Characterization of the biosynthesis of beta(1-2) cyclic glucan in R. Fredii. Beta(1-2) glucan has no apparent role in nodule invasion of Mc Call and Peking soybean cultivars.
Three wild type strains of Rhizobium fredii, USDA 191, USDA 257 and HH 303, do not synthesize in vivo or in vitro beta(1-3), beta(1-6) cyclic glucans, all strains form in vitro and in vivo cyclic beta(1-2) glucans. Approximately 80% of the recovered R. fredii cellular cyclic beta(1-2) glucans were a...
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1996
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v42_n5_p617_InondeIannino http://hdl.handle.net/20.500.12110/paper_01455680_v42_n5_p617_InondeIannino |
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paper:paper_01455680_v42_n5_p617_InondeIannino2023-06-08T15:12:20Z Characterization of the biosynthesis of beta(1-2) cyclic glucan in R. Fredii. Beta(1-2) glucan has no apparent role in nodule invasion of Mc Call and Peking soybean cultivars. Ugalde, Rodolfo Augusto bacterial protein beta glucan cyclic beta(1 2)glucan cyclic beta(1-2)glucan glucan glycopeptide membrane protein uridine diphosphate glucose article biosynthesis chemical structure chemistry classification comparative study electron microscopy isolation and purification kinetics metabolism microbiology molecular weight Rhizobium soybean species difference symbiosis ultrastructure Bacterial Proteins beta-Glucans Glucans Glycopeptides Kinetics Membrane Proteins Microscopy, Electron Molecular Structure Molecular Weight Rhizobium Soybeans Species Specificity Symbiosis Uridine Diphosphate Glucose Three wild type strains of Rhizobium fredii, USDA 191, USDA 257 and HH 303, do not synthesize in vivo or in vitro beta(1-3), beta(1-6) cyclic glucans, all strains form in vitro and in vivo cyclic beta(1-2) glucans. Approximately 80% of the recovered R. fredii cellular cyclic beta(1-2) glucans were anionic and the substituent was identified as phosphoglycerol. Inner membranes prepared from these R. fredii strains have a beta(1-2) glucan-intermediate-protein with apparent molecular mass undistinguishable from Agrobacterium tumefaciens beta(1-2) glucan intermediate protein. Studies of the degree of polymerization of the oligosaccharides recovered from the protein-intermediate after short pulse incubations with UDP-14C-glucose suggested that the rate limiting step in the biosynthesis of cyclic glucan is cyclization. Kinetic studies revealed that the K(m) for UDP-glucose was 0.33 mM. No difference was detected between the K(m) for initiation/elongation and cyclization reactions. Nodulation studies of a ndvB R. fredii mutant with Mc Call and Peking soybean cultivars, revealed that beta(1-2) glucans do not seem to be required for normal nodule invasion of these soybean cultivars. Fil:Ugalde, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v42_n5_p617_InondeIannino http://hdl.handle.net/20.500.12110/paper_01455680_v42_n5_p617_InondeIannino |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
bacterial protein beta glucan cyclic beta(1 2)glucan cyclic beta(1-2)glucan glucan glycopeptide membrane protein uridine diphosphate glucose article biosynthesis chemical structure chemistry classification comparative study electron microscopy isolation and purification kinetics metabolism microbiology molecular weight Rhizobium soybean species difference symbiosis ultrastructure Bacterial Proteins beta-Glucans Glucans Glycopeptides Kinetics Membrane Proteins Microscopy, Electron Molecular Structure Molecular Weight Rhizobium Soybeans Species Specificity Symbiosis Uridine Diphosphate Glucose |
| spellingShingle |
bacterial protein beta glucan cyclic beta(1 2)glucan cyclic beta(1-2)glucan glucan glycopeptide membrane protein uridine diphosphate glucose article biosynthesis chemical structure chemistry classification comparative study electron microscopy isolation and purification kinetics metabolism microbiology molecular weight Rhizobium soybean species difference symbiosis ultrastructure Bacterial Proteins beta-Glucans Glucans Glycopeptides Kinetics Membrane Proteins Microscopy, Electron Molecular Structure Molecular Weight Rhizobium Soybeans Species Specificity Symbiosis Uridine Diphosphate Glucose Ugalde, Rodolfo Augusto Characterization of the biosynthesis of beta(1-2) cyclic glucan in R. Fredii. Beta(1-2) glucan has no apparent role in nodule invasion of Mc Call and Peking soybean cultivars. |
| topic_facet |
bacterial protein beta glucan cyclic beta(1 2)glucan cyclic beta(1-2)glucan glucan glycopeptide membrane protein uridine diphosphate glucose article biosynthesis chemical structure chemistry classification comparative study electron microscopy isolation and purification kinetics metabolism microbiology molecular weight Rhizobium soybean species difference symbiosis ultrastructure Bacterial Proteins beta-Glucans Glucans Glycopeptides Kinetics Membrane Proteins Microscopy, Electron Molecular Structure Molecular Weight Rhizobium Soybeans Species Specificity Symbiosis Uridine Diphosphate Glucose |
| description |
Three wild type strains of Rhizobium fredii, USDA 191, USDA 257 and HH 303, do not synthesize in vivo or in vitro beta(1-3), beta(1-6) cyclic glucans, all strains form in vitro and in vivo cyclic beta(1-2) glucans. Approximately 80% of the recovered R. fredii cellular cyclic beta(1-2) glucans were anionic and the substituent was identified as phosphoglycerol. Inner membranes prepared from these R. fredii strains have a beta(1-2) glucan-intermediate-protein with apparent molecular mass undistinguishable from Agrobacterium tumefaciens beta(1-2) glucan intermediate protein. Studies of the degree of polymerization of the oligosaccharides recovered from the protein-intermediate after short pulse incubations with UDP-14C-glucose suggested that the rate limiting step in the biosynthesis of cyclic glucan is cyclization. Kinetic studies revealed that the K(m) for UDP-glucose was 0.33 mM. No difference was detected between the K(m) for initiation/elongation and cyclization reactions. Nodulation studies of a ndvB R. fredii mutant with Mc Call and Peking soybean cultivars, revealed that beta(1-2) glucans do not seem to be required for normal nodule invasion of these soybean cultivars. |
| author |
Ugalde, Rodolfo Augusto |
| author_facet |
Ugalde, Rodolfo Augusto |
| author_sort |
Ugalde, Rodolfo Augusto |
| title |
Characterization of the biosynthesis of beta(1-2) cyclic glucan in R. Fredii. Beta(1-2) glucan has no apparent role in nodule invasion of Mc Call and Peking soybean cultivars. |
| title_short |
Characterization of the biosynthesis of beta(1-2) cyclic glucan in R. Fredii. Beta(1-2) glucan has no apparent role in nodule invasion of Mc Call and Peking soybean cultivars. |
| title_full |
Characterization of the biosynthesis of beta(1-2) cyclic glucan in R. Fredii. Beta(1-2) glucan has no apparent role in nodule invasion of Mc Call and Peking soybean cultivars. |
| title_fullStr |
Characterization of the biosynthesis of beta(1-2) cyclic glucan in R. Fredii. Beta(1-2) glucan has no apparent role in nodule invasion of Mc Call and Peking soybean cultivars. |
| title_full_unstemmed |
Characterization of the biosynthesis of beta(1-2) cyclic glucan in R. Fredii. Beta(1-2) glucan has no apparent role in nodule invasion of Mc Call and Peking soybean cultivars. |
| title_sort |
characterization of the biosynthesis of beta(1-2) cyclic glucan in r. fredii. beta(1-2) glucan has no apparent role in nodule invasion of mc call and peking soybean cultivars. |
| publishDate |
1996 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v42_n5_p617_InondeIannino http://hdl.handle.net/20.500.12110/paper_01455680_v42_n5_p617_InondeIannino |
| work_keys_str_mv |
AT ugalderodolfoaugusto characterizationofthebiosynthesisofbeta12cyclicglucaninrfrediibeta12glucanhasnoapparentroleinnoduleinvasionofmccallandpekingsoybeancultivars |
| _version_ |
1768543462544637952 |