Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype

Natural carrageenans of diverse structural types isolated from the red seaweed Gigartina skottsbergii were recently identified as potent and selective inhibitors of herpes simplex virus types 1 and 2 (HSV-1 and -2). The μ/ν-carrageenan 1C3 was tested in vitro for its ability to select resistant vari...

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Autores principales:	Carlucci, María Josefina, Scolaro, Luis Alberto, Damonte, Elsa Beatriz
Publicado:	2002
Materias:	Antiviral Antiviral resistance Carrageenan Herpesvirus Syncytial variant aciclovir antivirus agent carrageenan cyclosporin dextran sulfate glycoprotein heparin melittin polysaccharide animal cell animal experiment animal model antiviral activity article cell fusion concentration response controlled study drug sensitivity Herpes simplex virus 1 Herpes simplex virus 2 human human cell mouse nonhuman phenotype phenotypic variation seaweed Vero cell virogenesis virus inhibition virus plaque virus resistance virus virulence animal cell culture Cercopithecus disease model drug effect genetic variability giant cell herpes simplex pathogenicity physiology virology Animal Antiviral Agents Cercopithecus aethiops Disease Models, Animal Giant Cells Herpes Simplex Herpesvirus 1, Human Human Mice Phenotype Support, Non-U.S. Gov't Tumor Cells, Cultured Variation (Genetics) Vero Cells Animals Humans algae Gigartina Gigartina skottsbergii Herpesviridae Human herpesvirus 1 Simplexvirus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01466615_v68_n1_p92_Carlucci http://hdl.handle.net/20.500.12110/paper_01466615_v68_n1_p92_Carlucci
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01466615_v68_n1_p92_Carlucci
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spelling	paper:paper_01466615_v68_n1_p92_Carlucci2023-06-08T15:12:33Z Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype Carlucci, María Josefina Scolaro, Luis Alberto Damonte, Elsa Beatriz Antiviral Antiviral resistance Carrageenan Herpesvirus Syncytial variant aciclovir antivirus agent carrageenan cyclosporin dextran sulfate glycoprotein heparin melittin polysaccharide antivirus agent carrageenan animal cell animal experiment animal model antiviral activity article cell fusion concentration response controlled study drug sensitivity Herpes simplex virus 1 Herpes simplex virus 2 human human cell mouse nonhuman phenotype phenotypic variation seaweed Vero cell virogenesis virus inhibition virus plaque virus resistance virus virulence animal cell culture Cercopithecus disease model drug effect genetic variability giant cell herpes simplex pathogenicity phenotype physiology virology Animal Antiviral Agents Carrageenan Cercopithecus aethiops Disease Models, Animal Giant Cells Herpes Simplex Herpesvirus 1, Human Human Mice Phenotype Support, Non-U.S. Gov't Tumor Cells, Cultured Variation (Genetics) Vero Cells Animals Cercopithecus aethiops Disease Models, Animal Giant Cells Humans Mice Phenotype Tumor Cells, Cultured Variation (Genetics) Vero Cells algae Gigartina Gigartina skottsbergii Herpesviridae Human herpesvirus 1 Simplexvirus Natural carrageenans of diverse structural types isolated from the red seaweed Gigartina skottsbergii were recently identified as potent and selective inhibitors of herpes simplex virus types 1 and 2 (HSV-1 and -2). The μ/ν-carrageenan 1C3 was tested in vitro for its ability to select resistant variants. After serial passages of HSV-1 strain F in Vero cells in the presence of increasing concentrations of 1C3, viruses emerged that were approximately 2- to 10-fold more resistant to 1C3 inhibition than parental virus; these viruses formed large plaques with an altered syncytial phenotype (1C3-syn). Plaque-purified syncytial variants isolated from passages 13 and 14 have shown variable levels of resistance to 1C3, as well as to the other antiviral carrageenans isolated from G. skottsbergii and to other sulfated polysaccharides with known antiviral activity, such as heparin and dextran sulfate 8000, but all the clones were susceptible to acyclovir. The syn phenotype was not related to polysaccharide resistance. All the 1C3-syn variants formed large syncytia in Vero and CV-1 cells but did not induce fusion in other cell types. The growth efficiency in Vero cells, as well as the virulence for mice by intracerebral or intraperitoneal inoculation of 1C3-syn variants, showed no significant alterations in comparison with the parental virus. The syncytial properties were not affected by cyclosporine or melittin, suggesting that an alteration on glycoprotein gB could be responsible for the syn phenotype induced by 1C3. © 2002 Wiley-Liss, Inc. Fil:Carlucci, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Scolaro, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01466615_v68_n1_p92_Carlucci http://hdl.handle.net/20.500.12110/paper_01466615_v68_n1_p92_Carlucci
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Antiviral Antiviral resistance Carrageenan Herpesvirus Syncytial variant aciclovir antivirus agent carrageenan cyclosporin dextran sulfate glycoprotein heparin melittin polysaccharide antivirus agent carrageenan animal cell animal experiment animal model antiviral activity article cell fusion concentration response controlled study drug sensitivity Herpes simplex virus 1 Herpes simplex virus 2 human human cell mouse nonhuman phenotype phenotypic variation seaweed Vero cell virogenesis virus inhibition virus plaque virus resistance virus virulence animal cell culture Cercopithecus disease model drug effect genetic variability giant cell herpes simplex pathogenicity phenotype physiology virology Animal Antiviral Agents Carrageenan Cercopithecus aethiops Disease Models, Animal Giant Cells Herpes Simplex Herpesvirus 1, Human Human Mice Phenotype Support, Non-U.S. Gov't Tumor Cells, Cultured Variation (Genetics) Vero Cells Animals Cercopithecus aethiops Disease Models, Animal Giant Cells Humans Mice Phenotype Tumor Cells, Cultured Variation (Genetics) Vero Cells algae Gigartina Gigartina skottsbergii Herpesviridae Human herpesvirus 1 Simplexvirus
spellingShingle	Antiviral Antiviral resistance Carrageenan Herpesvirus Syncytial variant aciclovir antivirus agent carrageenan cyclosporin dextran sulfate glycoprotein heparin melittin polysaccharide antivirus agent carrageenan animal cell animal experiment animal model antiviral activity article cell fusion concentration response controlled study drug sensitivity Herpes simplex virus 1 Herpes simplex virus 2 human human cell mouse nonhuman phenotype phenotypic variation seaweed Vero cell virogenesis virus inhibition virus plaque virus resistance virus virulence animal cell culture Cercopithecus disease model drug effect genetic variability giant cell herpes simplex pathogenicity phenotype physiology virology Animal Antiviral Agents Carrageenan Cercopithecus aethiops Disease Models, Animal Giant Cells Herpes Simplex Herpesvirus 1, Human Human Mice Phenotype Support, Non-U.S. Gov't Tumor Cells, Cultured Variation (Genetics) Vero Cells Animals Cercopithecus aethiops Disease Models, Animal Giant Cells Humans Mice Phenotype Tumor Cells, Cultured Variation (Genetics) Vero Cells algae Gigartina Gigartina skottsbergii Herpesviridae Human herpesvirus 1 Simplexvirus Carlucci, María Josefina Scolaro, Luis Alberto Damonte, Elsa Beatriz Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype
topic_facet	Antiviral Antiviral resistance Carrageenan Herpesvirus Syncytial variant aciclovir antivirus agent carrageenan cyclosporin dextran sulfate glycoprotein heparin melittin polysaccharide antivirus agent carrageenan animal cell animal experiment animal model antiviral activity article cell fusion concentration response controlled study drug sensitivity Herpes simplex virus 1 Herpes simplex virus 2 human human cell mouse nonhuman phenotype phenotypic variation seaweed Vero cell virogenesis virus inhibition virus plaque virus resistance virus virulence animal cell culture Cercopithecus disease model drug effect genetic variability giant cell herpes simplex pathogenicity phenotype physiology virology Animal Antiviral Agents Carrageenan Cercopithecus aethiops Disease Models, Animal Giant Cells Herpes Simplex Herpesvirus 1, Human Human Mice Phenotype Support, Non-U.S. Gov't Tumor Cells, Cultured Variation (Genetics) Vero Cells Animals Cercopithecus aethiops Disease Models, Animal Giant Cells Humans Mice Phenotype Tumor Cells, Cultured Variation (Genetics) Vero Cells algae Gigartina Gigartina skottsbergii Herpesviridae Human herpesvirus 1 Simplexvirus
description	Natural carrageenans of diverse structural types isolated from the red seaweed Gigartina skottsbergii were recently identified as potent and selective inhibitors of herpes simplex virus types 1 and 2 (HSV-1 and -2). The μ/ν-carrageenan 1C3 was tested in vitro for its ability to select resistant variants. After serial passages of HSV-1 strain F in Vero cells in the presence of increasing concentrations of 1C3, viruses emerged that were approximately 2- to 10-fold more resistant to 1C3 inhibition than parental virus; these viruses formed large plaques with an altered syncytial phenotype (1C3-syn). Plaque-purified syncytial variants isolated from passages 13 and 14 have shown variable levels of resistance to 1C3, as well as to the other antiviral carrageenans isolated from G. skottsbergii and to other sulfated polysaccharides with known antiviral activity, such as heparin and dextran sulfate 8000, but all the clones were susceptible to acyclovir. The syn phenotype was not related to polysaccharide resistance. All the 1C3-syn variants formed large syncytia in Vero and CV-1 cells but did not induce fusion in other cell types. The growth efficiency in Vero cells, as well as the virulence for mice by intracerebral or intraperitoneal inoculation of 1C3-syn variants, showed no significant alterations in comparison with the parental virus. The syncytial properties were not affected by cyclosporine or melittin, suggesting that an alteration on glycoprotein gB could be responsible for the syn phenotype induced by 1C3. © 2002 Wiley-Liss, Inc.
author	Carlucci, María Josefina Scolaro, Luis Alberto Damonte, Elsa Beatriz
author_facet	Carlucci, María Josefina Scolaro, Luis Alberto Damonte, Elsa Beatriz
author_sort	Carlucci, María Josefina
title	Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype
title_short	Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype
title_full	Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype
title_fullStr	Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype
title_full_unstemmed	Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype
title_sort	herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: lack of correlation between drug susceptibility and syn phenotype
publishDate	2002
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01466615_v68_n1_p92_Carlucci http://hdl.handle.net/20.500.12110/paper_01466615_v68_n1_p92_Carlucci
work_keys_str_mv	AT carluccimariajosefina herpessimplexvirustype1variantsarisingafterselectionwithanantiviralcarrageenanlackofcorrelationbetweendrugsusceptibilityandsynphenotype AT scolaroluisalberto herpessimplexvirustype1variantsarisingafterselectionwithanantiviralcarrageenanlackofcorrelationbetweendrugsusceptibilityandsynphenotype AT damonteelsabeatriz herpessimplexvirustype1variantsarisingafterselectionwithanantiviralcarrageenanlackofcorrelationbetweendrugsusceptibilityandsynphenotype
_version_	1768545044483014656

Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype

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