Interleukin-2 (IL-2) and IL-6 regulate c-fos protooncogene expression in human pituitary adenoma explants
We have previously shown that interleukin-2 (IL-2) and IL-6, which are expressed in the anterior pituitary, affect anterior pituitary cell proliferation in normal rats and cell lines. Here we examined their effects on the c-fos expression by human anterior pituitary adenomas. Adenoma cells in cultur...
Guardado en:
| Autor principal: | |
|---|---|
| Publicado: |
1996
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v124_n1-2_p33_PaezPereda http://hdl.handle.net/20.500.12110/paper_03037207_v124_n1-2_p33_PaezPereda |
| Aporte de: |
| id |
paper:paper_03037207_v124_n1-2_p33_PaezPereda |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_03037207_v124_n1-2_p33_PaezPereda2023-06-08T15:28:59Z Interleukin-2 (IL-2) and IL-6 regulate c-fos protooncogene expression in human pituitary adenoma explants Páez Pereda, Marcelo c-fos Interleukin-2 Interleukin-6 Pituitary adenomas blocking antibody corticotropin growth hormone interleukin 2 interleukin 2 receptor antibody interleukin 6 interleukin 6 antibody messenger rna prolactin adenohypophysis adult article cell proliferation controlled study corticotropin release explant female gene expression regulation growth hormone release human human cell hypophysis adenoma immunohistochemistry male northern blotting oncogene c fos priority journal prolactin release proto oncogene Adenoma Adolescent Adult Female Gene Expression Regulation, Neoplastic Genes, fos Humans Interleukin-2 Interleukin-6 Male Middle Aged Pituitary Gland, Anterior Pituitary Neoplasms RNA, Messenger RNA, Neoplasm Tumor Cells, Cultured We have previously shown that interleukin-2 (IL-2) and IL-6, which are expressed in the anterior pituitary, affect anterior pituitary cell proliferation in normal rats and cell lines. Here we examined their effects on the c-fos expression by human anterior pituitary adenomas. Adenoma cells in culture do not express c-fos mRNA. In adenoma explants, however, c-fos expression was detected and was regulated by IL-2 or IL-6. In different tumors (ACTH-, PRL-, GH-secreting and non functioning adenomas), these interleukins had inhibitory or stimulatory effects but the kind of response does not seem to be associated to tumor type or size. Using blocking antibodies, we observed that intrinsic IL-2 and IL-6 regulate c-fos expression in the same way. Our data suggest that IL-2 and IL-6 are not only involved in the regulation of pituitary adenoma function but may also, given the role of c-fos in cell proliferation, be implicated in the development of human pituitary adenomas. Fil:Páez Pereda, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v124_n1-2_p33_PaezPereda http://hdl.handle.net/20.500.12110/paper_03037207_v124_n1-2_p33_PaezPereda |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
c-fos Interleukin-2 Interleukin-6 Pituitary adenomas blocking antibody corticotropin growth hormone interleukin 2 interleukin 2 receptor antibody interleukin 6 interleukin 6 antibody messenger rna prolactin adenohypophysis adult article cell proliferation controlled study corticotropin release explant female gene expression regulation growth hormone release human human cell hypophysis adenoma immunohistochemistry male northern blotting oncogene c fos priority journal prolactin release proto oncogene Adenoma Adolescent Adult Female Gene Expression Regulation, Neoplastic Genes, fos Humans Interleukin-2 Interleukin-6 Male Middle Aged Pituitary Gland, Anterior Pituitary Neoplasms RNA, Messenger RNA, Neoplasm Tumor Cells, Cultured |
| spellingShingle |
c-fos Interleukin-2 Interleukin-6 Pituitary adenomas blocking antibody corticotropin growth hormone interleukin 2 interleukin 2 receptor antibody interleukin 6 interleukin 6 antibody messenger rna prolactin adenohypophysis adult article cell proliferation controlled study corticotropin release explant female gene expression regulation growth hormone release human human cell hypophysis adenoma immunohistochemistry male northern blotting oncogene c fos priority journal prolactin release proto oncogene Adenoma Adolescent Adult Female Gene Expression Regulation, Neoplastic Genes, fos Humans Interleukin-2 Interleukin-6 Male Middle Aged Pituitary Gland, Anterior Pituitary Neoplasms RNA, Messenger RNA, Neoplasm Tumor Cells, Cultured Páez Pereda, Marcelo Interleukin-2 (IL-2) and IL-6 regulate c-fos protooncogene expression in human pituitary adenoma explants |
| topic_facet |
c-fos Interleukin-2 Interleukin-6 Pituitary adenomas blocking antibody corticotropin growth hormone interleukin 2 interleukin 2 receptor antibody interleukin 6 interleukin 6 antibody messenger rna prolactin adenohypophysis adult article cell proliferation controlled study corticotropin release explant female gene expression regulation growth hormone release human human cell hypophysis adenoma immunohistochemistry male northern blotting oncogene c fos priority journal prolactin release proto oncogene Adenoma Adolescent Adult Female Gene Expression Regulation, Neoplastic Genes, fos Humans Interleukin-2 Interleukin-6 Male Middle Aged Pituitary Gland, Anterior Pituitary Neoplasms RNA, Messenger RNA, Neoplasm Tumor Cells, Cultured |
| description |
We have previously shown that interleukin-2 (IL-2) and IL-6, which are expressed in the anterior pituitary, affect anterior pituitary cell proliferation in normal rats and cell lines. Here we examined their effects on the c-fos expression by human anterior pituitary adenomas. Adenoma cells in culture do not express c-fos mRNA. In adenoma explants, however, c-fos expression was detected and was regulated by IL-2 or IL-6. In different tumors (ACTH-, PRL-, GH-secreting and non functioning adenomas), these interleukins had inhibitory or stimulatory effects but the kind of response does not seem to be associated to tumor type or size. Using blocking antibodies, we observed that intrinsic IL-2 and IL-6 regulate c-fos expression in the same way. Our data suggest that IL-2 and IL-6 are not only involved in the regulation of pituitary adenoma function but may also, given the role of c-fos in cell proliferation, be implicated in the development of human pituitary adenomas. |
| author |
Páez Pereda, Marcelo |
| author_facet |
Páez Pereda, Marcelo |
| author_sort |
Páez Pereda, Marcelo |
| title |
Interleukin-2 (IL-2) and IL-6 regulate c-fos protooncogene expression in human pituitary adenoma explants |
| title_short |
Interleukin-2 (IL-2) and IL-6 regulate c-fos protooncogene expression in human pituitary adenoma explants |
| title_full |
Interleukin-2 (IL-2) and IL-6 regulate c-fos protooncogene expression in human pituitary adenoma explants |
| title_fullStr |
Interleukin-2 (IL-2) and IL-6 regulate c-fos protooncogene expression in human pituitary adenoma explants |
| title_full_unstemmed |
Interleukin-2 (IL-2) and IL-6 regulate c-fos protooncogene expression in human pituitary adenoma explants |
| title_sort |
interleukin-2 (il-2) and il-6 regulate c-fos protooncogene expression in human pituitary adenoma explants |
| publishDate |
1996 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v124_n1-2_p33_PaezPereda http://hdl.handle.net/20.500.12110/paper_03037207_v124_n1-2_p33_PaezPereda |
| work_keys_str_mv |
AT paezperedamarcelo interleukin2il2andil6regulatecfosprotooncogeneexpressioninhumanpituitaryadenomaexplants |
| _version_ |
1768546067577569280 |