Nuclear DNA content in the polyploid complex Turnera ulmifolia (Turnera L., Passifloraceae)

Turnera ulmifolia constitutes a well-studied polyploid complex with allo- and autopolyploid species ranging from 2 to 8x. Flow cytometry was used to determine nuclear DNA content, and to estimate 2C- and 1Cx-values with the aim of analysing the genome size in Turnera in terms of polyploid speciation...

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Autores principales:	López, Alicia, Poggio, Lidia
Publicado:	2011
Materias:	2C-value Cx-value DNA content Passifloraceae Polyploid complex Turnera Turneraceae Passiflora edulis Turnera aurelii Turnera cuneiformis Turnera grandidentata Turnera ulmifolia
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03782697_v296_n3-4_p225_Lopez http://hdl.handle.net/20.500.12110/paper_03782697_v296_n3-4_p225_Lopez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03782697_v296_n3-4_p225_Lopez
record_format	dspace
spelling	paper:paper_03782697_v296_n3-4_p225_Lopez2023-06-08T15:39:34Z Nuclear DNA content in the polyploid complex Turnera ulmifolia (Turnera L., Passifloraceae) López, Alicia Poggio, Lidia 2C-value Cx-value DNA content Passifloraceae Polyploid complex Turnera Turneraceae Passiflora edulis Passifloraceae Turnera Turnera aurelii Turnera cuneiformis Turnera grandidentata Turnera ulmifolia Turneraceae Turnera ulmifolia constitutes a well-studied polyploid complex with allo- and autopolyploid species ranging from 2 to 8x. Flow cytometry was used to determine nuclear DNA content, and to estimate 2C- and 1Cx-values with the aim of analysing the genome size in Turnera in terms of polyploid speciation. The 2C-value and 1Cx-value were evaluated in 12 species of the T. ulmifolia complex. Nuclear DNA content was estimated by flow cytometry of nuclei stained with propidium iodide. The 2C DNA content ranged from 1.38 to 1.83 pg in diploids, from 2.67 to 3.96 pg in tetraploids, from 2.73 to 4.31 pg in hexaploids, and from 3.53 to 5.90 pg in octoploids. The 1Cx-value ranged from 0.44 to 0.99 pg. The Turnera ulmifolia complex showed an increase in total DNA content in the ploidy level, but not in the expected proportion. The general tendency indicated a decrease in the 1Cx-value with increasing chromosome number, with T. grandidentata 4x being an outstanding exception. The 1Cx-values in the allooctoploids T. aurelii and T. cuneiformis differed by 1.6-fold from each other, probably as a result of different evolutionary histories following divergence from the last common ancestor. © 2011 Springer-Verlag. Fil:López, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Poggio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03782697_v296_n3-4_p225_Lopez http://hdl.handle.net/20.500.12110/paper_03782697_v296_n3-4_p225_Lopez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	2C-value Cx-value DNA content Passifloraceae Polyploid complex Turnera Turneraceae Passiflora edulis Passifloraceae Turnera Turnera aurelii Turnera cuneiformis Turnera grandidentata Turnera ulmifolia Turneraceae
spellingShingle	2C-value Cx-value DNA content Passifloraceae Polyploid complex Turnera Turneraceae Passiflora edulis Passifloraceae Turnera Turnera aurelii Turnera cuneiformis Turnera grandidentata Turnera ulmifolia Turneraceae López, Alicia Poggio, Lidia Nuclear DNA content in the polyploid complex Turnera ulmifolia (Turnera L., Passifloraceae)
topic_facet	2C-value Cx-value DNA content Passifloraceae Polyploid complex Turnera Turneraceae Passiflora edulis Passifloraceae Turnera Turnera aurelii Turnera cuneiformis Turnera grandidentata Turnera ulmifolia Turneraceae
description	Turnera ulmifolia constitutes a well-studied polyploid complex with allo- and autopolyploid species ranging from 2 to 8x. Flow cytometry was used to determine nuclear DNA content, and to estimate 2C- and 1Cx-values with the aim of analysing the genome size in Turnera in terms of polyploid speciation. The 2C-value and 1Cx-value were evaluated in 12 species of the T. ulmifolia complex. Nuclear DNA content was estimated by flow cytometry of nuclei stained with propidium iodide. The 2C DNA content ranged from 1.38 to 1.83 pg in diploids, from 2.67 to 3.96 pg in tetraploids, from 2.73 to 4.31 pg in hexaploids, and from 3.53 to 5.90 pg in octoploids. The 1Cx-value ranged from 0.44 to 0.99 pg. The Turnera ulmifolia complex showed an increase in total DNA content in the ploidy level, but not in the expected proportion. The general tendency indicated a decrease in the 1Cx-value with increasing chromosome number, with T. grandidentata 4x being an outstanding exception. The 1Cx-values in the allooctoploids T. aurelii and T. cuneiformis differed by 1.6-fold from each other, probably as a result of different evolutionary histories following divergence from the last common ancestor. © 2011 Springer-Verlag.
author	López, Alicia Poggio, Lidia
author_facet	López, Alicia Poggio, Lidia
author_sort	López, Alicia
title	Nuclear DNA content in the polyploid complex Turnera ulmifolia (Turnera L., Passifloraceae)
title_short	Nuclear DNA content in the polyploid complex Turnera ulmifolia (Turnera L., Passifloraceae)
title_full	Nuclear DNA content in the polyploid complex Turnera ulmifolia (Turnera L., Passifloraceae)
title_fullStr	Nuclear DNA content in the polyploid complex Turnera ulmifolia (Turnera L., Passifloraceae)
title_full_unstemmed	Nuclear DNA content in the polyploid complex Turnera ulmifolia (Turnera L., Passifloraceae)
title_sort	nuclear dna content in the polyploid complex turnera ulmifolia (turnera l., passifloraceae)
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03782697_v296_n3-4_p225_Lopez http://hdl.handle.net/20.500.12110/paper_03782697_v296_n3-4_p225_Lopez
work_keys_str_mv	AT lopezalicia nucleardnacontentinthepolyploidcomplexturneraulmifoliaturneralpassifloraceae AT poggiolidia nucleardnacontentinthepolyploidcomplexturneraulmifoliaturneralpassifloraceae
_version_	1768543374056357888

Nuclear DNA content in the polyploid complex Turnera ulmifolia (Turnera L., Passifloraceae)

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