Extracellular matrix components regulate ACTH production and proliferation in corticotroph tumor cells
The extracellular matrix (ECM) conveys signals through membrane receptors called integrins producing changes in cell morphology, proliferation, differentiation and apoptosis. Previous studies suggest that the ECM plays an important role in pituitary physiology and tumorigenesis. In the present work...
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2001
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v175_n1-2_p141_Kuchenbauer http://hdl.handle.net/20.500.12110/paper_03037207_v175_n1-2_p141_Kuchenbauer |
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paper:paper_03037207_v175_n1-2_p141_Kuchenbauer2023-06-08T15:29:01Z Extracellular matrix components regulate ACTH production and proliferation in corticotroph tumor cells Páez Pereda, Marcelo ACTH Corticotroph cells Extracellular matrix Integrins POMC Reactive oxygen species collagen type 1 collagen type 4 corticotropin fibronectin integrin laminin reactive oxygen metabolite article cell proliferation cell structure controlled study corticotropin release extracellular matrix genetic transcription hormone release human human cell hypophysis cell priority journal signal transduction tumor cell Adrenocorticotropic Hormone Animals Cell Division Collagen Extracellular Matrix Fibronectins Laminin Mice Pituitary Neoplasms Pro-Opiomelanocortin Promoter Regions (Genetics) Transcription, Genetic Tumor Cells, Cultured The extracellular matrix (ECM) conveys signals through membrane receptors called integrins producing changes in cell morphology, proliferation, differentiation and apoptosis. Previous studies suggest that the ECM plays an important role in pituitary physiology and tumorigenesis. In the present work we studied for the first time the effects of fibronectin, laminin, collagen I and collagen IV on hormone secretion and cell proliferation in the corticotroph tumor cell line AtT-20 and in normal pituitary cells, examining the signal transduction mechanisms that mediate these effects. ACTH production in AtT-20 cells was inhibited by fibronectin, laminin and collagen I. A reporter construct with the POMC promoter showed similar results, indicating that the effects of the ECM take place at the level of POMC gene transcription. In contrast, ACTH production was not significantly altered in normal pituitary cells. AtT-20 cell proliferation was stimulated by collagen IV and fibronectin, but inhibited by collagen I and laminin. In parallel, the cell morphology was modified by the ECM. We found that the production of reactive oxygen species mediate the effects of laminin and collagen IV. On the other hand, the effect of fibronectin was mimicked by β1-integrin and Rho activation. These results show for the first time that the ECM controls ACTH biosynthesis and proliferation in corticotroph tumor cells and suggest a role for the ECM in corticotroph adenoma development. Copyright © 2001 Elsevier Science Ireland Ltd. Fil:Páez-Pereda, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v175_n1-2_p141_Kuchenbauer http://hdl.handle.net/20.500.12110/paper_03037207_v175_n1-2_p141_Kuchenbauer |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
ACTH Corticotroph cells Extracellular matrix Integrins POMC Reactive oxygen species collagen type 1 collagen type 4 corticotropin fibronectin integrin laminin reactive oxygen metabolite article cell proliferation cell structure controlled study corticotropin release extracellular matrix genetic transcription hormone release human human cell hypophysis cell priority journal signal transduction tumor cell Adrenocorticotropic Hormone Animals Cell Division Collagen Extracellular Matrix Fibronectins Laminin Mice Pituitary Neoplasms Pro-Opiomelanocortin Promoter Regions (Genetics) Transcription, Genetic Tumor Cells, Cultured |
| spellingShingle |
ACTH Corticotroph cells Extracellular matrix Integrins POMC Reactive oxygen species collagen type 1 collagen type 4 corticotropin fibronectin integrin laminin reactive oxygen metabolite article cell proliferation cell structure controlled study corticotropin release extracellular matrix genetic transcription hormone release human human cell hypophysis cell priority journal signal transduction tumor cell Adrenocorticotropic Hormone Animals Cell Division Collagen Extracellular Matrix Fibronectins Laminin Mice Pituitary Neoplasms Pro-Opiomelanocortin Promoter Regions (Genetics) Transcription, Genetic Tumor Cells, Cultured Páez Pereda, Marcelo Extracellular matrix components regulate ACTH production and proliferation in corticotroph tumor cells |
| topic_facet |
ACTH Corticotroph cells Extracellular matrix Integrins POMC Reactive oxygen species collagen type 1 collagen type 4 corticotropin fibronectin integrin laminin reactive oxygen metabolite article cell proliferation cell structure controlled study corticotropin release extracellular matrix genetic transcription hormone release human human cell hypophysis cell priority journal signal transduction tumor cell Adrenocorticotropic Hormone Animals Cell Division Collagen Extracellular Matrix Fibronectins Laminin Mice Pituitary Neoplasms Pro-Opiomelanocortin Promoter Regions (Genetics) Transcription, Genetic Tumor Cells, Cultured |
| description |
The extracellular matrix (ECM) conveys signals through membrane receptors called integrins producing changes in cell morphology, proliferation, differentiation and apoptosis. Previous studies suggest that the ECM plays an important role in pituitary physiology and tumorigenesis. In the present work we studied for the first time the effects of fibronectin, laminin, collagen I and collagen IV on hormone secretion and cell proliferation in the corticotroph tumor cell line AtT-20 and in normal pituitary cells, examining the signal transduction mechanisms that mediate these effects. ACTH production in AtT-20 cells was inhibited by fibronectin, laminin and collagen I. A reporter construct with the POMC promoter showed similar results, indicating that the effects of the ECM take place at the level of POMC gene transcription. In contrast, ACTH production was not significantly altered in normal pituitary cells. AtT-20 cell proliferation was stimulated by collagen IV and fibronectin, but inhibited by collagen I and laminin. In parallel, the cell morphology was modified by the ECM. We found that the production of reactive oxygen species mediate the effects of laminin and collagen IV. On the other hand, the effect of fibronectin was mimicked by β1-integrin and Rho activation. These results show for the first time that the ECM controls ACTH biosynthesis and proliferation in corticotroph tumor cells and suggest a role for the ECM in corticotroph adenoma development. Copyright © 2001 Elsevier Science Ireland Ltd. |
| author |
Páez Pereda, Marcelo |
| author_facet |
Páez Pereda, Marcelo |
| author_sort |
Páez Pereda, Marcelo |
| title |
Extracellular matrix components regulate ACTH production and proliferation in corticotroph tumor cells |
| title_short |
Extracellular matrix components regulate ACTH production and proliferation in corticotroph tumor cells |
| title_full |
Extracellular matrix components regulate ACTH production and proliferation in corticotroph tumor cells |
| title_fullStr |
Extracellular matrix components regulate ACTH production and proliferation in corticotroph tumor cells |
| title_full_unstemmed |
Extracellular matrix components regulate ACTH production and proliferation in corticotroph tumor cells |
| title_sort |
extracellular matrix components regulate acth production and proliferation in corticotroph tumor cells |
| publishDate |
2001 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v175_n1-2_p141_Kuchenbauer http://hdl.handle.net/20.500.12110/paper_03037207_v175_n1-2_p141_Kuchenbauer |
| work_keys_str_mv |
AT paezperedamarcelo extracellularmatrixcomponentsregulateacthproductionandproliferationincorticotrophtumorcells |
| _version_ |
1768546067765264384 |