Characterization of the ETSA-21 antigen, a glycosylphosphatidyl-inositol anchor glycoprotein identified in breast cancer cells using monoclonal antibody B21
Mab B21 is a monoclonal antibody (Mab) that recognizes an epithelial tumor surface antigen (ETSA-B21) from diverse human tumor cell lines including breast, ovary, uterus, and their cognate carcinoma tissues. A lower reactivity has been observed in normal breast tissue and benign hyperplesia. In this...
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1997
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0272457X_v16_n2_p139_Annibali http://hdl.handle.net/20.500.12110/paper_0272457X_v16_n2_p139_Annibali |
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paper:paper_0272457X_v16_n2_p139_Annibali2023-06-08T15:25:07Z Characterization of the ETSA-21 antigen, a glycosylphosphatidyl-inositol anchor glycoprotein identified in breast cancer cells using monoclonal antibody B21 glycoprotein glycosylphosphatidylinositol membrane antigen monoclonal antibody phosphatidylinositol phospholipase C sialidase tumor antigen tunicamycin antigen antibody complex antigen detection antigen expression article breast cancer breast hyperplasia cancer cell culture controlled study human human cell immunofluorescence microscopy internalization priority journal protein glycosylation signal transduction Mink cell focus-forming virus Mab B21 is a monoclonal antibody (Mab) that recognizes an epithelial tumor surface antigen (ETSA-B21) from diverse human tumor cell lines including breast, ovary, uterus, and their cognate carcinoma tissues. A lower reactivity has been observed in normal breast tissue and benign hyperplesia. In this study, the characteristics of the ETSA-B21 antigen have been examined in greater detail in the MCF-7, SK-BR-3, and MDA-MB-453 breast cancer cell lines. Treatment with phosphatidylinositol-phospholipase C, but no neuraminidase were found to partially remove the ETSA-B21 signal from the cell surface as revealed by immunofluorescence microscopy. Inhibition of the N-glycosylation pathway by tunicamycin resulted in a decreased ETSA-B21 signal on the cell membrane. In addition, the antigen-antibody complex was internalized in breast cancer cells as demonstrated by an acidic wash internalization assay evaluated using immunofluorescence. In conclusion, this study suggests that ETSA-B21 is a GPI anchor N-glycosylated protein promoting specific antibody internalization in breast cancer cells. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0272457X_v16_n2_p139_Annibali http://hdl.handle.net/20.500.12110/paper_0272457X_v16_n2_p139_Annibali |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
glycoprotein glycosylphosphatidylinositol membrane antigen monoclonal antibody phosphatidylinositol phospholipase C sialidase tumor antigen tunicamycin antigen antibody complex antigen detection antigen expression article breast cancer breast hyperplasia cancer cell culture controlled study human human cell immunofluorescence microscopy internalization priority journal protein glycosylation signal transduction Mink cell focus-forming virus |
| spellingShingle |
glycoprotein glycosylphosphatidylinositol membrane antigen monoclonal antibody phosphatidylinositol phospholipase C sialidase tumor antigen tunicamycin antigen antibody complex antigen detection antigen expression article breast cancer breast hyperplasia cancer cell culture controlled study human human cell immunofluorescence microscopy internalization priority journal protein glycosylation signal transduction Mink cell focus-forming virus Characterization of the ETSA-21 antigen, a glycosylphosphatidyl-inositol anchor glycoprotein identified in breast cancer cells using monoclonal antibody B21 |
| topic_facet |
glycoprotein glycosylphosphatidylinositol membrane antigen monoclonal antibody phosphatidylinositol phospholipase C sialidase tumor antigen tunicamycin antigen antibody complex antigen detection antigen expression article breast cancer breast hyperplasia cancer cell culture controlled study human human cell immunofluorescence microscopy internalization priority journal protein glycosylation signal transduction Mink cell focus-forming virus |
| description |
Mab B21 is a monoclonal antibody (Mab) that recognizes an epithelial tumor surface antigen (ETSA-B21) from diverse human tumor cell lines including breast, ovary, uterus, and their cognate carcinoma tissues. A lower reactivity has been observed in normal breast tissue and benign hyperplesia. In this study, the characteristics of the ETSA-B21 antigen have been examined in greater detail in the MCF-7, SK-BR-3, and MDA-MB-453 breast cancer cell lines. Treatment with phosphatidylinositol-phospholipase C, but no neuraminidase were found to partially remove the ETSA-B21 signal from the cell surface as revealed by immunofluorescence microscopy. Inhibition of the N-glycosylation pathway by tunicamycin resulted in a decreased ETSA-B21 signal on the cell membrane. In addition, the antigen-antibody complex was internalized in breast cancer cells as demonstrated by an acidic wash internalization assay evaluated using immunofluorescence. In conclusion, this study suggests that ETSA-B21 is a GPI anchor N-glycosylated protein promoting specific antibody internalization in breast cancer cells. |
| title |
Characterization of the ETSA-21 antigen, a glycosylphosphatidyl-inositol anchor glycoprotein identified in breast cancer cells using monoclonal antibody B21 |
| title_short |
Characterization of the ETSA-21 antigen, a glycosylphosphatidyl-inositol anchor glycoprotein identified in breast cancer cells using monoclonal antibody B21 |
| title_full |
Characterization of the ETSA-21 antigen, a glycosylphosphatidyl-inositol anchor glycoprotein identified in breast cancer cells using monoclonal antibody B21 |
| title_fullStr |
Characterization of the ETSA-21 antigen, a glycosylphosphatidyl-inositol anchor glycoprotein identified in breast cancer cells using monoclonal antibody B21 |
| title_full_unstemmed |
Characterization of the ETSA-21 antigen, a glycosylphosphatidyl-inositol anchor glycoprotein identified in breast cancer cells using monoclonal antibody B21 |
| title_sort |
characterization of the etsa-21 antigen, a glycosylphosphatidyl-inositol anchor glycoprotein identified in breast cancer cells using monoclonal antibody b21 |
| publishDate |
1997 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0272457X_v16_n2_p139_Annibali http://hdl.handle.net/20.500.12110/paper_0272457X_v16_n2_p139_Annibali |
| _version_ |
1768541892229726208 |