Aesculus hippocastanum L. seed extract shows virucidal and antiviral activities against respiratory syncytial virus (RSV) and reduces lung inflammation in vivo

Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract disease and bronchiolitis in children worldwide. No vaccine or specific, effective treatment is currently available. β-escin is one of the main bioactive constituents of Aesculus hippocastanum L. (Hippocastanaceae) seed...

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Detalles Bibliográficos
Publicado: 2019
Materias:
Aesculus hippocastanum
Antiviral
Immunomodulatory
Medicinal plants
Respiratory syncytial virus (RSV)
β-escin
Aesculus hippocastanum extract
beta actin
beta escin
gamma interferon
immunoglobulin enhancer binding protein
interleukin 17
interleukin 4
interleukin 6
interleukin 8
transcription factor AP 1
tumor necrosis factor
virus RNA
animal cell
animal experiment
animal model
animal tissue
antiviral activity
Article
controlled study
epithelium cell
human
human cell
immunomodulation
in vitro study
lung epithelium
lung parenchyma
macrophage
mouse
nonhuman
plant seed
priority journal
respiratory syncytial virus infection
respiratory tract inflammation
RNA replication
signal transduction
Th2 cell
treatment duration
Vero cell line
virucidal activity
virus infectivity
virus load
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v164_n_p1_Salinas
http://hdl.handle.net/20.500.12110/paper_01663542_v164_n_p1_Salinas
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract disease and bronchiolitis in children worldwide. No vaccine or specific, effective treatment is currently available. β-escin is one of the main bioactive constituents of Aesculus hippocastanum L. (Hippocastanaceae) seed extract (AH), and both β-escin and AH have demonstrated a beneficial role in clinical therapy because of their anti-edematous, anti-inflammatory and antioxidative effects. Besides, we have reported that β-escin and AH show virucidal, antiviral and immunomodulatory activities against the enveloped viruses HSV-1, VSV and Dengue virus in vitro. In this study, we demonstrate that β-escin and AH have virucidal and antiviral activities against RSV, as well as NF-κB, AP-1 and cytokine modulating activities in RSV infected epithelial and macrophage cell lines in vitro. Besides, in a murine model of pulmonary RSV infection, AH treatment improves the course of acute disease, evidenced by decreased weight loss, reduced RSV lung titers, and attenuated airway inflammation. In contrast, even though β-escin showed, similarly to AH, antiviral and immunomodulatory properties in vitro, it neither reduces viral titers nor attenuates lung injury in vivo. Thus, our data demonstrate that AH restrains RSV disease through antiviral and immunomodulatory effect. © 2019 Elsevier B.V.

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