A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus
Neurogenesis in the dentate gyrus continues into adulthood, yet little is known about the function of newly born neurons or how they integrate into an existing network of mature neurons. We made transgenic mice that selectively and transiently express enhanced green fluorescent protein (EGFP) in new...
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2004
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02706474_v24_n13_p3251_Overstreet http://hdl.handle.net/20.500.12110/paper_02706474_v24_n13_p3251_Overstreet |
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paper:paper_02706474_v24_n13_p3251_Overstreet2023-06-08T15:24:44Z A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus BrdU Dentate granule cell Green fluorescent protein Neurogenesis Neuronal progenitor Proopiomelanocortin PSA-nCAM Transgenic mice broxuridine green fluorescent protein proopiomelanocortin action potential animal tissue article dentate gyrus gene gene expression granule cell immunohistochemistry mouse newborn nonhuman priority journal stem cell Action Potentials Aging Animals Biological Markers Bromodeoxyuridine Cell Count Cell Division Cell Movement Dentate Gyrus Exertion Genes, Reporter Green Fluorescent Proteins Luminescent Proteins Mice Mice, Inbred C57BL Mice, Transgenic Neural Cell Adhesion Molecule L1 Neurons Pro-Opiomelanocortin Promoter Regions (Genetics) Sialic Acids Transgenes Neurogenesis in the dentate gyrus continues into adulthood, yet little is known about the function of newly born neurons or how they integrate into an existing network of mature neurons. We made transgenic mice that selectively and transiently express enhanced green fluorescent protein (EGFP) in newly born granule cells of the dentate gyrus under the transcriptional control of proopiomelanocortin (POMC) genomic sequences. Analysis of transgenic pedigrees with truncation or deletion mutations indicated that EGFP expression in the dentate gyrus required cryptic POMC promoter regions dispensable for arcuate hypothalamic or pituitary expression. Unlike arcuate neurons, dentate granule cells did not express the endogenous POMC gene. EGFP-positive neurons had immature properties, including short spineless dendrites and small action potentials. Colocalization with bromodeoxyuridine indicated that EGFP-labeled granule cells were ∼2 weeks postmitotic. EGFP-labeled cells expressed markers for immature granule cells but not the glial marker GFAP. The number of EGFP-labeled neurons declined with age and increased with exercise, paralleling neurogenesis. Our results indicate that POMC-EGFP marks immature granule cells and that adult-generated granule cells integrate quite slowly into the hippocampal circuitry. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02706474_v24_n13_p3251_Overstreet http://hdl.handle.net/20.500.12110/paper_02706474_v24_n13_p3251_Overstreet |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
BrdU Dentate granule cell Green fluorescent protein Neurogenesis Neuronal progenitor Proopiomelanocortin PSA-nCAM Transgenic mice broxuridine green fluorescent protein proopiomelanocortin action potential animal tissue article dentate gyrus gene gene expression granule cell immunohistochemistry mouse newborn nonhuman priority journal stem cell Action Potentials Aging Animals Biological Markers Bromodeoxyuridine Cell Count Cell Division Cell Movement Dentate Gyrus Exertion Genes, Reporter Green Fluorescent Proteins Luminescent Proteins Mice Mice, Inbred C57BL Mice, Transgenic Neural Cell Adhesion Molecule L1 Neurons Pro-Opiomelanocortin Promoter Regions (Genetics) Sialic Acids Transgenes |
spellingShingle |
BrdU Dentate granule cell Green fluorescent protein Neurogenesis Neuronal progenitor Proopiomelanocortin PSA-nCAM Transgenic mice broxuridine green fluorescent protein proopiomelanocortin action potential animal tissue article dentate gyrus gene gene expression granule cell immunohistochemistry mouse newborn nonhuman priority journal stem cell Action Potentials Aging Animals Biological Markers Bromodeoxyuridine Cell Count Cell Division Cell Movement Dentate Gyrus Exertion Genes, Reporter Green Fluorescent Proteins Luminescent Proteins Mice Mice, Inbred C57BL Mice, Transgenic Neural Cell Adhesion Molecule L1 Neurons Pro-Opiomelanocortin Promoter Regions (Genetics) Sialic Acids Transgenes A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus |
topic_facet |
BrdU Dentate granule cell Green fluorescent protein Neurogenesis Neuronal progenitor Proopiomelanocortin PSA-nCAM Transgenic mice broxuridine green fluorescent protein proopiomelanocortin action potential animal tissue article dentate gyrus gene gene expression granule cell immunohistochemistry mouse newborn nonhuman priority journal stem cell Action Potentials Aging Animals Biological Markers Bromodeoxyuridine Cell Count Cell Division Cell Movement Dentate Gyrus Exertion Genes, Reporter Green Fluorescent Proteins Luminescent Proteins Mice Mice, Inbred C57BL Mice, Transgenic Neural Cell Adhesion Molecule L1 Neurons Pro-Opiomelanocortin Promoter Regions (Genetics) Sialic Acids Transgenes |
description |
Neurogenesis in the dentate gyrus continues into adulthood, yet little is known about the function of newly born neurons or how they integrate into an existing network of mature neurons. We made transgenic mice that selectively and transiently express enhanced green fluorescent protein (EGFP) in newly born granule cells of the dentate gyrus under the transcriptional control of proopiomelanocortin (POMC) genomic sequences. Analysis of transgenic pedigrees with truncation or deletion mutations indicated that EGFP expression in the dentate gyrus required cryptic POMC promoter regions dispensable for arcuate hypothalamic or pituitary expression. Unlike arcuate neurons, dentate granule cells did not express the endogenous POMC gene. EGFP-positive neurons had immature properties, including short spineless dendrites and small action potentials. Colocalization with bromodeoxyuridine indicated that EGFP-labeled granule cells were ∼2 weeks postmitotic. EGFP-labeled cells expressed markers for immature granule cells but not the glial marker GFAP. The number of EGFP-labeled neurons declined with age and increased with exercise, paralleling neurogenesis. Our results indicate that POMC-EGFP marks immature granule cells and that adult-generated granule cells integrate quite slowly into the hippocampal circuitry. |
title |
A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus |
title_short |
A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus |
title_full |
A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus |
title_fullStr |
A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus |
title_full_unstemmed |
A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus |
title_sort |
transgenic marker for newly born granule cells in dentate gyrus |
publishDate |
2004 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02706474_v24_n13_p3251_Overstreet http://hdl.handle.net/20.500.12110/paper_02706474_v24_n13_p3251_Overstreet |
_version_ |
1768543275706220544 |