Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells

Since apoptosis appeared to be related to neurodegenerative processes, neuroprotection has been involved in investigation of therapeutic approaches focused upon pharmacological agents to prevent neuronal programmed cell death. In this regard, erythropoietin (Epo) seems to play a critical role. The p...

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Autores principales: Pregi, Nicolás, Vittori, Daniela Cecilia, Pérez, Gladys Mabel, Pérez Leirós, Claudia, Nesse, Alcira Beatriz
Publicado: 2006
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674889_v1763_n2_p238_Pregi
http://hdl.handle.net/20.500.12110/paper_01674889_v1763_n2_p238_Pregi
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spelling paper:paper_01674889_v1763_n2_p238_Pregi2023-06-08T15:16:28Z Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells Pregi, Nicolás Vittori, Daniela Cecilia Pérez, Gladys Mabel Pérez Leirós, Claudia Nesse, Alcira Beatriz Apoptosis Bcl-X Erythropoietin Neuroprotection SH-SY5Y cell Staurosporine erythropoietin protein bcl xl staurosporine apoptosis article cell death cell differentiation cell growth cell structure controlled study human human cell immunoprecipitation neurite neuroblastoma cell priority journal reverse transcription polymerase chain reaction Western blotting Apoptosis bcl-X Protein Blotting, Western Cell Differentiation Dose-Response Relationship, Drug Electrophoresis Enzyme Inhibitors Erythropoietin Erythropoietin, Recombinant Humans Kinetics Microscopy, Fluorescence Neuroblastoma Precipitin Tests Receptors, Erythropoietin Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Staurosporine Since apoptosis appeared to be related to neurodegenerative processes, neuroprotection has been involved in investigation of therapeutic approaches focused upon pharmacological agents to prevent neuronal programmed cell death. In this regard, erythropoietin (Epo) seems to play a critical role. The present work was focused on the study of the Epo protective effect upon human neuroblastoma SH-SY5Y cells subjected to differentiation by staurosporine. Under this condition, profuse neurite outgrowth was accompanied by programmed cell death (35% of apoptotic cells by Hoechst assay, showing characteristic DNA ladder pattern). A previous treatment with recombinant human Epo (rHuEpo) increased the expression of the specific receptor for Epo while prevented apoptosis. Simultaneously, morphological changes in neurite elongation and interconnection induced by staurosporine were blocked by Epo. These Epo effects proved to be associated to the induction of Bcl-xL at the mRNA and protein levels (RT-PCR and Western blot after immunoprecipitation) and were mediated by activation of pathways inhibited by wortmannin. In conclusion, the fact that both events induced by staurosporine, cell apoptosis and differentiation, were prevented in SH-SY5Y cells previously exposed to rHuEpo suggests interrelated signaling pathways triggered by the Epo/EpoR interaction. © 2005 Elsevier B.V. All rights reserved. Fil:Pregi, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vittori, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pérez, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Leirós, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Nesse, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674889_v1763_n2_p238_Pregi http://hdl.handle.net/20.500.12110/paper_01674889_v1763_n2_p238_Pregi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Apoptosis
Bcl-X
Erythropoietin
Neuroprotection
SH-SY5Y cell
Staurosporine
erythropoietin
protein bcl xl
staurosporine
apoptosis
article
cell death
cell differentiation
cell growth
cell structure
controlled study
human
human cell
immunoprecipitation
neurite
neuroblastoma cell
priority journal
reverse transcription polymerase chain reaction
Western blotting
Apoptosis
bcl-X Protein
Blotting, Western
Cell Differentiation
Dose-Response Relationship, Drug
Electrophoresis
Enzyme Inhibitors
Erythropoietin
Erythropoietin, Recombinant
Humans
Kinetics
Microscopy, Fluorescence
Neuroblastoma
Precipitin Tests
Receptors, Erythropoietin
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Staurosporine
spellingShingle Apoptosis
Bcl-X
Erythropoietin
Neuroprotection
SH-SY5Y cell
Staurosporine
erythropoietin
protein bcl xl
staurosporine
apoptosis
article
cell death
cell differentiation
cell growth
cell structure
controlled study
human
human cell
immunoprecipitation
neurite
neuroblastoma cell
priority journal
reverse transcription polymerase chain reaction
Western blotting
Apoptosis
bcl-X Protein
Blotting, Western
Cell Differentiation
Dose-Response Relationship, Drug
Electrophoresis
Enzyme Inhibitors
Erythropoietin
Erythropoietin, Recombinant
Humans
Kinetics
Microscopy, Fluorescence
Neuroblastoma
Precipitin Tests
Receptors, Erythropoietin
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Staurosporine
Pregi, Nicolás
Vittori, Daniela Cecilia
Pérez, Gladys Mabel
Pérez Leirós, Claudia
Nesse, Alcira Beatriz
Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells
topic_facet Apoptosis
Bcl-X
Erythropoietin
Neuroprotection
SH-SY5Y cell
Staurosporine
erythropoietin
protein bcl xl
staurosporine
apoptosis
article
cell death
cell differentiation
cell growth
cell structure
controlled study
human
human cell
immunoprecipitation
neurite
neuroblastoma cell
priority journal
reverse transcription polymerase chain reaction
Western blotting
Apoptosis
bcl-X Protein
Blotting, Western
Cell Differentiation
Dose-Response Relationship, Drug
Electrophoresis
Enzyme Inhibitors
Erythropoietin
Erythropoietin, Recombinant
Humans
Kinetics
Microscopy, Fluorescence
Neuroblastoma
Precipitin Tests
Receptors, Erythropoietin
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Staurosporine
description Since apoptosis appeared to be related to neurodegenerative processes, neuroprotection has been involved in investigation of therapeutic approaches focused upon pharmacological agents to prevent neuronal programmed cell death. In this regard, erythropoietin (Epo) seems to play a critical role. The present work was focused on the study of the Epo protective effect upon human neuroblastoma SH-SY5Y cells subjected to differentiation by staurosporine. Under this condition, profuse neurite outgrowth was accompanied by programmed cell death (35% of apoptotic cells by Hoechst assay, showing characteristic DNA ladder pattern). A previous treatment with recombinant human Epo (rHuEpo) increased the expression of the specific receptor for Epo while prevented apoptosis. Simultaneously, morphological changes in neurite elongation and interconnection induced by staurosporine were blocked by Epo. These Epo effects proved to be associated to the induction of Bcl-xL at the mRNA and protein levels (RT-PCR and Western blot after immunoprecipitation) and were mediated by activation of pathways inhibited by wortmannin. In conclusion, the fact that both events induced by staurosporine, cell apoptosis and differentiation, were prevented in SH-SY5Y cells previously exposed to rHuEpo suggests interrelated signaling pathways triggered by the Epo/EpoR interaction. © 2005 Elsevier B.V. All rights reserved.
author Pregi, Nicolás
Vittori, Daniela Cecilia
Pérez, Gladys Mabel
Pérez Leirós, Claudia
Nesse, Alcira Beatriz
author_facet Pregi, Nicolás
Vittori, Daniela Cecilia
Pérez, Gladys Mabel
Pérez Leirós, Claudia
Nesse, Alcira Beatriz
author_sort Pregi, Nicolás
title Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells
title_short Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells
title_full Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells
title_fullStr Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells
title_full_unstemmed Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells
title_sort effect of erythropoietin on staurosporine-induced apoptosis and differentiation of sh-sy5y neuroblastoma cells
publishDate 2006
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674889_v1763_n2_p238_Pregi
http://hdl.handle.net/20.500.12110/paper_01674889_v1763_n2_p238_Pregi
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