridization Poaceae
spellingShingle	Elymus scabrifolius FISH GISH intergenomic rearrangement karyotype ribosome DNA Argentina article chemistry chromosome analysis fluorescence in situ hybridization genetics karyotyping nucleic acid hybridization plant genome Poaceae Argentina Cytogenetic Analysis DNA, Ribosomal Genome, Plant In Situ Hybridization, Fluorescence Karyotyping Nucleic Acid Hybridization Poaceae Tomas, Pablo Andrés González, Graciela Esther Poggio, Lidia Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH-GISH)
topic_facet	Elymus scabrifolius FISH GISH intergenomic rearrangement karyotype ribosome DNA Argentina article chemistry chromosome analysis fluorescence in situ hybridization genetics karyotyping nucleic acid hybridization plant genome Poaceae Argentina Cytogenetic Analysis DNA, Ribosomal Genome, Plant In Situ Hybridization, Fluorescence Karyotyping Nucleic Acid Hybridization Poaceae
description	The karyotype of Elymus scabrifolius (Döll) J.H. Hunz. (2n = 4x = 28) was investigated by DAPI staining and in situ hybridization. All the accessions studied presented a symmetric and uniform karyotype constituted by 9m+2m-sm+3sm. DAPI stain showed 1-7 conspicuous bands in all the chromosomes and polymorphisms between accessions. FISH experiments carried out with 45S rDNA as probe (pTa71) showed strong hybridization signals on the metacentric SAT-chromosome pair 8; the submetacentric SAT-chromosome pair 13 presented weaker hybridization. FISH using pSc119.2 clone as probe identified five chromosome pairs. Then, the combination of chromosome morphology, DAPI-staining, and FISH enabled the accurate identification of each chromosome pair in E. scabrifolius. Genomic in situ hybridization (GISH) experiments using Hordeum DNA as probe on mitotic metaphases confirmed unequivocally the presence of the H genome in E. scabrifolius, allowing us to observe six uniformly labeled chromosome pairs and two chromosome pairs with only one arm labeled. The remaining six chromosome pairs were weakly labeled. The rehybridization of FISH slides with Hordeum DNA as probe allow us to assign the genomic provenance of most of the chromosomes in the studied accessions. Moreover, intergenomic rearrangement was detected between genome H and the still unknown progenitor genome. © 2012 Published by NRC Research Press.
author	Tomas, Pablo Andrés González, Graciela Esther Poggio, Lidia
author_facet	Tomas, Pablo Andrés González, Graciela Esther Poggio, Lidia
author_sort	Tomas, Pablo Andrés
title	Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH-GISH)
title_short	Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH-GISH)
title_full	Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH-GISH)
title_fullStr	Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH-GISH)
title_full_unstemmed	Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH-GISH)
title_sort	chromosomal characterization in native populations of elymus scabrifolius from argentina through classical and molecular cytogenetics (fish-gish)
publishDate	2012
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v55_n8_p591_Tomas http://hdl.handle.net/20.500.12110/paper_08312796_v55_n8_p591_Tomas
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Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH-GISH)

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