An alternate solution of fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 2. Diffusion of fluorescein in aqueous sucrose solutions

The traditional analysis of the fluorescence recovery kinetics after spot bleaching yields expressions for the diffusion coefficient of the probe that are not suitable for linear fittings. In a previous work we developed an improved recovery function that is a better alternative for data analysis. T...

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Autores principales: Corti, Horacio Roberto, Frank, Guillermo A., Marconi, Mario Carlos
Publicado: 2008
Materias:
Diffusion
Fluorescein
Fluorescence recovery
Photobleaching
Stokes-Einstein equation
Sucrose solutions
Supercooled
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00959782_v37_n11_p1593_Corti
http://hdl.handle.net/20.500.12110/paper_00959782_v37_n11_p1593_Corti
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00959782_v37_n11_p1593_Corti
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spelling paper:paper_00959782_v37_n11_p1593_Corti2023-06-08T15:09:45Z An alternate solution of fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 2. Diffusion of fluorescein in aqueous sucrose solutions Corti, Horacio Roberto Frank, Guillermo A. Marconi, Mario Carlos Diffusion Fluorescein Fluorescence recovery Photobleaching Stokes-Einstein equation Sucrose solutions Supercooled The traditional analysis of the fluorescence recovery kinetics after spot bleaching yields expressions for the diffusion coefficient of the probe that are not suitable for linear fittings. In a previous work we developed an improved recovery function that is a better alternative for data analysis. To illustrate its application to real cases and compare it with the previous data treatment, we measured the time response of fluorescein in aqueous sucrose solutions, covering the unsaturated and the supercooled region, where decoupling between diffusion and viscosity is observed. The results are compared with the mobility of different types of solutes in aqueous sucrose solutions and are discussed in terms of the classical hydrodynamic model. © 2008 Springer Science+Business Media, LLC. Fil:Corti, H.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Frank, G.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Marconi, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00959782_v37_n11_p1593_Corti http://hdl.handle.net/20.500.12110/paper_00959782_v37_n11_p1593_Corti
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Diffusion
Fluorescein
Fluorescence recovery
Photobleaching
Stokes-Einstein equation
Sucrose solutions
Supercooled
spellingShingle Diffusion
Fluorescein
Fluorescence recovery
Photobleaching
Stokes-Einstein equation
Sucrose solutions
Supercooled
Corti, Horacio Roberto
Frank, Guillermo A.
Marconi, Mario Carlos
An alternate solution of fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 2. Diffusion of fluorescein in aqueous sucrose solutions
topic_facet Diffusion
Fluorescein
Fluorescence recovery
Photobleaching
Stokes-Einstein equation
Sucrose solutions
Supercooled
description The traditional analysis of the fluorescence recovery kinetics after spot bleaching yields expressions for the diffusion coefficient of the probe that are not suitable for linear fittings. In a previous work we developed an improved recovery function that is a better alternative for data analysis. To illustrate its application to real cases and compare it with the previous data treatment, we measured the time response of fluorescein in aqueous sucrose solutions, covering the unsaturated and the supercooled region, where decoupling between diffusion and viscosity is observed. The results are compared with the mobility of different types of solutes in aqueous sucrose solutions and are discussed in terms of the classical hydrodynamic model. © 2008 Springer Science+Business Media, LLC.
author Corti, Horacio Roberto
Frank, Guillermo A.
Marconi, Mario Carlos
author_facet Corti, Horacio Roberto
Frank, Guillermo A.
Marconi, Mario Carlos
author_sort Corti, Horacio Roberto
title An alternate solution of fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 2. Diffusion of fluorescein in aqueous sucrose solutions
title_short An alternate solution of fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 2. Diffusion of fluorescein in aqueous sucrose solutions
title_full An alternate solution of fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 2. Diffusion of fluorescein in aqueous sucrose solutions
title_fullStr An alternate solution of fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 2. Diffusion of fluorescein in aqueous sucrose solutions
title_full_unstemmed An alternate solution of fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 2. Diffusion of fluorescein in aqueous sucrose solutions
title_sort alternate solution of fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 2. diffusion of fluorescein in aqueous sucrose solutions
publishDate 2008
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00959782_v37_n11_p1593_Corti
http://hdl.handle.net/20.500.12110/paper_00959782_v37_n11_p1593_Corti
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