Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice
PURPOSE. To study the effect of aminoguanidine (AMG), an inhibitor of nitric oxide production, on the ocular infection of Balb/c mice with herpes simplex virus (HSV) type 1 strain F and HSV-2 strain G. METHODS. Animals were treated with different amounts of AMG (0.5, O. 1, and 0.05 mg/mouse) by topi...
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2001
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01460404_v42_n6_p1277_Benencia http://hdl.handle.net/20.500.12110/paper_01460404_v42_n6_p1277_Benencia |
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paper:paper_01460404_v42_n6_p1277_Benencia2023-06-08T15:12:31Z Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice aminoguanidine nitric oxide nitric oxide synthase inhibitor animal experiment animal model animal tissue article controlled study cornea dose response eye infection Herpes simplex virus 1 Herpes simplex virus 2 male mouse mouse strain neutrophil nonhuman priority journal virus titration Administration, Topical Animals Cornea Disease Progression DNA, Viral Dose-Response Relationship, Drug Enzyme Inhibitors Guanidines Herpesvirus 1, Human Herpesvirus 2, Human Keratitis, Herpetic Mice Mice, Inbred BALB C Nitric Oxide Synthase Ophthalmic Solutions Reverse Transcriptase Polymerase Chain Reaction Trigeminal Ganglion PURPOSE. To study the effect of aminoguanidine (AMG), an inhibitor of nitric oxide production, on the ocular infection of Balb/c mice with herpes simplex virus (HSV) type 1 strain F and HSV-2 strain G. METHODS. Animals were treated with different amounts of AMG (0.5, O. 1, and 0.05 mg/mouse) by topical application in the eye from postinfection (PI) days -2 through +5, considering 0 the day of infection. At different PI days, development of herpetic keratitis was evaluated in treated and control mice. RESULTS. Treated animals showed a dose-dependent increase in ocular disease after viral infection, compared with control animals. Viral titers in ocular washings were higher in AMG-treated mice (PI day 2, HSV-1: AMG 0.5 mg, 1.3 × 103 plaque-forming units (PFU)/ml; control, O. 22 × 102 PFU/ml, P < 0.025). At PI day 3, control corneas had only scattered inflammatory cells, whereas those from treated animals showed a conspicuous infiltrate consisting primarily of neutrophils. Viral titers were also higher in brains of treated mice. These animals died earlier and in a greater proportion than control animals (percentage of mortality, PI day 12, HSV-1: AMG 0.5 mg, 40% ± 4%; control, 18% ± 3%, P < 0.05). CONCLUSIONS. These data indicate an inhibitory effect of nitric oxide on HSV ocular infection. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01460404_v42_n6_p1277_Benencia http://hdl.handle.net/20.500.12110/paper_01460404_v42_n6_p1277_Benencia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
aminoguanidine nitric oxide nitric oxide synthase inhibitor animal experiment animal model animal tissue article controlled study cornea dose response eye infection Herpes simplex virus 1 Herpes simplex virus 2 male mouse mouse strain neutrophil nonhuman priority journal virus titration Administration, Topical Animals Cornea Disease Progression DNA, Viral Dose-Response Relationship, Drug Enzyme Inhibitors Guanidines Herpesvirus 1, Human Herpesvirus 2, Human Keratitis, Herpetic Mice Mice, Inbred BALB C Nitric Oxide Synthase Ophthalmic Solutions Reverse Transcriptase Polymerase Chain Reaction Trigeminal Ganglion |
| spellingShingle |
aminoguanidine nitric oxide nitric oxide synthase inhibitor animal experiment animal model animal tissue article controlled study cornea dose response eye infection Herpes simplex virus 1 Herpes simplex virus 2 male mouse mouse strain neutrophil nonhuman priority journal virus titration Administration, Topical Animals Cornea Disease Progression DNA, Viral Dose-Response Relationship, Drug Enzyme Inhibitors Guanidines Herpesvirus 1, Human Herpesvirus 2, Human Keratitis, Herpetic Mice Mice, Inbred BALB C Nitric Oxide Synthase Ophthalmic Solutions Reverse Transcriptase Polymerase Chain Reaction Trigeminal Ganglion Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice |
| topic_facet |
aminoguanidine nitric oxide nitric oxide synthase inhibitor animal experiment animal model animal tissue article controlled study cornea dose response eye infection Herpes simplex virus 1 Herpes simplex virus 2 male mouse mouse strain neutrophil nonhuman priority journal virus titration Administration, Topical Animals Cornea Disease Progression DNA, Viral Dose-Response Relationship, Drug Enzyme Inhibitors Guanidines Herpesvirus 1, Human Herpesvirus 2, Human Keratitis, Herpetic Mice Mice, Inbred BALB C Nitric Oxide Synthase Ophthalmic Solutions Reverse Transcriptase Polymerase Chain Reaction Trigeminal Ganglion |
| description |
PURPOSE. To study the effect of aminoguanidine (AMG), an inhibitor of nitric oxide production, on the ocular infection of Balb/c mice with herpes simplex virus (HSV) type 1 strain F and HSV-2 strain G. METHODS. Animals were treated with different amounts of AMG (0.5, O. 1, and 0.05 mg/mouse) by topical application in the eye from postinfection (PI) days -2 through +5, considering 0 the day of infection. At different PI days, development of herpetic keratitis was evaluated in treated and control mice. RESULTS. Treated animals showed a dose-dependent increase in ocular disease after viral infection, compared with control animals. Viral titers in ocular washings were higher in AMG-treated mice (PI day 2, HSV-1: AMG 0.5 mg, 1.3 × 103 plaque-forming units (PFU)/ml; control, O. 22 × 102 PFU/ml, P < 0.025). At PI day 3, control corneas had only scattered inflammatory cells, whereas those from treated animals showed a conspicuous infiltrate consisting primarily of neutrophils. Viral titers were also higher in brains of treated mice. These animals died earlier and in a greater proportion than control animals (percentage of mortality, PI day 12, HSV-1: AMG 0.5 mg, 40% ± 4%; control, 18% ± 3%, P < 0.05). CONCLUSIONS. These data indicate an inhibitory effect of nitric oxide on HSV ocular infection. |
| title |
Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice |
| title_short |
Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice |
| title_full |
Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice |
| title_fullStr |
Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice |
| title_full_unstemmed |
Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice |
| title_sort |
effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in balb/c mice |
| publishDate |
2001 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01460404_v42_n6_p1277_Benencia http://hdl.handle.net/20.500.12110/paper_01460404_v42_n6_p1277_Benencia |
| _version_ |
1768542686642438144 |