Production of compact petunias through polyploidization

Petunia, a commercially important ornamental plant worldwide, has been subjected to breeding programs that have yielded a high number of varieties. One of the key factors in the commercial value of these varieties is plant compactness. Currently, compact petunias are obtained through the application...

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Autor principal:	Vélez, Carlos Guillermo
Publicado:	2017
Materias:	Autotetraploids Colchicine Organogenesis Ornamentals Petunia axillaris Plant architecture Plant compactness, dwarfing Chromosomes Plants (botany) Breeding Plants
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676857_v129_n1_p61_Regalado http://hdl.handle.net/20.500.12110/paper_01676857_v129_n1_p61_Regalado
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01676857_v129_n1_p61_Regalado
record_format	dspace
spelling	paper:paper_01676857_v129_n1_p61_Regalado2023-06-08T15:16:44Z Production of compact petunias through polyploidization Vélez, Carlos Guillermo Autotetraploids Colchicine Organogenesis Ornamentals Petunia axillaris Plant architecture Plant compactness, dwarfing Chromosomes Autotetraploids Colchicine Organogenesis Ornamentals Petunia axillaris Plant architecture Plant compactness, dwarfing Plants (botany) Breeding Plants Petunia, a commercially important ornamental plant worldwide, has been subjected to breeding programs that have yielded a high number of varieties. One of the key factors in the commercial value of these varieties is plant compactness. Currently, compact petunias are obtained through the application of expensive, harmful and short-lasting chemicals. To avoid the use of these chemicals, transgenic plants that over-express dwarf-inducing genes have been recently proposed as an alternative, but the current legislation regarding transgenic plants restricts their commercialization. In this work, we studied the effect of polyploidization in the plant architecture of Petunia axillaris, an Argentine native petunia. We developed a new polyploidization protocol that consisted in culturing petunia leaves in RL medium (MS medium supplemented with 30 g l−1 sucrose, 1 mg l−1 BA and 0.2 mg l−1 IAA) supplemented with 0.2 g l−1 colchicine for 15 days. This protocol allowed the regeneration of stable autotetraploid petunias (polyploidization rate: 29.0 ± 8,2%), which were 54% more compact than the diploid ones. Furthermore, they exhibited no variations in agronomical traits compared to the initial genotypes, except for a short delay in blooming. These autotetraploid plants can be used in different breeding programs and the polyploidization method developed can be tested in others cultivars of the genus Petunia for the same purpose. © 2017, Springer Science+Business Media Dordrecht. Fil:Veléz, C.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676857_v129_n1_p61_Regalado http://hdl.handle.net/20.500.12110/paper_01676857_v129_n1_p61_Regalado
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Autotetraploids Colchicine Organogenesis Ornamentals Petunia axillaris Plant architecture Plant compactness, dwarfing Chromosomes Autotetraploids Colchicine Organogenesis Ornamentals Petunia axillaris Plant architecture Plant compactness, dwarfing Plants (botany) Breeding Plants
spellingShingle	Autotetraploids Colchicine Organogenesis Ornamentals Petunia axillaris Plant architecture Plant compactness, dwarfing Chromosomes Autotetraploids Colchicine Organogenesis Ornamentals Petunia axillaris Plant architecture Plant compactness, dwarfing Plants (botany) Breeding Plants Vélez, Carlos Guillermo Production of compact petunias through polyploidization
topic_facet	Autotetraploids Colchicine Organogenesis Ornamentals Petunia axillaris Plant architecture Plant compactness, dwarfing Chromosomes Autotetraploids Colchicine Organogenesis Ornamentals Petunia axillaris Plant architecture Plant compactness, dwarfing Plants (botany) Breeding Plants
description	Petunia, a commercially important ornamental plant worldwide, has been subjected to breeding programs that have yielded a high number of varieties. One of the key factors in the commercial value of these varieties is plant compactness. Currently, compact petunias are obtained through the application of expensive, harmful and short-lasting chemicals. To avoid the use of these chemicals, transgenic plants that over-express dwarf-inducing genes have been recently proposed as an alternative, but the current legislation regarding transgenic plants restricts their commercialization. In this work, we studied the effect of polyploidization in the plant architecture of Petunia axillaris, an Argentine native petunia. We developed a new polyploidization protocol that consisted in culturing petunia leaves in RL medium (MS medium supplemented with 30 g l−1 sucrose, 1 mg l−1 BA and 0.2 mg l−1 IAA) supplemented with 0.2 g l−1 colchicine for 15 days. This protocol allowed the regeneration of stable autotetraploid petunias (polyploidization rate: 29.0 ± 8,2%), which were 54% more compact than the diploid ones. Furthermore, they exhibited no variations in agronomical traits compared to the initial genotypes, except for a short delay in blooming. These autotetraploid plants can be used in different breeding programs and the polyploidization method developed can be tested in others cultivars of the genus Petunia for the same purpose. © 2017, Springer Science+Business Media Dordrecht.
author	Vélez, Carlos Guillermo
author_facet	Vélez, Carlos Guillermo
author_sort	Vélez, Carlos Guillermo
title	Production of compact petunias through polyploidization
title_short	Production of compact petunias through polyploidization
title_full	Production of compact petunias through polyploidization
title_fullStr	Production of compact petunias through polyploidization
title_full_unstemmed	Production of compact petunias through polyploidization
title_sort	production of compact petunias through polyploidization
publishDate	2017
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676857_v129_n1_p61_Regalado http://hdl.handle.net/20.500.12110/paper_01676857_v129_n1_p61_Regalado
work_keys_str_mv	AT velezcarlosguillermo productionofcompactpetuniasthroughpolyploidization
_version_	1768542592685834240

Production of compact petunias through polyploidization

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