Cell-type specific regulation of galectin-3 expression by glucocorticoids in lung Clara cells and macrophages

Bronchiolar Clara cells are integral components of lung homeostasis, predominantly distributed in distal airways. In addition to the 16 kDa Clara cell protein, a major secretory product with antiinflammatory effects, rat Clara cells express the glycanbinding protein galectin-3 and secrete it into th...

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Autores principales:	Sundblad, Victoria, Salatino, Mariana, Croci Russo, Diego Omar
Publicado:	2011
Materias:	Bronchiolar clara cells Galectin-3 Glucocorticoids Macrophages Animalia Rattus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02133911_v26_n6_p747_Maldonado http://hdl.handle.net/20.500.12110/paper_02133911_v26_n6_p747_Maldonado
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_02133911_v26_n6_p747_Maldonado
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spelling	paper:paper_02133911_v26_n6_p747_Maldonado2023-06-08T15:20:48Z Cell-type specific regulation of galectin-3 expression by glucocorticoids in lung Clara cells and macrophages Sundblad, Victoria Salatino, Mariana Croci Russo, Diego Omar Bronchiolar clara cells Galectin-3 Glucocorticoids Macrophages Animalia Rattus Bronchiolar Clara cells are integral components of lung homeostasis, predominantly distributed in distal airways. In addition to the 16 kDa Clara cell protein, a major secretory product with antiinflammatory effects, rat Clara cells express the glycanbinding protein galectin-3 and secrete it into the airways. Given the essential role of galectin-3 in the control of inflammation and the well-established function of glucocorticoids (GCs) in lung physiology, here we investigated whether galectin-3 is a target of the regulatory effects of GCs. Adult male rats were subjected to bilateral adrenalectomy and the lungs were processed for light and transmission electron microscopy, immunoelectron microscopy and Western blot analysis. Profound changes in bronchiolar Clara cells and macrophage morphology could be observed by electron microscopy after adrenalectomy. While specific galectin-3 staining was detected in the nucleus and cytoplasm of Clara cells and macrophages from control animals, cytoplasmic galectin-3 expression was dramatically reduced after adrenalectomy in both cell types. This effect was cell-specific as it did not affect expression of this lectin in ciliated cells. After dexamethasone treatment, galectin-3 expression increased significantly in the nucleus and cytoplasm of macrophages and Clara cells. Western blot analysis showed a clear decrease in galectin-3 expression in ADX animals, which was recovered after a 7-day treatment with dexamethasone. In peritoneal macrophages, galectin-3 expression was also dependent on the effects of GCs both in vivo and in vitro. Our results identify a cell type-specific control of galectin-3 synthesis by GCs in lung bronchiolar Clara cells and interstitial macrophages, which may provide an alternative mechanism by which GCs contribute to modulate the inflammatory response. Fil:Sundblad, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Salatino, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Croci, D.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02133911_v26_n6_p747_Maldonado http://hdl.handle.net/20.500.12110/paper_02133911_v26_n6_p747_Maldonado
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bronchiolar clara cells Galectin-3 Glucocorticoids Macrophages Animalia Rattus
spellingShingle	Bronchiolar clara cells Galectin-3 Glucocorticoids Macrophages Animalia Rattus Sundblad, Victoria Salatino, Mariana Croci Russo, Diego Omar Cell-type specific regulation of galectin-3 expression by glucocorticoids in lung Clara cells and macrophages
topic_facet	Bronchiolar clara cells Galectin-3 Glucocorticoids Macrophages Animalia Rattus
description	Bronchiolar Clara cells are integral components of lung homeostasis, predominantly distributed in distal airways. In addition to the 16 kDa Clara cell protein, a major secretory product with antiinflammatory effects, rat Clara cells express the glycanbinding protein galectin-3 and secrete it into the airways. Given the essential role of galectin-3 in the control of inflammation and the well-established function of glucocorticoids (GCs) in lung physiology, here we investigated whether galectin-3 is a target of the regulatory effects of GCs. Adult male rats were subjected to bilateral adrenalectomy and the lungs were processed for light and transmission electron microscopy, immunoelectron microscopy and Western blot analysis. Profound changes in bronchiolar Clara cells and macrophage morphology could be observed by electron microscopy after adrenalectomy. While specific galectin-3 staining was detected in the nucleus and cytoplasm of Clara cells and macrophages from control animals, cytoplasmic galectin-3 expression was dramatically reduced after adrenalectomy in both cell types. This effect was cell-specific as it did not affect expression of this lectin in ciliated cells. After dexamethasone treatment, galectin-3 expression increased significantly in the nucleus and cytoplasm of macrophages and Clara cells. Western blot analysis showed a clear decrease in galectin-3 expression in ADX animals, which was recovered after a 7-day treatment with dexamethasone. In peritoneal macrophages, galectin-3 expression was also dependent on the effects of GCs both in vivo and in vitro. Our results identify a cell type-specific control of galectin-3 synthesis by GCs in lung bronchiolar Clara cells and interstitial macrophages, which may provide an alternative mechanism by which GCs contribute to modulate the inflammatory response.
author	Sundblad, Victoria Salatino, Mariana Croci Russo, Diego Omar
author_facet	Sundblad, Victoria Salatino, Mariana Croci Russo, Diego Omar
author_sort	Sundblad, Victoria
title	Cell-type specific regulation of galectin-3 expression by glucocorticoids in lung Clara cells and macrophages
title_short	Cell-type specific regulation of galectin-3 expression by glucocorticoids in lung Clara cells and macrophages
title_full	Cell-type specific regulation of galectin-3 expression by glucocorticoids in lung Clara cells and macrophages
title_fullStr	Cell-type specific regulation of galectin-3 expression by glucocorticoids in lung Clara cells and macrophages
title_full_unstemmed	Cell-type specific regulation of galectin-3 expression by glucocorticoids in lung Clara cells and macrophages
title_sort	cell-type specific regulation of galectin-3 expression by glucocorticoids in lung clara cells and macrophages
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02133911_v26_n6_p747_Maldonado http://hdl.handle.net/20.500.12110/paper_02133911_v26_n6_p747_Maldonado
work_keys_str_mv	AT sundbladvictoria celltypespecificregulationofgalectin3expressionbyglucocorticoidsinlungclaracellsandmacrophages AT salatinomariana celltypespecificregulationofgalectin3expressionbyglucocorticoidsinlungclaracellsandmacrophages AT crocirussodiegoomar celltypespecificregulationofgalectin3expressionbyglucocorticoidsinlungclaracellsandmacrophages
_version_	1768541889151107072

Cell-type specific regulation of galectin-3 expression by glucocorticoids in lung Clara cells and macrophages

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