Cholinesterases and neurotoxicity as highly sensitive biomarkers for an organophosphate insecticide in a freshwater gastropod (Chilina gibbosa) with low sensitivity carboxylesterases
In the Upper Valley of Río Negro and Río Neuquén in Argentina, agriculture represents the second most important economic activity. Azinphos-methyl has been found in water from this region throughout the year at a maximum concentration of 22.48μgL-1 during the application period. Toxicological studie...
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2013
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v144-145_n_p26_Bianco http://hdl.handle.net/20.500.12110/paper_0166445X_v144-145_n_p26_Bianco |
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paper:paper_0166445X_v144-145_n_p26_Bianco |
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dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antioxidant defenses Azinphos-methyl B-esterases Biomarkers Invertebrates 4 nitrophenyl acetate acetylthiocholine azinphos methyl butyric acid 4 nitrophenyl ester butyrylthiocholine carboxylesterase catalase choline derivative cholinesterase glutathione glutathione transferase nitrogen derivative organophosphate insecticide physostigmine propionylthiocholine superoxide dismutase unclassified drug antioxidant biomarker ecotoxicology enzyme activity gastropod insecticide organophosphate toxicity acute toxicity animal experiment animal tissue article Chilina gibbosa controlled study enzyme activity enzyme substrate gastropod IC 50 neurotoxicity nonhuman priority journal sensitivity and specificity Argentina Neuquen Basin Rio Negro [Argentina] 1-chloro-2,4-dinitrobenzene 5,5′-dithio-2-bis-nitrobenzoate 50% inhibition concentration acetylthiocholine iodide Antioxidant defenses ASCh Azinphos-methyl B-esterases Biomarkers BSCh butyrylthiocholine iodide carboxylesterase CAT catalase CDNB CE ChE cholinesterase DTNB glutathione glutathione S-transferase GSH GST IC(50) Invertebrates iso-OMPA NOEC OP organophosphate insecticide p-nitrophenyl acetate p-nitrophenyl butyrate p-NPA p-NPB propionylthiocholine iodide PrSCh SOD superoxide dismutase tetraisopropyl pyrophosphoramide the no observed effect concentration Animals Argentina Biological Markers Carboxylic Ester Hydrolases Cholinesterases Environmental Monitoring Gastropoda Insecticides Motor Activity Nervous System Organophosphorus Compounds Water Pollutants, Chemical |
| spellingShingle |
Antioxidant defenses Azinphos-methyl B-esterases Biomarkers Invertebrates 4 nitrophenyl acetate acetylthiocholine azinphos methyl butyric acid 4 nitrophenyl ester butyrylthiocholine carboxylesterase catalase choline derivative cholinesterase glutathione glutathione transferase nitrogen derivative organophosphate insecticide physostigmine propionylthiocholine superoxide dismutase unclassified drug antioxidant biomarker ecotoxicology enzyme activity gastropod insecticide organophosphate toxicity acute toxicity animal experiment animal tissue article Chilina gibbosa controlled study enzyme activity enzyme substrate gastropod IC 50 neurotoxicity nonhuman priority journal sensitivity and specificity Argentina Neuquen Basin Rio Negro [Argentina] 1-chloro-2,4-dinitrobenzene 5,5′-dithio-2-bis-nitrobenzoate 50% inhibition concentration acetylthiocholine iodide Antioxidant defenses ASCh Azinphos-methyl B-esterases Biomarkers BSCh butyrylthiocholine iodide carboxylesterase CAT catalase CDNB CE ChE cholinesterase DTNB glutathione glutathione S-transferase GSH GST IC(50) Invertebrates iso-OMPA NOEC OP organophosphate insecticide p-nitrophenyl acetate p-nitrophenyl butyrate p-NPA p-NPB propionylthiocholine iodide PrSCh SOD superoxide dismutase tetraisopropyl pyrophosphoramide the no observed effect concentration Animals Argentina Biological Markers Carboxylic Ester Hydrolases Cholinesterases Environmental Monitoring Gastropoda Insecticides Motor Activity Nervous System Organophosphorus Compounds Water Pollutants, Chemical Yusseppone, María Soledad Luquet, Carlos Marcelo Ríos de Molina, María del Carmen Kristoff, Gisela Cholinesterases and neurotoxicity as highly sensitive biomarkers for an organophosphate insecticide in a freshwater gastropod (Chilina gibbosa) with low sensitivity carboxylesterases |
| topic_facet |
Antioxidant defenses Azinphos-methyl B-esterases Biomarkers Invertebrates 4 nitrophenyl acetate acetylthiocholine azinphos methyl butyric acid 4 nitrophenyl ester butyrylthiocholine carboxylesterase catalase choline derivative cholinesterase glutathione glutathione transferase nitrogen derivative organophosphate insecticide physostigmine propionylthiocholine superoxide dismutase unclassified drug antioxidant biomarker ecotoxicology enzyme activity gastropod insecticide organophosphate toxicity acute toxicity animal experiment animal tissue article Chilina gibbosa controlled study enzyme activity enzyme substrate gastropod IC 50 neurotoxicity nonhuman priority journal sensitivity and specificity Argentina Neuquen Basin Rio Negro [Argentina] 1-chloro-2,4-dinitrobenzene 5,5′-dithio-2-bis-nitrobenzoate 50% inhibition concentration acetylthiocholine iodide Antioxidant defenses ASCh Azinphos-methyl B-esterases Biomarkers BSCh butyrylthiocholine iodide carboxylesterase CAT catalase CDNB CE ChE cholinesterase DTNB glutathione glutathione S-transferase GSH GST IC(50) Invertebrates iso-OMPA NOEC OP organophosphate insecticide p-nitrophenyl acetate p-nitrophenyl butyrate p-NPA p-NPB propionylthiocholine iodide PrSCh SOD superoxide dismutase tetraisopropyl pyrophosphoramide the no observed effect concentration Animals Argentina Biological Markers Carboxylic Ester Hydrolases Cholinesterases Environmental Monitoring Gastropoda Insecticides Motor Activity Nervous System Organophosphorus Compounds Water Pollutants, Chemical |
| description |
In the Upper Valley of Río Negro and Río Neuquén in Argentina, agriculture represents the second most important economic activity. Azinphos-methyl has been found in water from this region throughout the year at a maximum concentration of 22.48μgL-1 during the application period. Toxicological studies on local non-target species have been performed mostly on vertebrates, while mollusks, which could be more sensitive, have not been studied so far. This work aims to characterize cholinesterase (ChE) and carboxilesterase (CE) activities of Chilina gibbosa, a freshwater gastropod native to southern Argentina and Chile. These enzymes, together with neurotoxicity signals, are evaluated herein after as sensitive biomarkers of exposure to azinphos-methyl at environmentally relevant concentrations. Effects of azinphos-methyl on antioxidant defenses: glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST) are also studied in order to complete a set of biomarkers with different sensitivity and specificity, to propose C. gibbosa as a sentinel species. The highest specific activity was obtained with acetylthiocholine as substrate, followed by propionylthiocholine (83% in comparison to acetylthiocholine) and butyrylthiocholine (19%).The lowest Km and the highest efficiency for ChE were obtained with acetylthiocholine. Regarding CEs activities, a higher efficiency was obtained with p-nitrophenyl butyrate than with p-nitrophenyl acetate. Eserine produced significant inhibition of ChE activity (81% with 0.001mM and 98% with 1mM) while iso-OMPA did not produce any significant effect on ChE. Our results show that C. gibbosa ChE is very sensitive to azinphos-methyl (CI50 0.02μgL-1) while CEs are inhibited at higher concentrations (CI50 1000μgL-1). CEs have been reported to be more sensitive to OPs than ChEs in most of the aquatic invertebrates protecting the organisms from neurotoxic effects. In contrast, C. gibbosa, has ChE which are much more sensitive to azinphos-methyl than CEs and shows marked signs of neurotoxicity. Regarding antioxidant defenses, GSH levels were significantly increased by 0.02 and 20μgL-1 azinphos-methyl (80 and 103%, respectively), CAT activity was increased 85% only at 0.02μgL-1 and SOD and GST did not show any significant response. Since ChE activity, neurotoxicity signs, GSH and CAT are sensitive biomarkers of acute exposure to azinphos-methyl at environmental concentrations C. gibbosa could be included as sentinel species in monitoring programs of pesticide hazard in regions of Argentina and Chile. © 2013 Elsevier B.V. |
| author |
Yusseppone, María Soledad Luquet, Carlos Marcelo Ríos de Molina, María del Carmen Kristoff, Gisela |
| author_facet |
Yusseppone, María Soledad Luquet, Carlos Marcelo Ríos de Molina, María del Carmen Kristoff, Gisela |
| author_sort |
Yusseppone, María Soledad |
| title |
Cholinesterases and neurotoxicity as highly sensitive biomarkers for an organophosphate insecticide in a freshwater gastropod (Chilina gibbosa) with low sensitivity carboxylesterases |
| title_short |
Cholinesterases and neurotoxicity as highly sensitive biomarkers for an organophosphate insecticide in a freshwater gastropod (Chilina gibbosa) with low sensitivity carboxylesterases |
| title_full |
Cholinesterases and neurotoxicity as highly sensitive biomarkers for an organophosphate insecticide in a freshwater gastropod (Chilina gibbosa) with low sensitivity carboxylesterases |
| title_fullStr |
Cholinesterases and neurotoxicity as highly sensitive biomarkers for an organophosphate insecticide in a freshwater gastropod (Chilina gibbosa) with low sensitivity carboxylesterases |
| title_full_unstemmed |
Cholinesterases and neurotoxicity as highly sensitive biomarkers for an organophosphate insecticide in a freshwater gastropod (Chilina gibbosa) with low sensitivity carboxylesterases |
| title_sort |
cholinesterases and neurotoxicity as highly sensitive biomarkers for an organophosphate insecticide in a freshwater gastropod (chilina gibbosa) with low sensitivity carboxylesterases |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v144-145_n_p26_Bianco http://hdl.handle.net/20.500.12110/paper_0166445X_v144-145_n_p26_Bianco |
| work_keys_str_mv |
AT yussepponemariasoledad cholinesterasesandneurotoxicityashighlysensitivebiomarkersforanorganophosphateinsecticideinafreshwatergastropodchilinagibbosawithlowsensitivitycarboxylesterases AT luquetcarlosmarcelo cholinesterasesandneurotoxicityashighlysensitivebiomarkersforanorganophosphateinsecticideinafreshwatergastropodchilinagibbosawithlowsensitivitycarboxylesterases AT riosdemolinamariadelcarmen cholinesterasesandneurotoxicityashighlysensitivebiomarkersforanorganophosphateinsecticideinafreshwatergastropodchilinagibbosawithlowsensitivitycarboxylesterases AT kristoffgisela cholinesterasesandneurotoxicityashighlysensitivebiomarkersforanorganophosphateinsecticideinafreshwatergastropodchilinagibbosawithlowsensitivitycarboxylesterases |
| _version_ |
1768545968136912896 |
| spelling |
paper:paper_0166445X_v144-145_n_p26_Bianco2023-06-08T15:15:47Z Cholinesterases and neurotoxicity as highly sensitive biomarkers for an organophosphate insecticide in a freshwater gastropod (Chilina gibbosa) with low sensitivity carboxylesterases Yusseppone, María Soledad Luquet, Carlos Marcelo Ríos de Molina, María del Carmen Kristoff, Gisela Antioxidant defenses Azinphos-methyl B-esterases Biomarkers Invertebrates 4 nitrophenyl acetate acetylthiocholine azinphos methyl butyric acid 4 nitrophenyl ester butyrylthiocholine carboxylesterase catalase choline derivative cholinesterase glutathione glutathione transferase nitrogen derivative organophosphate insecticide physostigmine propionylthiocholine superoxide dismutase unclassified drug antioxidant biomarker ecotoxicology enzyme activity gastropod insecticide organophosphate toxicity acute toxicity animal experiment animal tissue article Chilina gibbosa controlled study enzyme activity enzyme substrate gastropod IC 50 neurotoxicity nonhuman priority journal sensitivity and specificity Argentina Neuquen Basin Rio Negro [Argentina] 1-chloro-2,4-dinitrobenzene 5,5′-dithio-2-bis-nitrobenzoate 50% inhibition concentration acetylthiocholine iodide Antioxidant defenses ASCh Azinphos-methyl B-esterases Biomarkers BSCh butyrylthiocholine iodide carboxylesterase CAT catalase CDNB CE ChE cholinesterase DTNB glutathione glutathione S-transferase GSH GST IC(50) Invertebrates iso-OMPA NOEC OP organophosphate insecticide p-nitrophenyl acetate p-nitrophenyl butyrate p-NPA p-NPB propionylthiocholine iodide PrSCh SOD superoxide dismutase tetraisopropyl pyrophosphoramide the no observed effect concentration Animals Argentina Biological Markers Carboxylic Ester Hydrolases Cholinesterases Environmental Monitoring Gastropoda Insecticides Motor Activity Nervous System Organophosphorus Compounds Water Pollutants, Chemical In the Upper Valley of Río Negro and Río Neuquén in Argentina, agriculture represents the second most important economic activity. Azinphos-methyl has been found in water from this region throughout the year at a maximum concentration of 22.48μgL-1 during the application period. Toxicological studies on local non-target species have been performed mostly on vertebrates, while mollusks, which could be more sensitive, have not been studied so far. This work aims to characterize cholinesterase (ChE) and carboxilesterase (CE) activities of Chilina gibbosa, a freshwater gastropod native to southern Argentina and Chile. These enzymes, together with neurotoxicity signals, are evaluated herein after as sensitive biomarkers of exposure to azinphos-methyl at environmentally relevant concentrations. Effects of azinphos-methyl on antioxidant defenses: glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST) are also studied in order to complete a set of biomarkers with different sensitivity and specificity, to propose C. gibbosa as a sentinel species. The highest specific activity was obtained with acetylthiocholine as substrate, followed by propionylthiocholine (83% in comparison to acetylthiocholine) and butyrylthiocholine (19%).The lowest Km and the highest efficiency for ChE were obtained with acetylthiocholine. Regarding CEs activities, a higher efficiency was obtained with p-nitrophenyl butyrate than with p-nitrophenyl acetate. Eserine produced significant inhibition of ChE activity (81% with 0.001mM and 98% with 1mM) while iso-OMPA did not produce any significant effect on ChE. Our results show that C. gibbosa ChE is very sensitive to azinphos-methyl (CI50 0.02μgL-1) while CEs are inhibited at higher concentrations (CI50 1000μgL-1). CEs have been reported to be more sensitive to OPs than ChEs in most of the aquatic invertebrates protecting the organisms from neurotoxic effects. In contrast, C. gibbosa, has ChE which are much more sensitive to azinphos-methyl than CEs and shows marked signs of neurotoxicity. Regarding antioxidant defenses, GSH levels were significantly increased by 0.02 and 20μgL-1 azinphos-methyl (80 and 103%, respectively), CAT activity was increased 85% only at 0.02μgL-1 and SOD and GST did not show any significant response. Since ChE activity, neurotoxicity signs, GSH and CAT are sensitive biomarkers of acute exposure to azinphos-methyl at environmental concentrations C. gibbosa could be included as sentinel species in monitoring programs of pesticide hazard in regions of Argentina and Chile. © 2013 Elsevier B.V. Fil:Yusseppone, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luquet, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ríos de Molina, M.D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kristoff, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v144-145_n_p26_Bianco http://hdl.handle.net/20.500.12110/paper_0166445X_v144-145_n_p26_Bianco |