H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1

The aim of the present study was to evaluate cell cycle regulation by scavenging H2O2 in tumor cells. A significant arrest in the G1 phase of the cell cycle was demonstrated in CH72-T4 carcinoma cells exposed to catalase, associated with a decrease in cyclin D1 and an increase in the CDK inhibitory...

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Autores principales: Policastro, Lucía Laura, Palmieri, Mónica Alejandra, Molinari de Rey, Beatriz, Durán, Hebe Alicia
Publicado: 2011
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043835_v305_n1_p58_Ibanez
http://hdl.handle.net/20.500.12110/paper_03043835_v305_n1_p58_Ibanez
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spelling paper:paper_03043835_v305_n1_p58_Ibanez2023-06-08T15:29:18Z H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1 Policastro, Lucía Laura Palmieri, Mónica Alejandra Molinari de Rey, Beatriz Durán, Hebe Alicia Cancer cells Cell cycle Cyclin D1 Hydrogen peroxide P27kip1 Proliferation catalase cyclin D1 cyclin dependent kinase inhibitor 1B hydrogen peroxide catalase cyclin D1 cyclin dependent kinase inhibitor 1B hydrogen peroxide reactive oxygen metabolite scavenger animal cell animal experiment animal model animal tissue antineoplastic activity article cancer inhibition carcinoma cell cell cycle arrest cell cycle G1 phase cell cycle regulation cell cycle S phase cell level cell nucleus cell proliferation cellular distribution controlled study cytoplasm female in vitro study in vivo study intracellular space mouse nonhuman priority journal protein analysis protein localization protein modification squamous cell carcinoma active transport animal drug effect fluorescent antibody technique metabolism nude mouse sensitive to carcinogenesis mouse tumor cell line Western blotting Active Transport, Cell Nucleus Animals Blotting, Western Catalase Cell Line, Tumor Cell Nucleus Cyclin D1 Cyclin-Dependent Kinase Inhibitor p27 Female Fluorescent Antibody Technique Free Radical Scavengers G1 Phase Hydrogen Peroxide Mice Mice, Inbred SENCAR Mice, Nude Reactive Oxygen Species S Phase The aim of the present study was to evaluate cell cycle regulation by scavenging H2O2 in tumor cells. A significant arrest in the G1 phase of the cell cycle was demonstrated in CH72-T4 carcinoma cells exposed to catalase, associated with a decrease in cyclin D1 and an increase in the CDK inhibitory protein p27KIP1. Moreover, we found a differential intracellular distribution of p27KIP1, which remained in the nucleus after catalase treatment. In vivo experiments showed an increase in nuclear levels of p27KIP1 associated with the inhibition of tumor growth by H2O2 scavenging, confirming in vitro results. To conclude, H2O2 scavenging may induce cell cycle arrest through the modulation of cyclin D1 and p27KIP1 levels and nuclear localization of p27KIP1. To our knowledge, this is the first report that demonstrates that the modulation of ROS alters the intracellular localization of a key regulatory protein of G1/S transition. © 2011 Elsevier Ireland Ltd. Fil:Policastro, L.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Palmieri, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Molinari, B.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Durán, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043835_v305_n1_p58_Ibanez http://hdl.handle.net/20.500.12110/paper_03043835_v305_n1_p58_Ibanez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Cancer cells
Cell cycle
Cyclin D1
Hydrogen peroxide
P27kip1
Proliferation
catalase
cyclin D1
cyclin dependent kinase inhibitor 1B
hydrogen peroxide
catalase
cyclin D1
cyclin dependent kinase inhibitor 1B
hydrogen peroxide
reactive oxygen metabolite
scavenger
animal cell
animal experiment
animal model
animal tissue
antineoplastic activity
article
cancer inhibition
carcinoma cell
cell cycle arrest
cell cycle G1 phase
cell cycle regulation
cell cycle S phase
cell level
cell nucleus
cell proliferation
cellular distribution
controlled study
cytoplasm
female
in vitro study
in vivo study
intracellular space
mouse
nonhuman
priority journal
protein analysis
protein localization
protein modification
squamous cell carcinoma
active transport
animal
drug effect
fluorescent antibody technique
metabolism
nude mouse
sensitive to carcinogenesis mouse
tumor cell line
Western blotting
Active Transport, Cell Nucleus
Animals
Blotting, Western
Catalase
Cell Line, Tumor
Cell Nucleus
Cyclin D1
Cyclin-Dependent Kinase Inhibitor p27
Female
Fluorescent Antibody Technique
Free Radical Scavengers
G1 Phase
Hydrogen Peroxide
Mice
Mice, Inbred SENCAR
Mice, Nude
Reactive Oxygen Species
S Phase
spellingShingle Cancer cells
Cell cycle
Cyclin D1
Hydrogen peroxide
P27kip1
Proliferation
catalase
cyclin D1
cyclin dependent kinase inhibitor 1B
hydrogen peroxide
catalase
cyclin D1
cyclin dependent kinase inhibitor 1B
hydrogen peroxide
reactive oxygen metabolite
scavenger
animal cell
animal experiment
animal model
animal tissue
antineoplastic activity
article
cancer inhibition
carcinoma cell
cell cycle arrest
cell cycle G1 phase
cell cycle regulation
cell cycle S phase
cell level
cell nucleus
cell proliferation
cellular distribution
controlled study
cytoplasm
female
in vitro study
in vivo study
intracellular space
mouse
nonhuman
priority journal
protein analysis
protein localization
protein modification
squamous cell carcinoma
active transport
animal
drug effect
fluorescent antibody technique
metabolism
nude mouse
sensitive to carcinogenesis mouse
tumor cell line
Western blotting
Active Transport, Cell Nucleus
Animals
Blotting, Western
Catalase
Cell Line, Tumor
Cell Nucleus
Cyclin D1
Cyclin-Dependent Kinase Inhibitor p27
Female
Fluorescent Antibody Technique
Free Radical Scavengers
G1 Phase
Hydrogen Peroxide
Mice
Mice, Inbred SENCAR
Mice, Nude
Reactive Oxygen Species
S Phase
Policastro, Lucía Laura
Palmieri, Mónica Alejandra
Molinari de Rey, Beatriz
Durán, Hebe Alicia
H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1
topic_facet Cancer cells
Cell cycle
Cyclin D1
Hydrogen peroxide
P27kip1
Proliferation
catalase
cyclin D1
cyclin dependent kinase inhibitor 1B
hydrogen peroxide
catalase
cyclin D1
cyclin dependent kinase inhibitor 1B
hydrogen peroxide
reactive oxygen metabolite
scavenger
animal cell
animal experiment
animal model
animal tissue
antineoplastic activity
article
cancer inhibition
carcinoma cell
cell cycle arrest
cell cycle G1 phase
cell cycle regulation
cell cycle S phase
cell level
cell nucleus
cell proliferation
cellular distribution
controlled study
cytoplasm
female
in vitro study
in vivo study
intracellular space
mouse
nonhuman
priority journal
protein analysis
protein localization
protein modification
squamous cell carcinoma
active transport
animal
drug effect
fluorescent antibody technique
metabolism
nude mouse
sensitive to carcinogenesis mouse
tumor cell line
Western blotting
Active Transport, Cell Nucleus
Animals
Blotting, Western
Catalase
Cell Line, Tumor
Cell Nucleus
Cyclin D1
Cyclin-Dependent Kinase Inhibitor p27
Female
Fluorescent Antibody Technique
Free Radical Scavengers
G1 Phase
Hydrogen Peroxide
Mice
Mice, Inbred SENCAR
Mice, Nude
Reactive Oxygen Species
S Phase
description The aim of the present study was to evaluate cell cycle regulation by scavenging H2O2 in tumor cells. A significant arrest in the G1 phase of the cell cycle was demonstrated in CH72-T4 carcinoma cells exposed to catalase, associated with a decrease in cyclin D1 and an increase in the CDK inhibitory protein p27KIP1. Moreover, we found a differential intracellular distribution of p27KIP1, which remained in the nucleus after catalase treatment. In vivo experiments showed an increase in nuclear levels of p27KIP1 associated with the inhibition of tumor growth by H2O2 scavenging, confirming in vitro results. To conclude, H2O2 scavenging may induce cell cycle arrest through the modulation of cyclin D1 and p27KIP1 levels and nuclear localization of p27KIP1. To our knowledge, this is the first report that demonstrates that the modulation of ROS alters the intracellular localization of a key regulatory protein of G1/S transition. © 2011 Elsevier Ireland Ltd.
author Policastro, Lucía Laura
Palmieri, Mónica Alejandra
Molinari de Rey, Beatriz
Durán, Hebe Alicia
author_facet Policastro, Lucía Laura
Palmieri, Mónica Alejandra
Molinari de Rey, Beatriz
Durán, Hebe Alicia
author_sort Policastro, Lucía Laura
title H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1
title_short H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1
title_full H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1
title_fullStr H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1
title_full_unstemmed H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1
title_sort h2o2 scavenging inhibits g1/s transition by increasing nuclear levels of p27kip1
publishDate 2011
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043835_v305_n1_p58_Ibanez
http://hdl.handle.net/20.500.12110/paper_03043835_v305_n1_p58_Ibanez
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AT palmierimonicaalejandra h2o2scavenginginhibitsg1stransitionbyincreasingnuclearlevelsofp27kip1
AT molinaridereybeatriz h2o2scavenginginhibitsg1stransitionbyincreasingnuclearlevelsofp27kip1
AT duranhebealicia h2o2scavenginginhibitsg1stransitionbyincreasingnuclearlevelsofp27kip1
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