H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1
The aim of the present study was to evaluate cell cycle regulation by scavenging H2O2 in tumor cells. A significant arrest in the G1 phase of the cell cycle was demonstrated in CH72-T4 carcinoma cells exposed to catalase, associated with a decrease in cyclin D1 and an increase in the CDK inhibitory...
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2011
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043835_v305_n1_p58_Ibanez http://hdl.handle.net/20.500.12110/paper_03043835_v305_n1_p58_Ibanez |
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paper:paper_03043835_v305_n1_p58_Ibanez2023-06-08T15:29:18Z H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1 Policastro, Lucía Laura Palmieri, Mónica Alejandra Molinari de Rey, Beatriz Durán, Hebe Alicia Cancer cells Cell cycle Cyclin D1 Hydrogen peroxide P27kip1 Proliferation catalase cyclin D1 cyclin dependent kinase inhibitor 1B hydrogen peroxide catalase cyclin D1 cyclin dependent kinase inhibitor 1B hydrogen peroxide reactive oxygen metabolite scavenger animal cell animal experiment animal model animal tissue antineoplastic activity article cancer inhibition carcinoma cell cell cycle arrest cell cycle G1 phase cell cycle regulation cell cycle S phase cell level cell nucleus cell proliferation cellular distribution controlled study cytoplasm female in vitro study in vivo study intracellular space mouse nonhuman priority journal protein analysis protein localization protein modification squamous cell carcinoma active transport animal drug effect fluorescent antibody technique metabolism nude mouse sensitive to carcinogenesis mouse tumor cell line Western blotting Active Transport, Cell Nucleus Animals Blotting, Western Catalase Cell Line, Tumor Cell Nucleus Cyclin D1 Cyclin-Dependent Kinase Inhibitor p27 Female Fluorescent Antibody Technique Free Radical Scavengers G1 Phase Hydrogen Peroxide Mice Mice, Inbred SENCAR Mice, Nude Reactive Oxygen Species S Phase The aim of the present study was to evaluate cell cycle regulation by scavenging H2O2 in tumor cells. A significant arrest in the G1 phase of the cell cycle was demonstrated in CH72-T4 carcinoma cells exposed to catalase, associated with a decrease in cyclin D1 and an increase in the CDK inhibitory protein p27KIP1. Moreover, we found a differential intracellular distribution of p27KIP1, which remained in the nucleus after catalase treatment. In vivo experiments showed an increase in nuclear levels of p27KIP1 associated with the inhibition of tumor growth by H2O2 scavenging, confirming in vitro results. To conclude, H2O2 scavenging may induce cell cycle arrest through the modulation of cyclin D1 and p27KIP1 levels and nuclear localization of p27KIP1. To our knowledge, this is the first report that demonstrates that the modulation of ROS alters the intracellular localization of a key regulatory protein of G1/S transition. © 2011 Elsevier Ireland Ltd. Fil:Policastro, L.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Palmieri, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Molinari, B.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Durán, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043835_v305_n1_p58_Ibanez http://hdl.handle.net/20.500.12110/paper_03043835_v305_n1_p58_Ibanez |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Cancer cells Cell cycle Cyclin D1 Hydrogen peroxide P27kip1 Proliferation catalase cyclin D1 cyclin dependent kinase inhibitor 1B hydrogen peroxide catalase cyclin D1 cyclin dependent kinase inhibitor 1B hydrogen peroxide reactive oxygen metabolite scavenger animal cell animal experiment animal model animal tissue antineoplastic activity article cancer inhibition carcinoma cell cell cycle arrest cell cycle G1 phase cell cycle regulation cell cycle S phase cell level cell nucleus cell proliferation cellular distribution controlled study cytoplasm female in vitro study in vivo study intracellular space mouse nonhuman priority journal protein analysis protein localization protein modification squamous cell carcinoma active transport animal drug effect fluorescent antibody technique metabolism nude mouse sensitive to carcinogenesis mouse tumor cell line Western blotting Active Transport, Cell Nucleus Animals Blotting, Western Catalase Cell Line, Tumor Cell Nucleus Cyclin D1 Cyclin-Dependent Kinase Inhibitor p27 Female Fluorescent Antibody Technique Free Radical Scavengers G1 Phase Hydrogen Peroxide Mice Mice, Inbred SENCAR Mice, Nude Reactive Oxygen Species S Phase |
spellingShingle |
Cancer cells Cell cycle Cyclin D1 Hydrogen peroxide P27kip1 Proliferation catalase cyclin D1 cyclin dependent kinase inhibitor 1B hydrogen peroxide catalase cyclin D1 cyclin dependent kinase inhibitor 1B hydrogen peroxide reactive oxygen metabolite scavenger animal cell animal experiment animal model animal tissue antineoplastic activity article cancer inhibition carcinoma cell cell cycle arrest cell cycle G1 phase cell cycle regulation cell cycle S phase cell level cell nucleus cell proliferation cellular distribution controlled study cytoplasm female in vitro study in vivo study intracellular space mouse nonhuman priority journal protein analysis protein localization protein modification squamous cell carcinoma active transport animal drug effect fluorescent antibody technique metabolism nude mouse sensitive to carcinogenesis mouse tumor cell line Western blotting Active Transport, Cell Nucleus Animals Blotting, Western Catalase Cell Line, Tumor Cell Nucleus Cyclin D1 Cyclin-Dependent Kinase Inhibitor p27 Female Fluorescent Antibody Technique Free Radical Scavengers G1 Phase Hydrogen Peroxide Mice Mice, Inbred SENCAR Mice, Nude Reactive Oxygen Species S Phase Policastro, Lucía Laura Palmieri, Mónica Alejandra Molinari de Rey, Beatriz Durán, Hebe Alicia H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1 |
topic_facet |
Cancer cells Cell cycle Cyclin D1 Hydrogen peroxide P27kip1 Proliferation catalase cyclin D1 cyclin dependent kinase inhibitor 1B hydrogen peroxide catalase cyclin D1 cyclin dependent kinase inhibitor 1B hydrogen peroxide reactive oxygen metabolite scavenger animal cell animal experiment animal model animal tissue antineoplastic activity article cancer inhibition carcinoma cell cell cycle arrest cell cycle G1 phase cell cycle regulation cell cycle S phase cell level cell nucleus cell proliferation cellular distribution controlled study cytoplasm female in vitro study in vivo study intracellular space mouse nonhuman priority journal protein analysis protein localization protein modification squamous cell carcinoma active transport animal drug effect fluorescent antibody technique metabolism nude mouse sensitive to carcinogenesis mouse tumor cell line Western blotting Active Transport, Cell Nucleus Animals Blotting, Western Catalase Cell Line, Tumor Cell Nucleus Cyclin D1 Cyclin-Dependent Kinase Inhibitor p27 Female Fluorescent Antibody Technique Free Radical Scavengers G1 Phase Hydrogen Peroxide Mice Mice, Inbred SENCAR Mice, Nude Reactive Oxygen Species S Phase |
description |
The aim of the present study was to evaluate cell cycle regulation by scavenging H2O2 in tumor cells. A significant arrest in the G1 phase of the cell cycle was demonstrated in CH72-T4 carcinoma cells exposed to catalase, associated with a decrease in cyclin D1 and an increase in the CDK inhibitory protein p27KIP1. Moreover, we found a differential intracellular distribution of p27KIP1, which remained in the nucleus after catalase treatment. In vivo experiments showed an increase in nuclear levels of p27KIP1 associated with the inhibition of tumor growth by H2O2 scavenging, confirming in vitro results. To conclude, H2O2 scavenging may induce cell cycle arrest through the modulation of cyclin D1 and p27KIP1 levels and nuclear localization of p27KIP1. To our knowledge, this is the first report that demonstrates that the modulation of ROS alters the intracellular localization of a key regulatory protein of G1/S transition. © 2011 Elsevier Ireland Ltd. |
author |
Policastro, Lucía Laura Palmieri, Mónica Alejandra Molinari de Rey, Beatriz Durán, Hebe Alicia |
author_facet |
Policastro, Lucía Laura Palmieri, Mónica Alejandra Molinari de Rey, Beatriz Durán, Hebe Alicia |
author_sort |
Policastro, Lucía Laura |
title |
H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1 |
title_short |
H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1 |
title_full |
H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1 |
title_fullStr |
H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1 |
title_full_unstemmed |
H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1 |
title_sort |
h2o2 scavenging inhibits g1/s transition by increasing nuclear levels of p27kip1 |
publishDate |
2011 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043835_v305_n1_p58_Ibanez http://hdl.handle.net/20.500.12110/paper_03043835_v305_n1_p58_Ibanez |
work_keys_str_mv |
AT policastrolucialaura h2o2scavenginginhibitsg1stransitionbyincreasingnuclearlevelsofp27kip1 AT palmierimonicaalejandra h2o2scavenginginhibitsg1stransitionbyincreasingnuclearlevelsofp27kip1 AT molinaridereybeatriz h2o2scavenginginhibitsg1stransitionbyincreasingnuclearlevelsofp27kip1 AT duranhebealicia h2o2scavenginginhibitsg1stransitionbyincreasingnuclearlevelsofp27kip1 |
_version_ |
1768544453214076928 |