Aesculus hippocastanum L. seed extract shows virucidal and antiviral activities against respiratory syncytial virus (RSV) and reduces lung inflammation in vivo
Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract disease and bronchiolitis in children worldwide. No vaccine or specific, effective treatment is currently available. β-escin is one of the main bioactive constituents of Aesculus hippocastanum L. (Hippocastanaceae) seed...
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2019
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v164_n_p1_Salinas http://hdl.handle.net/20.500.12110/paper_01663542_v164_n_p1_Salinas |
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paper:paper_01663542_v164_n_p1_Salinas2023-06-08T15:15:39Z Aesculus hippocastanum L. seed extract shows virucidal and antiviral activities against respiratory syncytial virus (RSV) and reduces lung inflammation in vivo Aesculus hippocastanum Antiviral Immunomodulatory Medicinal plants Respiratory syncytial virus (RSV) β-escin Aesculus hippocastanum extract beta actin beta escin gamma interferon immunoglobulin enhancer binding protein interleukin 17 interleukin 4 interleukin 6 interleukin 8 transcription factor AP 1 tumor necrosis factor virus RNA Aesculus hippocastanum animal cell animal experiment animal model animal tissue antiviral activity Article controlled study epithelium cell human human cell immunomodulation in vitro study lung epithelium lung parenchyma macrophage mouse nonhuman plant seed priority journal respiratory syncytial virus infection respiratory tract inflammation RNA replication signal transduction Th2 cell treatment duration Vero cell line virucidal activity virus infectivity virus load Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract disease and bronchiolitis in children worldwide. No vaccine or specific, effective treatment is currently available. β-escin is one of the main bioactive constituents of Aesculus hippocastanum L. (Hippocastanaceae) seed extract (AH), and both β-escin and AH have demonstrated a beneficial role in clinical therapy because of their anti-edematous, anti-inflammatory and antioxidative effects. Besides, we have reported that β-escin and AH show virucidal, antiviral and immunomodulatory activities against the enveloped viruses HSV-1, VSV and Dengue virus in vitro. In this study, we demonstrate that β-escin and AH have virucidal and antiviral activities against RSV, as well as NF-κB, AP-1 and cytokine modulating activities in RSV infected epithelial and macrophage cell lines in vitro. Besides, in a murine model of pulmonary RSV infection, AH treatment improves the course of acute disease, evidenced by decreased weight loss, reduced RSV lung titers, and attenuated airway inflammation. In contrast, even though β-escin showed, similarly to AH, antiviral and immunomodulatory properties in vitro, it neither reduces viral titers nor attenuates lung injury in vivo. Thus, our data demonstrate that AH restrains RSV disease through antiviral and immunomodulatory effect. © 2019 Elsevier B.V. 2019 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v164_n_p1_Salinas http://hdl.handle.net/20.500.12110/paper_01663542_v164_n_p1_Salinas |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Aesculus hippocastanum Antiviral Immunomodulatory Medicinal plants Respiratory syncytial virus (RSV) β-escin Aesculus hippocastanum extract beta actin beta escin gamma interferon immunoglobulin enhancer binding protein interleukin 17 interleukin 4 interleukin 6 interleukin 8 transcription factor AP 1 tumor necrosis factor virus RNA Aesculus hippocastanum animal cell animal experiment animal model animal tissue antiviral activity Article controlled study epithelium cell human human cell immunomodulation in vitro study lung epithelium lung parenchyma macrophage mouse nonhuman plant seed priority journal respiratory syncytial virus infection respiratory tract inflammation RNA replication signal transduction Th2 cell treatment duration Vero cell line virucidal activity virus infectivity virus load |
| spellingShingle |
Aesculus hippocastanum Antiviral Immunomodulatory Medicinal plants Respiratory syncytial virus (RSV) β-escin Aesculus hippocastanum extract beta actin beta escin gamma interferon immunoglobulin enhancer binding protein interleukin 17 interleukin 4 interleukin 6 interleukin 8 transcription factor AP 1 tumor necrosis factor virus RNA Aesculus hippocastanum animal cell animal experiment animal model animal tissue antiviral activity Article controlled study epithelium cell human human cell immunomodulation in vitro study lung epithelium lung parenchyma macrophage mouse nonhuman plant seed priority journal respiratory syncytial virus infection respiratory tract inflammation RNA replication signal transduction Th2 cell treatment duration Vero cell line virucidal activity virus infectivity virus load Aesculus hippocastanum L. seed extract shows virucidal and antiviral activities against respiratory syncytial virus (RSV) and reduces lung inflammation in vivo |
| topic_facet |
Aesculus hippocastanum Antiviral Immunomodulatory Medicinal plants Respiratory syncytial virus (RSV) β-escin Aesculus hippocastanum extract beta actin beta escin gamma interferon immunoglobulin enhancer binding protein interleukin 17 interleukin 4 interleukin 6 interleukin 8 transcription factor AP 1 tumor necrosis factor virus RNA Aesculus hippocastanum animal cell animal experiment animal model animal tissue antiviral activity Article controlled study epithelium cell human human cell immunomodulation in vitro study lung epithelium lung parenchyma macrophage mouse nonhuman plant seed priority journal respiratory syncytial virus infection respiratory tract inflammation RNA replication signal transduction Th2 cell treatment duration Vero cell line virucidal activity virus infectivity virus load |
| description |
Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract disease and bronchiolitis in children worldwide. No vaccine or specific, effective treatment is currently available. β-escin is one of the main bioactive constituents of Aesculus hippocastanum L. (Hippocastanaceae) seed extract (AH), and both β-escin and AH have demonstrated a beneficial role in clinical therapy because of their anti-edematous, anti-inflammatory and antioxidative effects. Besides, we have reported that β-escin and AH show virucidal, antiviral and immunomodulatory activities against the enveloped viruses HSV-1, VSV and Dengue virus in vitro. In this study, we demonstrate that β-escin and AH have virucidal and antiviral activities against RSV, as well as NF-κB, AP-1 and cytokine modulating activities in RSV infected epithelial and macrophage cell lines in vitro. Besides, in a murine model of pulmonary RSV infection, AH treatment improves the course of acute disease, evidenced by decreased weight loss, reduced RSV lung titers, and attenuated airway inflammation. In contrast, even though β-escin showed, similarly to AH, antiviral and immunomodulatory properties in vitro, it neither reduces viral titers nor attenuates lung injury in vivo. Thus, our data demonstrate that AH restrains RSV disease through antiviral and immunomodulatory effect. © 2019 Elsevier B.V. |
| title |
Aesculus hippocastanum L. seed extract shows virucidal and antiviral activities against respiratory syncytial virus (RSV) and reduces lung inflammation in vivo |
| title_short |
Aesculus hippocastanum L. seed extract shows virucidal and antiviral activities against respiratory syncytial virus (RSV) and reduces lung inflammation in vivo |
| title_full |
Aesculus hippocastanum L. seed extract shows virucidal and antiviral activities against respiratory syncytial virus (RSV) and reduces lung inflammation in vivo |
| title_fullStr |
Aesculus hippocastanum L. seed extract shows virucidal and antiviral activities against respiratory syncytial virus (RSV) and reduces lung inflammation in vivo |
| title_full_unstemmed |
Aesculus hippocastanum L. seed extract shows virucidal and antiviral activities against respiratory syncytial virus (RSV) and reduces lung inflammation in vivo |
| title_sort |
aesculus hippocastanum l. seed extract shows virucidal and antiviral activities against respiratory syncytial virus (rsv) and reduces lung inflammation in vivo |
| publishDate |
2019 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v164_n_p1_Salinas http://hdl.handle.net/20.500.12110/paper_01663542_v164_n_p1_Salinas |
| _version_ |
1768543319716003840 |