High molecular weight components containing N-linked oligosaccharides of Ascaris suum extract inhibit the dendritic cells activation through DC-SIGN and MR
Helminths, as well as their secretory/excretory products, induce a tolerogenic immune microenvironment. High molecular weight components (PI) from Ascaris suum extract down-modulate the immune response against ovalbumin (OVA). The PI exerts direct effect on dendritic cells (DCs) independent of TLR 2...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01615890_v87_n_p33_Favoretto http://hdl.handle.net/20.500.12110/paper_01615890_v87_n_p33_Favoretto |
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paper:paper_01615890_v87_n_p33_Favoretto2023-06-08T15:13:30Z High molecular weight components containing N-linked oligosaccharides of Ascaris suum extract inhibit the dendritic cells activation through DC-SIGN and MR Casabuono, Adriana Cristina Couto, Alicia Susana Ascaris suum C-type lectin receptors DC-Dendritic Cell DCSIGN-Dendritic cell specifc ICAM-3 grabbing non-integrin MR-Mannose receptor PI - High molecular weight components from Ascaris suum extract asparagine linked oligosaccharide CD209 antigen Freund adjuvant granulocyte macrophage colony stimulating factor high molecular weight component interleukin 4 mannan mannose receptor natural products and their synthetic derivatives ovalbumin unclassified drug lectin oligosaccharide phosphorylcholine animal cell animal experiment Article Ascaris suum binding affinity binding assay bound fraction Canavalia ensiformis cell activation cell surface cell suspension confocal microscopy dendritic cell flow cytometry immunization in vitro study internalization lectin binding lymphocyte proliferation male matrix assisted laser desorption ionization time of flight mass spectrometry molecular weight mouse nonhuman priority journal protein content purification retention time T lymphocyte animal Ascaris suum Bagg albino mouse cell proliferation chemistry dendritic cell drug effects immunology lymphocyte activation Animals Ascaris suum Cell Proliferation Dendritic Cells Lectins, C-Type Lymphocyte Activation Male Mannans Mice Mice, Inbred BALB C Molecular Weight Oligosaccharides Ovalbumin Phosphorylcholine T-Lymphocytes Helminths, as well as their secretory/excretory products, induce a tolerogenic immune microenvironment. High molecular weight components (PI) from Ascaris suum extract down-modulate the immune response against ovalbumin (OVA). The PI exerts direct effect on dendritic cells (DCs) independent of TLR 2, 4 and MyD88 molecule and, thus, decreases the T lymphocytes response. Here, we studied the glycoconjugates in PI and the role of C-type lectin receptors (CLRs), DC-SIGN and MR, in the modulation of DCs activity. Our data showed the presence of glycoconjugates with high mannose- and complex-type N-linked oligosaccharide chains and phosphorylcholine residues on PI. In addition, these N-linked glycoconjugates inhibited the DCs maturation induced by LPS. The binding and internalization of PI-Alexa were decreased on DCs previously incubated with mannan, anti-DC-SIGN and/or anti-MR antibodies. In agreement with this, the incubation of DCs with mannan, anti-DC-SIGN and/or anti-MR antibodies abolished the down-modulatory effect of PI on these cells. It was also observed that the blockage of CLRs, DC-SIGN and MR on DCs reverted the inhibitory effect of PI in in vitro T cells proliferation. Therefore, our data show the involvement of DC-SIGN and MR in the recognition and consequent modulatory effect of N-glycosylated components of PI on DCs. © 2017 Elsevier Ltd Fil:Casabuono, A.A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01615890_v87_n_p33_Favoretto http://hdl.handle.net/20.500.12110/paper_01615890_v87_n_p33_Favoretto |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Ascaris suum C-type lectin receptors DC-Dendritic Cell DCSIGN-Dendritic cell specifc ICAM-3 grabbing non-integrin MR-Mannose receptor PI - High molecular weight components from Ascaris suum extract asparagine linked oligosaccharide CD209 antigen Freund adjuvant granulocyte macrophage colony stimulating factor high molecular weight component interleukin 4 mannan mannose receptor natural products and their synthetic derivatives ovalbumin unclassified drug lectin oligosaccharide phosphorylcholine animal cell animal experiment Article Ascaris suum binding affinity binding assay bound fraction Canavalia ensiformis cell activation cell surface cell suspension confocal microscopy dendritic cell flow cytometry immunization in vitro study internalization lectin binding lymphocyte proliferation male matrix assisted laser desorption ionization time of flight mass spectrometry molecular weight mouse nonhuman priority journal protein content purification retention time T lymphocyte animal Ascaris suum Bagg albino mouse cell proliferation chemistry dendritic cell drug effects immunology lymphocyte activation Animals Ascaris suum Cell Proliferation Dendritic Cells Lectins, C-Type Lymphocyte Activation Male Mannans Mice Mice, Inbred BALB C Molecular Weight Oligosaccharides Ovalbumin Phosphorylcholine T-Lymphocytes |
| spellingShingle |
Ascaris suum C-type lectin receptors DC-Dendritic Cell DCSIGN-Dendritic cell specifc ICAM-3 grabbing non-integrin MR-Mannose receptor PI - High molecular weight components from Ascaris suum extract asparagine linked oligosaccharide CD209 antigen Freund adjuvant granulocyte macrophage colony stimulating factor high molecular weight component interleukin 4 mannan mannose receptor natural products and their synthetic derivatives ovalbumin unclassified drug lectin oligosaccharide phosphorylcholine animal cell animal experiment Article Ascaris suum binding affinity binding assay bound fraction Canavalia ensiformis cell activation cell surface cell suspension confocal microscopy dendritic cell flow cytometry immunization in vitro study internalization lectin binding lymphocyte proliferation male matrix assisted laser desorption ionization time of flight mass spectrometry molecular weight mouse nonhuman priority journal protein content purification retention time T lymphocyte animal Ascaris suum Bagg albino mouse cell proliferation chemistry dendritic cell drug effects immunology lymphocyte activation Animals Ascaris suum Cell Proliferation Dendritic Cells Lectins, C-Type Lymphocyte Activation Male Mannans Mice Mice, Inbred BALB C Molecular Weight Oligosaccharides Ovalbumin Phosphorylcholine T-Lymphocytes Casabuono, Adriana Cristina Couto, Alicia Susana High molecular weight components containing N-linked oligosaccharides of Ascaris suum extract inhibit the dendritic cells activation through DC-SIGN and MR |
| topic_facet |
Ascaris suum C-type lectin receptors DC-Dendritic Cell DCSIGN-Dendritic cell specifc ICAM-3 grabbing non-integrin MR-Mannose receptor PI - High molecular weight components from Ascaris suum extract asparagine linked oligosaccharide CD209 antigen Freund adjuvant granulocyte macrophage colony stimulating factor high molecular weight component interleukin 4 mannan mannose receptor natural products and their synthetic derivatives ovalbumin unclassified drug lectin oligosaccharide phosphorylcholine animal cell animal experiment Article Ascaris suum binding affinity binding assay bound fraction Canavalia ensiformis cell activation cell surface cell suspension confocal microscopy dendritic cell flow cytometry immunization in vitro study internalization lectin binding lymphocyte proliferation male matrix assisted laser desorption ionization time of flight mass spectrometry molecular weight mouse nonhuman priority journal protein content purification retention time T lymphocyte animal Ascaris suum Bagg albino mouse cell proliferation chemistry dendritic cell drug effects immunology lymphocyte activation Animals Ascaris suum Cell Proliferation Dendritic Cells Lectins, C-Type Lymphocyte Activation Male Mannans Mice Mice, Inbred BALB C Molecular Weight Oligosaccharides Ovalbumin Phosphorylcholine T-Lymphocytes |
| description |
Helminths, as well as their secretory/excretory products, induce a tolerogenic immune microenvironment. High molecular weight components (PI) from Ascaris suum extract down-modulate the immune response against ovalbumin (OVA). The PI exerts direct effect on dendritic cells (DCs) independent of TLR 2, 4 and MyD88 molecule and, thus, decreases the T lymphocytes response. Here, we studied the glycoconjugates in PI and the role of C-type lectin receptors (CLRs), DC-SIGN and MR, in the modulation of DCs activity. Our data showed the presence of glycoconjugates with high mannose- and complex-type N-linked oligosaccharide chains and phosphorylcholine residues on PI. In addition, these N-linked glycoconjugates inhibited the DCs maturation induced by LPS. The binding and internalization of PI-Alexa were decreased on DCs previously incubated with mannan, anti-DC-SIGN and/or anti-MR antibodies. In agreement with this, the incubation of DCs with mannan, anti-DC-SIGN and/or anti-MR antibodies abolished the down-modulatory effect of PI on these cells. It was also observed that the blockage of CLRs, DC-SIGN and MR on DCs reverted the inhibitory effect of PI in in vitro T cells proliferation. Therefore, our data show the involvement of DC-SIGN and MR in the recognition and consequent modulatory effect of N-glycosylated components of PI on DCs. © 2017 Elsevier Ltd |
| author |
Casabuono, Adriana Cristina Couto, Alicia Susana |
| author_facet |
Casabuono, Adriana Cristina Couto, Alicia Susana |
| author_sort |
Casabuono, Adriana Cristina |
| title |
High molecular weight components containing N-linked oligosaccharides of Ascaris suum extract inhibit the dendritic cells activation through DC-SIGN and MR |
| title_short |
High molecular weight components containing N-linked oligosaccharides of Ascaris suum extract inhibit the dendritic cells activation through DC-SIGN and MR |
| title_full |
High molecular weight components containing N-linked oligosaccharides of Ascaris suum extract inhibit the dendritic cells activation through DC-SIGN and MR |
| title_fullStr |
High molecular weight components containing N-linked oligosaccharides of Ascaris suum extract inhibit the dendritic cells activation through DC-SIGN and MR |
| title_full_unstemmed |
High molecular weight components containing N-linked oligosaccharides of Ascaris suum extract inhibit the dendritic cells activation through DC-SIGN and MR |
| title_sort |
high molecular weight components containing n-linked oligosaccharides of ascaris suum extract inhibit the dendritic cells activation through dc-sign and mr |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01615890_v87_n_p33_Favoretto http://hdl.handle.net/20.500.12110/paper_01615890_v87_n_p33_Favoretto |
| work_keys_str_mv |
AT casabuonoadrianacristina highmolecularweightcomponentscontainingnlinkedoligosaccharidesofascarissuumextractinhibitthedendriticcellsactivationthroughdcsignandmr AT coutoaliciasusana highmolecularweightcomponentscontainingnlinkedoligosaccharidesofascarissuumextractinhibitthedendriticcellsactivationthroughdcsignandmr |
| _version_ |
1768544861163618304 |