Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species

Exposure of either gametes or embryos to conditions and/or factors that generate oxidative stress has been associated with impaired early embryogenesis. The effects of reactive oxygen species (ROS) on mouse preimplantation development, depending of the ROS-concentration and time of exposition, were...

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Autor principal:	Cebral, Elisa
Publicado:	2007
Materias:	Mouse Preimplantation embryogenesis Reactive oxygen species Toxicity hydrogen peroxide reactive oxygen metabolite animal experiment animal model article cell cycle arrest controlled study culture medium embryo embryo development embryotoxicity female male mouse nonhuman oxidative stress preimplantation embryo Animals Blastocyst Cleavage Stage, Ovum Embryo Transfer Embryonic Development Female Hydrogen Peroxide Male Mice Oxidative Stress Reactive Oxygen Species
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v31_n1_p51_Cebral http://hdl.handle.net/20.500.12110/paper_03279545_v31_n1_p51_Cebral
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03279545_v31_n1_p51_Cebral
record_format	dspace
spelling	paper:paper_03279545_v31_n1_p51_Cebral2023-06-08T15:33:42Z Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species Cebral, Elisa Mouse Preimplantation embryogenesis Reactive oxygen species Toxicity hydrogen peroxide reactive oxygen metabolite animal experiment animal model article cell cycle arrest controlled study culture medium embryo embryo development embryotoxicity female male mouse nonhuman oxidative stress preimplantation embryo Animals Blastocyst Cleavage Stage, Ovum Embryo Transfer Embryonic Development Female Hydrogen Peroxide Male Mice Oxidative Stress Reactive Oxygen Species Exposure of either gametes or embryos to conditions and/or factors that generate oxidative stress has been associated with impaired early embryogenesis. The effects of reactive oxygen species (ROS) on mouse preimplantation development, depending of the ROS-concentration and time of exposition, were studied. Two-cell embryos were incubated with 5, 10, 25 and 50 μM of hydrogen peroxide (H2O2) for 30 and 60 minutes of exposition and allowed to develop for 72 h to study the quality of development. The incubation with 50 μM H2O2 for 30 or 60 minutes, strongly inhibited the 2-cell embryo development as compared to the control (p<0.001). Twenty-five μM H2O2 produced inhibition of blastocyst formation (p<0.001) and 10 μM H2O2 significantly decreased the percentages of expanded and hatched blastocysts, which resulted morphologically altered (p<0.05 and p<0.01, respectively). The higher H2O2 concentrations were able to elicit necrotic morphology in the 2-cell arrested embryos, while 10 μM H2O 2 induced moderate damage with the arrested embryos partially fragmented. In conclusion, important causes for defective preimplantation development and for early embryo losses may be due to oxidative stress because early mouse embryos exposed to ROS for short times arrested at the first cellular cycle (2-cell) and/or impaired embryo differentiation and morphogenesis, being these effects ROS-concentration-dependent. Fil:Cebral, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v31_n1_p51_Cebral http://hdl.handle.net/20.500.12110/paper_03279545_v31_n1_p51_Cebral
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Mouse Preimplantation embryogenesis Reactive oxygen species Toxicity hydrogen peroxide reactive oxygen metabolite animal experiment animal model article cell cycle arrest controlled study culture medium embryo embryo development embryotoxicity female male mouse nonhuman oxidative stress preimplantation embryo Animals Blastocyst Cleavage Stage, Ovum Embryo Transfer Embryonic Development Female Hydrogen Peroxide Male Mice Oxidative Stress Reactive Oxygen Species
spellingShingle	Mouse Preimplantation embryogenesis Reactive oxygen species Toxicity hydrogen peroxide reactive oxygen metabolite animal experiment animal model article cell cycle arrest controlled study culture medium embryo embryo development embryotoxicity female male mouse nonhuman oxidative stress preimplantation embryo Animals Blastocyst Cleavage Stage, Ovum Embryo Transfer Embryonic Development Female Hydrogen Peroxide Male Mice Oxidative Stress Reactive Oxygen Species Cebral, Elisa Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species
topic_facet	Mouse Preimplantation embryogenesis Reactive oxygen species Toxicity hydrogen peroxide reactive oxygen metabolite animal experiment animal model article cell cycle arrest controlled study culture medium embryo embryo development embryotoxicity female male mouse nonhuman oxidative stress preimplantation embryo Animals Blastocyst Cleavage Stage, Ovum Embryo Transfer Embryonic Development Female Hydrogen Peroxide Male Mice Oxidative Stress Reactive Oxygen Species
description	Exposure of either gametes or embryos to conditions and/or factors that generate oxidative stress has been associated with impaired early embryogenesis. The effects of reactive oxygen species (ROS) on mouse preimplantation development, depending of the ROS-concentration and time of exposition, were studied. Two-cell embryos were incubated with 5, 10, 25 and 50 μM of hydrogen peroxide (H2O2) for 30 and 60 minutes of exposition and allowed to develop for 72 h to study the quality of development. The incubation with 50 μM H2O2 for 30 or 60 minutes, strongly inhibited the 2-cell embryo development as compared to the control (p<0.001). Twenty-five μM H2O2 produced inhibition of blastocyst formation (p<0.001) and 10 μM H2O2 significantly decreased the percentages of expanded and hatched blastocysts, which resulted morphologically altered (p<0.05 and p<0.01, respectively). The higher H2O2 concentrations were able to elicit necrotic morphology in the 2-cell arrested embryos, while 10 μM H2O 2 induced moderate damage with the arrested embryos partially fragmented. In conclusion, important causes for defective preimplantation development and for early embryo losses may be due to oxidative stress because early mouse embryos exposed to ROS for short times arrested at the first cellular cycle (2-cell) and/or impaired embryo differentiation and morphogenesis, being these effects ROS-concentration-dependent.
author	Cebral, Elisa
author_facet	Cebral, Elisa
author_sort	Cebral, Elisa
title	Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species
title_short	Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species
title_full	Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species
title_fullStr	Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species
title_full_unstemmed	Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species
title_sort	preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species
publishDate	2007
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v31_n1_p51_Cebral http://hdl.handle.net/20.500.12110/paper_03279545_v31_n1_p51_Cebral
work_keys_str_mv	AT cebralelisa preimplantationembryotoxicityaftermouseembryoexpositiontoreactiveoxygenspecies
_version_	1768545976753061888

Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species

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