Chemical communication in chagas disease vectors. Source, identity, and potential function of volatiles released by the metasternal and Brindley's glands of Triatoma infestans adults

Compounds from the metasternal and Brindley's glands of the blood-sucking bug, Triatoma infestans, were identified by solid phase microextraction (SPME) and gas chromatography-mass spectrometry. Volatile compounds released by adult bugs during copulation or after mechanical disturbance were als...

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Autores principales: Manrique, Gabriel, Lazzari, Claudio Ricardo, Lorenzo, Marcelo G.
Publicado: 2006
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00980331_v32_n9_p2035_Manrique
http://hdl.handle.net/20.500.12110/paper_00980331_v32_n9_p2035_Manrique
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spelling paper:paper_00980331_v32_n9_p2035_Manrique2023-06-08T15:09:53Z Chemical communication in chagas disease vectors. Source, identity, and potential function of volatiles released by the metasternal and Brindley's glands of Triatoma infestans adults Manrique, Gabriel Lazzari, Claudio Ricardo Lorenzo, Marcelo G. Alarm pheromone Brindley's gland Chagas disease Chemical communication Male aggregation pheromone Metasternal gland SPME Triatominae insect attractants pheromone acid alcohol chemical cue copulation disease vector ester gas chromatography hemophagy insect ketone mass spectrometry physical disturbance volatile substance animal animal behavior article bioassay Chagas disease chemistry copulation disease carrier female male mass fragmentography metabolism scent gland Triatoma volatilization Animals Behavior, Animal Biological Assay Chagas Disease Copulation Female Gas Chromatography-Mass Spectrometry Insect Vectors Male Pheromones Scent Glands Triatoma Volatilization Triatoma infestans Compounds from the metasternal and Brindley's glands of the blood-sucking bug, Triatoma infestans, were identified by solid phase microextraction (SPME) and gas chromatography-mass spectrometry. Volatile compounds released by adult bugs during copulation or after mechanical disturbance were also characterized. Six compounds were identified and found consistently in all samples from metasternal glands. The most abundant were 3-pentanone, 2-methylbutanol, 3-pentanol, and an unidentified compound. The metasternal gland blends did not differ qualitatively between sexes. Compounds found in Brindley's glands were short chain acids, alcohols, esters, and a ketone with no qualitative differences between sexes. Isobutyric acid was the main component of this blend, and two new confirmed compounds were described as products of these glands: 2-butanone and 2-methylbutyric acid. 3-Pentanone was collected from the headspace over 33% of the copulating pairs of T. infestans. Volatiles found in the headspace of disturbed T. infestans adults included short-chain fatty acids, alcohols, esters, and ketones, with no qualitative differences between sexes. Both types of glands apparently discharge their contents after disturbance. However, most of the volatiles released by bugs after disturbance came from Brindley's glands. The locomotor activity of fourth instars increased significantly after stimulation with the odors emitted by disturbed adults, as compared with larvae stimulated by the odor of undisturbed adults or by clean air. We also studied the directional behavioral response of fifth instars to the disturbance scent in a locomotion compensator. Larvae exposed to volatiles released by disturbed adults walked away from the direction of the odor. The results suggest that this blend or part of it functions as an alarm pheromone for T. infestans. We suggest that the metasternal glands of this species are involved both in the sexual and the alarm contexts, and that the Brindley's glands probably have both alarm and defensive roles. © Springer Science + Business Media, Inc. 2006. Fil:Manrique, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lazzari, C.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lorenzo, M.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00980331_v32_n9_p2035_Manrique http://hdl.handle.net/20.500.12110/paper_00980331_v32_n9_p2035_Manrique
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Alarm pheromone
Brindley's gland
Chagas disease
Chemical communication
Male aggregation pheromone
Metasternal gland
SPME
Triatominae
insect attractants
pheromone
acid
alcohol
chemical cue
copulation
disease vector
ester
gas chromatography
hemophagy
insect
ketone
mass spectrometry
physical disturbance
volatile substance
animal
animal behavior
article
bioassay
Chagas disease
chemistry
copulation
disease carrier
female
male
mass fragmentography
metabolism
scent gland
Triatoma
volatilization
Animals
Behavior, Animal
Biological Assay
Chagas Disease
Copulation
Female
Gas Chromatography-Mass Spectrometry
Insect Vectors
Male
Pheromones
Scent Glands
Triatoma
Volatilization
Triatoma infestans
spellingShingle Alarm pheromone
Brindley's gland
Chagas disease
Chemical communication
Male aggregation pheromone
Metasternal gland
SPME
Triatominae
insect attractants
pheromone
acid
alcohol
chemical cue
copulation
disease vector
ester
gas chromatography
hemophagy
insect
ketone
mass spectrometry
physical disturbance
volatile substance
animal
animal behavior
article
bioassay
Chagas disease
chemistry
copulation
disease carrier
female
male
mass fragmentography
metabolism
scent gland
Triatoma
volatilization
Animals
Behavior, Animal
Biological Assay
Chagas Disease
Copulation
Female
Gas Chromatography-Mass Spectrometry
Insect Vectors
Male
Pheromones
Scent Glands
Triatoma
Volatilization
Triatoma infestans
Manrique, Gabriel
Lazzari, Claudio Ricardo
Lorenzo, Marcelo G.
Chemical communication in chagas disease vectors. Source, identity, and potential function of volatiles released by the metasternal and Brindley's glands of Triatoma infestans adults
topic_facet Alarm pheromone
Brindley's gland
Chagas disease
Chemical communication
Male aggregation pheromone
Metasternal gland
SPME
Triatominae
insect attractants
pheromone
acid
alcohol
chemical cue
copulation
disease vector
ester
gas chromatography
hemophagy
insect
ketone
mass spectrometry
physical disturbance
volatile substance
animal
animal behavior
article
bioassay
Chagas disease
chemistry
copulation
disease carrier
female
male
mass fragmentography
metabolism
scent gland
Triatoma
volatilization
Animals
Behavior, Animal
Biological Assay
Chagas Disease
Copulation
Female
Gas Chromatography-Mass Spectrometry
Insect Vectors
Male
Pheromones
Scent Glands
Triatoma
Volatilization
Triatoma infestans
description Compounds from the metasternal and Brindley's glands of the blood-sucking bug, Triatoma infestans, were identified by solid phase microextraction (SPME) and gas chromatography-mass spectrometry. Volatile compounds released by adult bugs during copulation or after mechanical disturbance were also characterized. Six compounds were identified and found consistently in all samples from metasternal glands. The most abundant were 3-pentanone, 2-methylbutanol, 3-pentanol, and an unidentified compound. The metasternal gland blends did not differ qualitatively between sexes. Compounds found in Brindley's glands were short chain acids, alcohols, esters, and a ketone with no qualitative differences between sexes. Isobutyric acid was the main component of this blend, and two new confirmed compounds were described as products of these glands: 2-butanone and 2-methylbutyric acid. 3-Pentanone was collected from the headspace over 33% of the copulating pairs of T. infestans. Volatiles found in the headspace of disturbed T. infestans adults included short-chain fatty acids, alcohols, esters, and ketones, with no qualitative differences between sexes. Both types of glands apparently discharge their contents after disturbance. However, most of the volatiles released by bugs after disturbance came from Brindley's glands. The locomotor activity of fourth instars increased significantly after stimulation with the odors emitted by disturbed adults, as compared with larvae stimulated by the odor of undisturbed adults or by clean air. We also studied the directional behavioral response of fifth instars to the disturbance scent in a locomotion compensator. Larvae exposed to volatiles released by disturbed adults walked away from the direction of the odor. The results suggest that this blend or part of it functions as an alarm pheromone for T. infestans. We suggest that the metasternal glands of this species are involved both in the sexual and the alarm contexts, and that the Brindley's glands probably have both alarm and defensive roles. © Springer Science + Business Media, Inc. 2006.
author Manrique, Gabriel
Lazzari, Claudio Ricardo
Lorenzo, Marcelo G.
author_facet Manrique, Gabriel
Lazzari, Claudio Ricardo
Lorenzo, Marcelo G.
author_sort Manrique, Gabriel
title Chemical communication in chagas disease vectors. Source, identity, and potential function of volatiles released by the metasternal and Brindley's glands of Triatoma infestans adults
title_short Chemical communication in chagas disease vectors. Source, identity, and potential function of volatiles released by the metasternal and Brindley's glands of Triatoma infestans adults
title_full Chemical communication in chagas disease vectors. Source, identity, and potential function of volatiles released by the metasternal and Brindley's glands of Triatoma infestans adults
title_fullStr Chemical communication in chagas disease vectors. Source, identity, and potential function of volatiles released by the metasternal and Brindley's glands of Triatoma infestans adults
title_full_unstemmed Chemical communication in chagas disease vectors. Source, identity, and potential function of volatiles released by the metasternal and Brindley's glands of Triatoma infestans adults
title_sort chemical communication in chagas disease vectors. source, identity, and potential function of volatiles released by the metasternal and brindley's glands of triatoma infestans adults
publishDate 2006
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00980331_v32_n9_p2035_Manrique
http://hdl.handle.net/20.500.12110/paper_00980331_v32_n9_p2035_Manrique
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