Sequencing of the bicistronic genome segments S7 and S9 of Mal de Río Cuarto virus (Fijivirus, Reoviridae) completes the genome of this virus
The nucleotide sequences of genomic segments S7 and S9 of Mal de Río Cuarto virus (MRCV, Fijivirus group II) have been determined, thus completing the entire genome sequence of the virus. These segments showed a non-overlapping bicistronic structure, as in other members of the genus. MRCV S7 ORF-1 h...
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2007
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v152_n3_p565_Guzman http://hdl.handle.net/20.500.12110/paper_03048608_v152_n3_p565_Guzman |
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paper:paper_03048608_v152_n3_p565_Guzman2023-06-08T15:30:05Z Sequencing of the bicistronic genome segments S7 and S9 of Mal de Río Cuarto virus (Fijivirus, Reoviridae) completes the genome of this virus Distéfano, Ana Julia Hopp, Horacio Esteban Del Vas, Mariana 3' untranslated region 5' untranslated region article biology classification genetics molecular cloning nucleotide sequence phylogeny Reovirus sequence homology virus genome 3' Untranslated Regions 5' Untranslated Regions Base Sequence Cloning, Molecular Computational Biology Genome, Viral Phylogeny Reoviridae Sequence Homology, Nucleic Acid Fijivirus Maize rough dwarf virus Mal de Rio Cuarto virus Orthoreovirus Reoviridae Rice black streaked dwarf virus Zea mays The nucleotide sequences of genomic segments S7 and S9 of Mal de Río Cuarto virus (MRCV, Fijivirus group II) have been determined, thus completing the entire genome sequence of the virus. These segments showed a non-overlapping bicistronic structure, as in other members of the genus. MRCV S7 ORF-1 had a length of 1086 bp and encoded a 41.5 kDa putative polypeptide, whereas MRCV S7 ORF-2 had a length of 930 bp and encoded a 36.8 kDa putative polypeptide. Proteins of 39 and 20.5 kDa were predicted for the 1014 bp long MRCV S9 ORF-1 and the 537 bp long MRCV S9 ORF-2, respectively. The terminal 5′ and 3′ sequences of both segments were 5′AAGUUUUU3′ and 5′CAGCUnnnGUC3′, respectively. Specific imperfect inverted repeats of each segment were identified. Comparison of the predicted proteins with those of related virus genome segments counterparts in maize rough dwarf virus (MRDV) and rice black streaked dwarf virus (RBSDV), showed 64.5-44.3% identities. These values are lower than those resulting from comparisons between MRDV and RBSDV. The topology of the trees obtained using the complete nucleotide and amino acid sequences of MRCV S7 and MRCV S9 was consistent with the analysis of the other MRCV segments previously published. © 2006 Springer-Verlag. Fil:Distéfano, A.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del Vas, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v152_n3_p565_Guzman http://hdl.handle.net/20.500.12110/paper_03048608_v152_n3_p565_Guzman |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
3' untranslated region 5' untranslated region article biology classification genetics molecular cloning nucleotide sequence phylogeny Reovirus sequence homology virus genome 3' Untranslated Regions 5' Untranslated Regions Base Sequence Cloning, Molecular Computational Biology Genome, Viral Phylogeny Reoviridae Sequence Homology, Nucleic Acid Fijivirus Maize rough dwarf virus Mal de Rio Cuarto virus Orthoreovirus Reoviridae Rice black streaked dwarf virus Zea mays |
| spellingShingle |
3' untranslated region 5' untranslated region article biology classification genetics molecular cloning nucleotide sequence phylogeny Reovirus sequence homology virus genome 3' Untranslated Regions 5' Untranslated Regions Base Sequence Cloning, Molecular Computational Biology Genome, Viral Phylogeny Reoviridae Sequence Homology, Nucleic Acid Fijivirus Maize rough dwarf virus Mal de Rio Cuarto virus Orthoreovirus Reoviridae Rice black streaked dwarf virus Zea mays Distéfano, Ana Julia Hopp, Horacio Esteban Del Vas, Mariana Sequencing of the bicistronic genome segments S7 and S9 of Mal de Río Cuarto virus (Fijivirus, Reoviridae) completes the genome of this virus |
| topic_facet |
3' untranslated region 5' untranslated region article biology classification genetics molecular cloning nucleotide sequence phylogeny Reovirus sequence homology virus genome 3' Untranslated Regions 5' Untranslated Regions Base Sequence Cloning, Molecular Computational Biology Genome, Viral Phylogeny Reoviridae Sequence Homology, Nucleic Acid Fijivirus Maize rough dwarf virus Mal de Rio Cuarto virus Orthoreovirus Reoviridae Rice black streaked dwarf virus Zea mays |
| description |
The nucleotide sequences of genomic segments S7 and S9 of Mal de Río Cuarto virus (MRCV, Fijivirus group II) have been determined, thus completing the entire genome sequence of the virus. These segments showed a non-overlapping bicistronic structure, as in other members of the genus. MRCV S7 ORF-1 had a length of 1086 bp and encoded a 41.5 kDa putative polypeptide, whereas MRCV S7 ORF-2 had a length of 930 bp and encoded a 36.8 kDa putative polypeptide. Proteins of 39 and 20.5 kDa were predicted for the 1014 bp long MRCV S9 ORF-1 and the 537 bp long MRCV S9 ORF-2, respectively. The terminal 5′ and 3′ sequences of both segments were 5′AAGUUUUU3′ and 5′CAGCUnnnGUC3′, respectively. Specific imperfect inverted repeats of each segment were identified. Comparison of the predicted proteins with those of related virus genome segments counterparts in maize rough dwarf virus (MRDV) and rice black streaked dwarf virus (RBSDV), showed 64.5-44.3% identities. These values are lower than those resulting from comparisons between MRDV and RBSDV. The topology of the trees obtained using the complete nucleotide and amino acid sequences of MRCV S7 and MRCV S9 was consistent with the analysis of the other MRCV segments previously published. © 2006 Springer-Verlag. |
| author |
Distéfano, Ana Julia Hopp, Horacio Esteban Del Vas, Mariana |
| author_facet |
Distéfano, Ana Julia Hopp, Horacio Esteban Del Vas, Mariana |
| author_sort |
Distéfano, Ana Julia |
| title |
Sequencing of the bicistronic genome segments S7 and S9 of Mal de Río Cuarto virus (Fijivirus, Reoviridae) completes the genome of this virus |
| title_short |
Sequencing of the bicistronic genome segments S7 and S9 of Mal de Río Cuarto virus (Fijivirus, Reoviridae) completes the genome of this virus |
| title_full |
Sequencing of the bicistronic genome segments S7 and S9 of Mal de Río Cuarto virus (Fijivirus, Reoviridae) completes the genome of this virus |
| title_fullStr |
Sequencing of the bicistronic genome segments S7 and S9 of Mal de Río Cuarto virus (Fijivirus, Reoviridae) completes the genome of this virus |
| title_full_unstemmed |
Sequencing of the bicistronic genome segments S7 and S9 of Mal de Río Cuarto virus (Fijivirus, Reoviridae) completes the genome of this virus |
| title_sort |
sequencing of the bicistronic genome segments s7 and s9 of mal de río cuarto virus (fijivirus, reoviridae) completes the genome of this virus |
| publishDate |
2007 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v152_n3_p565_Guzman http://hdl.handle.net/20.500.12110/paper_03048608_v152_n3_p565_Guzman |
| work_keys_str_mv |
AT distefanoanajulia sequencingofthebicistronicgenomesegmentss7ands9ofmalderiocuartovirusfijivirusreoviridaecompletesthegenomeofthisvirus AT hopphoracioesteban sequencingofthebicistronicgenomesegmentss7ands9ofmalderiocuartovirusfijivirusreoviridaecompletesthegenomeofthisvirus AT delvasmariana sequencingofthebicistronicgenomesegmentss7ands9ofmalderiocuartovirusfijivirusreoviridaecompletesthegenomeofthisvirus |
| _version_ |
1768546160350330880 |