Conjugal transfer of bacteriophage resistance to prolate phages in Lactococcus lactis

Conjugal transfer of lactose-fermenting ability (Lac+) and lytic phage resistance (Lpr+) was demonstrated in four matings between Lactococcus lactis ssp. lactis C2, phage resistant mutant donor strains C21, C23 and C26 and the C2 derivative LM2302 plasmid free recipient strain. Transconjugants were...

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Publicado: 1992
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07400020_v9_n3_p251_DeFabrizio
http://hdl.handle.net/20.500.12110/paper_07400020_v9_n3_p251_DeFabrizio
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_07400020_v9_n3_p251_DeFabrizio
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spelling paper:paper_07400020_v9_n3_p251_DeFabrizio2025-07-30T18:20:59Z Conjugal transfer of bacteriophage resistance to prolate phages in Lactococcus lactis Conjugal transfer of lactose-fermenting ability (Lac+) and lytic phage resistance (Lpr+) was demonstrated in four matings between Lactococcus lactis ssp. lactis C2, phage resistant mutant donor strains C21, C23 and C26 and the C2 derivative LM2302 plasmid free recipient strain. Transconjugants were selected with Lac+ Emr and/or Str phenotypes. Lac+ was transferred from donor strains to Lac- Emr and r Str phage sensitive recipient strains at a frequency of 10-6 to 10-8 revealing that Lac+ and subsequently Lpr+ were transferred. Although clumping (Clu) effects with a subsequent high frequency transconjugation (Hft) have been observed before in related strains, it was not evident in this case. Phage assays with Argentine (ARG) and United States (US) phage revealed that recipient strains were Lpr-. Because the transconjugants had the same lytic pattern as the corresponding donor strain, it is apparent that the unselected Lpr+ trait was cotransferred with the Lac+ marker on a 45 kb plasmid. A significant reduction in bacteriophage adsorption and failures in plaque formations for the ARG and the US prolate phages in all Lpr+ strains was also observed. These results suggest that the Lpr+ trait is mobilized by conjugal transfer. © 1992 Academic Press Limited. 1992 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07400020_v9_n3_p251_DeFabrizio http://hdl.handle.net/20.500.12110/paper_07400020_v9_n3_p251_DeFabrizio
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description Conjugal transfer of lactose-fermenting ability (Lac+) and lytic phage resistance (Lpr+) was demonstrated in four matings between Lactococcus lactis ssp. lactis C2, phage resistant mutant donor strains C21, C23 and C26 and the C2 derivative LM2302 plasmid free recipient strain. Transconjugants were selected with Lac+ Emr and/or Str phenotypes. Lac+ was transferred from donor strains to Lac- Emr and r Str phage sensitive recipient strains at a frequency of 10-6 to 10-8 revealing that Lac+ and subsequently Lpr+ were transferred. Although clumping (Clu) effects with a subsequent high frequency transconjugation (Hft) have been observed before in related strains, it was not evident in this case. Phage assays with Argentine (ARG) and United States (US) phage revealed that recipient strains were Lpr-. Because the transconjugants had the same lytic pattern as the corresponding donor strain, it is apparent that the unselected Lpr+ trait was cotransferred with the Lac+ marker on a 45 kb plasmid. A significant reduction in bacteriophage adsorption and failures in plaque formations for the ARG and the US prolate phages in all Lpr+ strains was also observed. These results suggest that the Lpr+ trait is mobilized by conjugal transfer. © 1992 Academic Press Limited.
title Conjugal transfer of bacteriophage resistance to prolate phages in Lactococcus lactis
spellingShingle Conjugal transfer of bacteriophage resistance to prolate phages in Lactococcus lactis
title_short Conjugal transfer of bacteriophage resistance to prolate phages in Lactococcus lactis
title_full Conjugal transfer of bacteriophage resistance to prolate phages in Lactococcus lactis
title_fullStr Conjugal transfer of bacteriophage resistance to prolate phages in Lactococcus lactis
title_full_unstemmed Conjugal transfer of bacteriophage resistance to prolate phages in Lactococcus lactis
title_sort conjugal transfer of bacteriophage resistance to prolate phages in lactococcus lactis
publishDate 1992
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07400020_v9_n3_p251_DeFabrizio
http://hdl.handle.net/20.500.12110/paper_07400020_v9_n3_p251_DeFabrizio
_version_ 1840327738240532480

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