Genomic affinities of Zea luxurians, Z. diploperennis, And Z. perennis: Meiotic behavior of their F1 hybrids and genomic in situ hybridization (GISH)
Since 1987 cytological evidence has arisen in our laboratory, pointing to x = 5 as the original basic chromosome number of maize and its related wild species. This paper deals with the analysis of the meiotic behavior of F1 hybrids Zea luxurians x Z. diploperennis (2n = 20) and Z. luxurians x Z. per...
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1999
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v42_n5_p993_Poggio http://hdl.handle.net/20.500.12110/paper_08312796_v42_n5_p993_Poggio |
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paper:paper_08312796_v42_n5_p993_Poggio2023-06-08T15:46:15Z Genomic affinities of Zea luxurians, Z. diploperennis, And Z. perennis: Meiotic behavior of their F1 hybrids and genomic in situ hybridization (GISH) Poggio, Lidia Confalonieri, Viviana Andrea Comas, Cecilia Isabel González, Graciela Esther Naranjo, Carlos Alberto Cytogenetics Evolution Heterochromatic knobs Repetitive sequences Zea hybrids 4',6 diamidino 2 phenylindole chromosome analysis chromosome banding chromosome number fluorescence gene probe genetic marker genome hybrid in situ hybridization meiosis metaphase mitosis plant genome plant taxonomy repetitive DNA telomere wild relative Zea diploperennis Zea luxurians Zea perennis Since 1987 cytological evidence has arisen in our laboratory, pointing to x = 5 as the original basic chromosome number of maize and its related wild species. This paper deals with the analysis of the meiotic behavior of F1 hybrids Zea luxurians x Z. diploperennis (2n = 20) and Z. luxurians x Z. perennis (2n = 30). In the first hybrid the most frequent configuration was 811 + 41 and in the latter was 5111 + 511 + 51. Applying GISH (genomic in situ hybridization) to mitotic chromosomes of Z. luxurians we found that DAPI (4',6-diamidino-2-phenylindole) positive bands located in all telomeric regions of this species did not hybridize with either Z. perennis or Z diploperennis genomic probe. Therefore, Z. luxurians has a repetitive sequence that can be used in fluorescent staining to identify its chromosomes. When GISH was employed on metaphase I of the 2n = 30 hybrid, all the univalents showed distinctive telomeres of Z. luxurians, while the bivalents did not present any signal. These findings show that the formation of bivalent-univalent configurations is not a random event. The bivalents tend to be spatially separated and are very often observed forming an independent group of 5II. Finally, trivalents were composed by one chromosome labeled in its telomeric regions, and two smaller and unlabeled ones. The use of chromosome markers of Z. luxurians demonstrated to be a good step forward in interpreting the nature of meiotic configurations in 2n = 30 Zea spp. hybrids. They can help to clarify the relationship between genomes and provide a useful addition to the taxonomic classification in the genus Zea. Fil:Poggio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Confalonieri, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Comas, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gonzalez, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Naranjo, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1999 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v42_n5_p993_Poggio http://hdl.handle.net/20.500.12110/paper_08312796_v42_n5_p993_Poggio |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cytogenetics Evolution Heterochromatic knobs Repetitive sequences Zea hybrids 4',6 diamidino 2 phenylindole chromosome analysis chromosome banding chromosome number fluorescence gene probe genetic marker genome hybrid in situ hybridization meiosis metaphase mitosis plant genome plant taxonomy repetitive DNA telomere wild relative Zea diploperennis Zea luxurians Zea perennis |
| spellingShingle |
Cytogenetics Evolution Heterochromatic knobs Repetitive sequences Zea hybrids 4',6 diamidino 2 phenylindole chromosome analysis chromosome banding chromosome number fluorescence gene probe genetic marker genome hybrid in situ hybridization meiosis metaphase mitosis plant genome plant taxonomy repetitive DNA telomere wild relative Zea diploperennis Zea luxurians Zea perennis Poggio, Lidia Confalonieri, Viviana Andrea Comas, Cecilia Isabel González, Graciela Esther Naranjo, Carlos Alberto Genomic affinities of Zea luxurians, Z. diploperennis, And Z. perennis: Meiotic behavior of their F1 hybrids and genomic in situ hybridization (GISH) |
| topic_facet |
Cytogenetics Evolution Heterochromatic knobs Repetitive sequences Zea hybrids 4',6 diamidino 2 phenylindole chromosome analysis chromosome banding chromosome number fluorescence gene probe genetic marker genome hybrid in situ hybridization meiosis metaphase mitosis plant genome plant taxonomy repetitive DNA telomere wild relative Zea diploperennis Zea luxurians Zea perennis |
| description |
Since 1987 cytological evidence has arisen in our laboratory, pointing to x = 5 as the original basic chromosome number of maize and its related wild species. This paper deals with the analysis of the meiotic behavior of F1 hybrids Zea luxurians x Z. diploperennis (2n = 20) and Z. luxurians x Z. perennis (2n = 30). In the first hybrid the most frequent configuration was 811 + 41 and in the latter was 5111 + 511 + 51. Applying GISH (genomic in situ hybridization) to mitotic chromosomes of Z. luxurians we found that DAPI (4',6-diamidino-2-phenylindole) positive bands located in all telomeric regions of this species did not hybridize with either Z. perennis or Z diploperennis genomic probe. Therefore, Z. luxurians has a repetitive sequence that can be used in fluorescent staining to identify its chromosomes. When GISH was employed on metaphase I of the 2n = 30 hybrid, all the univalents showed distinctive telomeres of Z. luxurians, while the bivalents did not present any signal. These findings show that the formation of bivalent-univalent configurations is not a random event. The bivalents tend to be spatially separated and are very often observed forming an independent group of 5II. Finally, trivalents were composed by one chromosome labeled in its telomeric regions, and two smaller and unlabeled ones. The use of chromosome markers of Z. luxurians demonstrated to be a good step forward in interpreting the nature of meiotic configurations in 2n = 30 Zea spp. hybrids. They can help to clarify the relationship between genomes and provide a useful addition to the taxonomic classification in the genus Zea. |
| author |
Poggio, Lidia Confalonieri, Viviana Andrea Comas, Cecilia Isabel González, Graciela Esther Naranjo, Carlos Alberto |
| author_facet |
Poggio, Lidia Confalonieri, Viviana Andrea Comas, Cecilia Isabel González, Graciela Esther Naranjo, Carlos Alberto |
| author_sort |
Poggio, Lidia |
| title |
Genomic affinities of Zea luxurians, Z. diploperennis, And Z. perennis: Meiotic behavior of their F1 hybrids and genomic in situ hybridization (GISH) |
| title_short |
Genomic affinities of Zea luxurians, Z. diploperennis, And Z. perennis: Meiotic behavior of their F1 hybrids and genomic in situ hybridization (GISH) |
| title_full |
Genomic affinities of Zea luxurians, Z. diploperennis, And Z. perennis: Meiotic behavior of their F1 hybrids and genomic in situ hybridization (GISH) |
| title_fullStr |
Genomic affinities of Zea luxurians, Z. diploperennis, And Z. perennis: Meiotic behavior of their F1 hybrids and genomic in situ hybridization (GISH) |
| title_full_unstemmed |
Genomic affinities of Zea luxurians, Z. diploperennis, And Z. perennis: Meiotic behavior of their F1 hybrids and genomic in situ hybridization (GISH) |
| title_sort |
genomic affinities of zea luxurians, z. diploperennis, and z. perennis: meiotic behavior of their f1 hybrids and genomic in situ hybridization (gish) |
| publishDate |
1999 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v42_n5_p993_Poggio http://hdl.handle.net/20.500.12110/paper_08312796_v42_n5_p993_Poggio |
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| _version_ |
1768542087830044672 |