Comparison of two coprological methods for the veterinary diagnosis of fasciolosis

The sensitivity and utility of a standard faecal sedimentation method (FSM) and a modified stool sieving staining method (FSSM), both currently employed for the diagnosis of Fasciola hepatica infection were compared. Faecal samples were obtained from 51 bovines of an endemic area for fasciolosis in...

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Autores principales: Kleiman, Florencia, Pietrokovsky, Silvia Monica, Wisnivesky Colli, María Cristina
Publicado: 2005
Materias:
Cattle
Coprological method
Diagnosis
Fasciola hepatica
Bos taurus
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01020935_v57_n2_p181_Kleiman
http://hdl.handle.net/20.500.12110/paper_01020935_v57_n2_p181_Kleiman
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_01020935_v57_n2_p181_Kleiman
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spelling paper:paper_01020935_v57_n2_p181_Kleiman2023-06-08T15:10:19Z Comparison of two coprological methods for the veterinary diagnosis of fasciolosis Kleiman, Florencia Pietrokovsky, Silvia Monica Wisnivesky Colli, María Cristina Cattle Coprological method Diagnosis Fasciola hepatica Bos taurus Fasciola hepatica The sensitivity and utility of a standard faecal sedimentation method (FSM) and a modified stool sieving staining method (FSSM), both currently employed for the diagnosis of Fasciola hepatica infection were compared. Faecal samples were obtained from 51 bovines of an endemic area for fasciolosis in Southwestern Argentina. Each sample was placed in a recipient containing 5% formalin. Eight millilitres of the suspension, equivalent to 2g of faeces, were used for each of the two methods tested. The number of eggs found per sample was recorded. The proportion of positive samples obtained by the FSSM (27/51) was significantly higher than that by the FSM (11/51) (P<0.05). The percent of agreement between methods was 41%. Over a total of 27 positive samples detected by the FSSM, the FSM missed 16, yielding 60% false negative samples. The FSSM enhanced 2.5 times the sensitivity of diagnosis. The complexity of the FSM may decrease its sensitivity through missing and loss of eggs during sample processing. These results confirmed that the commonly used FSM underestimates the prevalence and the egg output in cattle and that the FSSM is a more reliable diagnostic method. Fil:Kleiman, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pietrokovsky, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wisnivesky-Colli, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01020935_v57_n2_p181_Kleiman http://hdl.handle.net/20.500.12110/paper_01020935_v57_n2_p181_Kleiman
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Cattle
Coprological method
Diagnosis
Fasciola hepatica
Bos taurus
Fasciola hepatica
spellingShingle Cattle
Coprological method
Diagnosis
Fasciola hepatica
Bos taurus
Fasciola hepatica
Kleiman, Florencia
Pietrokovsky, Silvia Monica
Wisnivesky Colli, María Cristina
Comparison of two coprological methods for the veterinary diagnosis of fasciolosis
topic_facet Cattle
Coprological method
Diagnosis
Fasciola hepatica
Bos taurus
Fasciola hepatica
description The sensitivity and utility of a standard faecal sedimentation method (FSM) and a modified stool sieving staining method (FSSM), both currently employed for the diagnosis of Fasciola hepatica infection were compared. Faecal samples were obtained from 51 bovines of an endemic area for fasciolosis in Southwestern Argentina. Each sample was placed in a recipient containing 5% formalin. Eight millilitres of the suspension, equivalent to 2g of faeces, were used for each of the two methods tested. The number of eggs found per sample was recorded. The proportion of positive samples obtained by the FSSM (27/51) was significantly higher than that by the FSM (11/51) (P<0.05). The percent of agreement between methods was 41%. Over a total of 27 positive samples detected by the FSSM, the FSM missed 16, yielding 60% false negative samples. The FSSM enhanced 2.5 times the sensitivity of diagnosis. The complexity of the FSM may decrease its sensitivity through missing and loss of eggs during sample processing. These results confirmed that the commonly used FSM underestimates the prevalence and the egg output in cattle and that the FSSM is a more reliable diagnostic method.
author Kleiman, Florencia
Pietrokovsky, Silvia Monica
Wisnivesky Colli, María Cristina
author_facet Kleiman, Florencia
Pietrokovsky, Silvia Monica
Wisnivesky Colli, María Cristina
author_sort Kleiman, Florencia
title Comparison of two coprological methods for the veterinary diagnosis of fasciolosis
title_short Comparison of two coprological methods for the veterinary diagnosis of fasciolosis
title_full Comparison of two coprological methods for the veterinary diagnosis of fasciolosis
title_fullStr Comparison of two coprological methods for the veterinary diagnosis of fasciolosis
title_full_unstemmed Comparison of two coprological methods for the veterinary diagnosis of fasciolosis
title_sort comparison of two coprological methods for the veterinary diagnosis of fasciolosis
publishDate 2005
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01020935_v57_n2_p181_Kleiman
http://hdl.handle.net/20.500.12110/paper_01020935_v57_n2_p181_Kleiman
work_keys_str_mv AT kleimanflorencia comparisonoftwocoprologicalmethodsfortheveterinarydiagnosisoffasciolosis
AT pietrokovskysilviamonica comparisonoftwocoprologicalmethodsfortheveterinarydiagnosisoffasciolosis
AT wisniveskycollimariacristina comparisonoftwocoprologicalmethodsfortheveterinarydiagnosisoffasciolosis
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