Comparison of two coprological methods for the veterinary diagnosis of fasciolosis
The sensitivity and utility of a standard faecal sedimentation method (FSM) and a modified stool sieving staining method (FSSM), both currently employed for the diagnosis of Fasciola hepatica infection were compared. Faecal samples were obtained from 51 bovines of an endemic area for fasciolosis in...
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2005
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01020935_v57_n2_p181_Kleiman http://hdl.handle.net/20.500.12110/paper_01020935_v57_n2_p181_Kleiman |
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paper:paper_01020935_v57_n2_p181_Kleiman2023-06-08T15:10:19Z Comparison of two coprological methods for the veterinary diagnosis of fasciolosis Kleiman, Florencia Pietrokovsky, Silvia Monica Wisnivesky Colli, María Cristina Cattle Coprological method Diagnosis Fasciola hepatica Bos taurus Fasciola hepatica The sensitivity and utility of a standard faecal sedimentation method (FSM) and a modified stool sieving staining method (FSSM), both currently employed for the diagnosis of Fasciola hepatica infection were compared. Faecal samples were obtained from 51 bovines of an endemic area for fasciolosis in Southwestern Argentina. Each sample was placed in a recipient containing 5% formalin. Eight millilitres of the suspension, equivalent to 2g of faeces, were used for each of the two methods tested. The number of eggs found per sample was recorded. The proportion of positive samples obtained by the FSSM (27/51) was significantly higher than that by the FSM (11/51) (P<0.05). The percent of agreement between methods was 41%. Over a total of 27 positive samples detected by the FSSM, the FSM missed 16, yielding 60% false negative samples. The FSSM enhanced 2.5 times the sensitivity of diagnosis. The complexity of the FSM may decrease its sensitivity through missing and loss of eggs during sample processing. These results confirmed that the commonly used FSM underestimates the prevalence and the egg output in cattle and that the FSSM is a more reliable diagnostic method. Fil:Kleiman, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pietrokovsky, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wisnivesky-Colli, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01020935_v57_n2_p181_Kleiman http://hdl.handle.net/20.500.12110/paper_01020935_v57_n2_p181_Kleiman |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Cattle Coprological method Diagnosis Fasciola hepatica Bos taurus Fasciola hepatica |
spellingShingle |
Cattle Coprological method Diagnosis Fasciola hepatica Bos taurus Fasciola hepatica Kleiman, Florencia Pietrokovsky, Silvia Monica Wisnivesky Colli, María Cristina Comparison of two coprological methods for the veterinary diagnosis of fasciolosis |
topic_facet |
Cattle Coprological method Diagnosis Fasciola hepatica Bos taurus Fasciola hepatica |
description |
The sensitivity and utility of a standard faecal sedimentation method (FSM) and a modified stool sieving staining method (FSSM), both currently employed for the diagnosis of Fasciola hepatica infection were compared. Faecal samples were obtained from 51 bovines of an endemic area for fasciolosis in Southwestern Argentina. Each sample was placed in a recipient containing 5% formalin. Eight millilitres of the suspension, equivalent to 2g of faeces, were used for each of the two methods tested. The number of eggs found per sample was recorded. The proportion of positive samples obtained by the FSSM (27/51) was significantly higher than that by the FSM (11/51) (P<0.05). The percent of agreement between methods was 41%. Over a total of 27 positive samples detected by the FSSM, the FSM missed 16, yielding 60% false negative samples. The FSSM enhanced 2.5 times the sensitivity of diagnosis. The complexity of the FSM may decrease its sensitivity through missing and loss of eggs during sample processing. These results confirmed that the commonly used FSM underestimates the prevalence and the egg output in cattle and that the FSSM is a more reliable diagnostic method. |
author |
Kleiman, Florencia Pietrokovsky, Silvia Monica Wisnivesky Colli, María Cristina |
author_facet |
Kleiman, Florencia Pietrokovsky, Silvia Monica Wisnivesky Colli, María Cristina |
author_sort |
Kleiman, Florencia |
title |
Comparison of two coprological methods for the veterinary diagnosis of fasciolosis |
title_short |
Comparison of two coprological methods for the veterinary diagnosis of fasciolosis |
title_full |
Comparison of two coprological methods for the veterinary diagnosis of fasciolosis |
title_fullStr |
Comparison of two coprological methods for the veterinary diagnosis of fasciolosis |
title_full_unstemmed |
Comparison of two coprological methods for the veterinary diagnosis of fasciolosis |
title_sort |
comparison of two coprological methods for the veterinary diagnosis of fasciolosis |
publishDate |
2005 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01020935_v57_n2_p181_Kleiman http://hdl.handle.net/20.500.12110/paper_01020935_v57_n2_p181_Kleiman |
work_keys_str_mv |
AT kleimanflorencia comparisonoftwocoprologicalmethodsfortheveterinarydiagnosisoffasciolosis AT pietrokovskysilviamonica comparisonoftwocoprologicalmethodsfortheveterinarydiagnosisoffasciolosis AT wisniveskycollimariacristina comparisonoftwocoprologicalmethodsfortheveterinarydiagnosisoffasciolosis |
_version_ |
1768544588764545024 |