Influence of photoinhibition on GABA and glutamic acid levels, and on glutamate decarboxylase activity in the testis and epididymis of the golden hamster
Gamma-aminobutyric acid (GABA) is found in the gonads and accessory reproductive organs, and a direct effect on steroidogenesis and sperm viability and motility has been described. The golden (Syrian) hamster is a seasonal breeder, and a pattern of regression-recrudescence in their reproductive orga...
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1996
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01056263_v19_n3_p171_Frungieri http://hdl.handle.net/20.500.12110/paper_01056263_v19_n3_p171_Frungieri |
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paper:paper_01056263_v19_n3_p171_Frungieri2023-06-08T15:10:33Z Influence of photoinhibition on GABA and glutamic acid levels, and on glutamate decarboxylase activity in the testis and epididymis of the golden hamster Epididymis GABA GAD activity Glutamic acid Golden hamster Testis 4 aminobutyric acid androstanediol androstanolone glutamate decarboxylase glutamic acid testosterone animal experiment animal tissue article controlled study enzyme activity epididymis hamster male nonhuman photoperiodicity priority journal testis testis weight Gamma-aminobutyric acid (GABA) is found in the gonads and accessory reproductive organs, and a direct effect on steroidogenesis and sperm viability and motility has been described. The golden (Syrian) hamster is a seasonal breeder, and a pattern of regression-recrudescence in their reproductive organs is observed when adult animals are exposed to less than 12.5 h daylight for an extended period of time. The purpose of this study was to investigate: (1) the presence of GABA in the testis and epididymis of golden hamsters undergoing regression and spontaneous recrudescence; (2) glutamic acid levels and glutamate decarboxylase (GAD) activity in both tissues, and (3) testicular and epididymal testosterone, dihydrotestosterone and 5α-androstane-3α, 17β-diol concentrations. Adult golden hamsters were exposed to long (LP 14L:10D) or short (SP 6L:18D) photoperiods for 9, 12, 14, 16, 18 or 22 weeks. When animals were exposed to SP for 14-16 weeks, the testis and epididymis reached maximal involution. Testicular and epididymal androgen levels showed a marked decrease (p < 0.05) during the regression period, and after 18-22 weeks, values began to recover. Between 12 and 18 weeks in SP, the testicular and epididymal content of GABA and glutamic acid was reduced significantly. The concentration of GABA in both tissues showed a sharp rise (p < 0.05), while the concentration of glutamic acid diminished during the period of maximal involution (p < 0.05). In the testis, GAD activity was increased (p < 0.001) after 14 weeks in SP, with no change in the epididymis. In conclusion, glutamic acid via GAD activity could be the main source of GABA in the testis, but not in the epididymis. Furthermore, the presence of GABA in testicular cells and its subsequent photoperiodic variations might act as an important autocrine and/or paracrine modulatory signal in gonadal processes. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01056263_v19_n3_p171_Frungieri http://hdl.handle.net/20.500.12110/paper_01056263_v19_n3_p171_Frungieri |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Epididymis GABA GAD activity Glutamic acid Golden hamster Testis 4 aminobutyric acid androstanediol androstanolone glutamate decarboxylase glutamic acid testosterone animal experiment animal tissue article controlled study enzyme activity epididymis hamster male nonhuman photoperiodicity priority journal testis testis weight |
| spellingShingle |
Epididymis GABA GAD activity Glutamic acid Golden hamster Testis 4 aminobutyric acid androstanediol androstanolone glutamate decarboxylase glutamic acid testosterone animal experiment animal tissue article controlled study enzyme activity epididymis hamster male nonhuman photoperiodicity priority journal testis testis weight Influence of photoinhibition on GABA and glutamic acid levels, and on glutamate decarboxylase activity in the testis and epididymis of the golden hamster |
| topic_facet |
Epididymis GABA GAD activity Glutamic acid Golden hamster Testis 4 aminobutyric acid androstanediol androstanolone glutamate decarboxylase glutamic acid testosterone animal experiment animal tissue article controlled study enzyme activity epididymis hamster male nonhuman photoperiodicity priority journal testis testis weight |
| description |
Gamma-aminobutyric acid (GABA) is found in the gonads and accessory reproductive organs, and a direct effect on steroidogenesis and sperm viability and motility has been described. The golden (Syrian) hamster is a seasonal breeder, and a pattern of regression-recrudescence in their reproductive organs is observed when adult animals are exposed to less than 12.5 h daylight for an extended period of time. The purpose of this study was to investigate: (1) the presence of GABA in the testis and epididymis of golden hamsters undergoing regression and spontaneous recrudescence; (2) glutamic acid levels and glutamate decarboxylase (GAD) activity in both tissues, and (3) testicular and epididymal testosterone, dihydrotestosterone and 5α-androstane-3α, 17β-diol concentrations. Adult golden hamsters were exposed to long (LP 14L:10D) or short (SP 6L:18D) photoperiods for 9, 12, 14, 16, 18 or 22 weeks. When animals were exposed to SP for 14-16 weeks, the testis and epididymis reached maximal involution. Testicular and epididymal androgen levels showed a marked decrease (p < 0.05) during the regression period, and after 18-22 weeks, values began to recover. Between 12 and 18 weeks in SP, the testicular and epididymal content of GABA and glutamic acid was reduced significantly. The concentration of GABA in both tissues showed a sharp rise (p < 0.05), while the concentration of glutamic acid diminished during the period of maximal involution (p < 0.05). In the testis, GAD activity was increased (p < 0.001) after 14 weeks in SP, with no change in the epididymis. In conclusion, glutamic acid via GAD activity could be the main source of GABA in the testis, but not in the epididymis. Furthermore, the presence of GABA in testicular cells and its subsequent photoperiodic variations might act as an important autocrine and/or paracrine modulatory signal in gonadal processes. |
| title |
Influence of photoinhibition on GABA and glutamic acid levels, and on glutamate decarboxylase activity in the testis and epididymis of the golden hamster |
| title_short |
Influence of photoinhibition on GABA and glutamic acid levels, and on glutamate decarboxylase activity in the testis and epididymis of the golden hamster |
| title_full |
Influence of photoinhibition on GABA and glutamic acid levels, and on glutamate decarboxylase activity in the testis and epididymis of the golden hamster |
| title_fullStr |
Influence of photoinhibition on GABA and glutamic acid levels, and on glutamate decarboxylase activity in the testis and epididymis of the golden hamster |
| title_full_unstemmed |
Influence of photoinhibition on GABA and glutamic acid levels, and on glutamate decarboxylase activity in the testis and epididymis of the golden hamster |
| title_sort |
influence of photoinhibition on gaba and glutamic acid levels, and on glutamate decarboxylase activity in the testis and epididymis of the golden hamster |
| publishDate |
1996 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01056263_v19_n3_p171_Frungieri http://hdl.handle.net/20.500.12110/paper_01056263_v19_n3_p171_Frungieri |
| _version_ |
1768544772460380160 |