Cycloheximide inhibits context memory and long-term habituation in the crab Chasmagnathus
A shadow moving over head elicits an escape response in the crab Chasmagnathus that habituates promptly and for a long period. The effect of the protein synthesis inhibitor Cycloheximide (CY) on this long-term memory was analyzed. Two hours after injection, 10 μg CY inhibited [14C]-amino acid incorp...
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1995
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00913057_v52_n2_p385_Pedreira http://hdl.handle.net/20.500.12110/paper_00913057_v52_n2_p385_Pedreira |
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paper:paper_00913057_v52_n2_p385_Pedreira2023-06-08T15:08:03Z Cycloheximide inhibits context memory and long-term habituation in the crab Chasmagnathus Pedreira, Maria Eugenia Dimant, Beatriz Tomsic, Daniel Quesada Allué, Luis Alberto Arthropoda Crab Cycloheximide Habituation Memory amino acid mixture cycloheximide protein synthesis inhibitor radioisotope amnesia animal experiment animal tissue article brain controlled study crab escape behavior ganglion habituation long term memory male nonhuman priority journal protein synthesis short term memory training Amino Acids Amnesia Animal Brachyura Conditioning, Operant Cycloheximide Escape Reaction Ganglia, Invertebrate Habituation (Psychophysiology) Male Memory Protein Synthesis Inhibitors Support, Non-U.S. Gov't Animalia Arthropoda Chasmagnathus Decapoda (Crustacea) A shadow moving over head elicits an escape response in the crab Chasmagnathus that habituates promptly and for a long period. The effect of the protein synthesis inhibitor Cycloheximide (CY) on this long-term memory was analyzed. Two hours after injection, 10 μg CY inhibited [14C]-amino acid incorporation into cerebral plus thoracic ganglia by 88% and 20 μg by 92%, but no inhibition was found at 24 h. A single injection of 10-20 μg, CY given 30 min before training, failed to affect the short-term habituation. Similar doses impaired both context memory (CM) and long-term habituation (LTH) when tested at 72 and 120 h but only CM at 24 h. Such a disparity was explained by an unspecific depressing effect upon the response, attributed to an interaction between CY and training. The hypothesis was confirmed, because CY injected immediately after training disclosed amnestic effect at 24 h on both CM and LTH. A similar effect was proven when animals were injected at 2 h but not at 6 h after training. Results from experiments with pretraining and pretesting injections put aside a state-dependence or retrieval deficit effects of the drug. Taken together, findings of this article argue strongly for de novo protein synthesis as a mechanism of LTH and for the close relation between CM and LTH. © 1995. Fil:Pedreira, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dimant, B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tomsic, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Quesada-Allue, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1995 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00913057_v52_n2_p385_Pedreira http://hdl.handle.net/20.500.12110/paper_00913057_v52_n2_p385_Pedreira |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arthropoda Crab Cycloheximide Habituation Memory amino acid mixture cycloheximide protein synthesis inhibitor radioisotope amnesia animal experiment animal tissue article brain controlled study crab escape behavior ganglion habituation long term memory male nonhuman priority journal protein synthesis short term memory training Amino Acids Amnesia Animal Brachyura Conditioning, Operant Cycloheximide Escape Reaction Ganglia, Invertebrate Habituation (Psychophysiology) Male Memory Protein Synthesis Inhibitors Support, Non-U.S. Gov't Animalia Arthropoda Chasmagnathus Decapoda (Crustacea) |
| spellingShingle |
Arthropoda Crab Cycloheximide Habituation Memory amino acid mixture cycloheximide protein synthesis inhibitor radioisotope amnesia animal experiment animal tissue article brain controlled study crab escape behavior ganglion habituation long term memory male nonhuman priority journal protein synthesis short term memory training Amino Acids Amnesia Animal Brachyura Conditioning, Operant Cycloheximide Escape Reaction Ganglia, Invertebrate Habituation (Psychophysiology) Male Memory Protein Synthesis Inhibitors Support, Non-U.S. Gov't Animalia Arthropoda Chasmagnathus Decapoda (Crustacea) Pedreira, Maria Eugenia Dimant, Beatriz Tomsic, Daniel Quesada Allué, Luis Alberto Cycloheximide inhibits context memory and long-term habituation in the crab Chasmagnathus |
| topic_facet |
Arthropoda Crab Cycloheximide Habituation Memory amino acid mixture cycloheximide protein synthesis inhibitor radioisotope amnesia animal experiment animal tissue article brain controlled study crab escape behavior ganglion habituation long term memory male nonhuman priority journal protein synthesis short term memory training Amino Acids Amnesia Animal Brachyura Conditioning, Operant Cycloheximide Escape Reaction Ganglia, Invertebrate Habituation (Psychophysiology) Male Memory Protein Synthesis Inhibitors Support, Non-U.S. Gov't Animalia Arthropoda Chasmagnathus Decapoda (Crustacea) |
| description |
A shadow moving over head elicits an escape response in the crab Chasmagnathus that habituates promptly and for a long period. The effect of the protein synthesis inhibitor Cycloheximide (CY) on this long-term memory was analyzed. Two hours after injection, 10 μg CY inhibited [14C]-amino acid incorporation into cerebral plus thoracic ganglia by 88% and 20 μg by 92%, but no inhibition was found at 24 h. A single injection of 10-20 μg, CY given 30 min before training, failed to affect the short-term habituation. Similar doses impaired both context memory (CM) and long-term habituation (LTH) when tested at 72 and 120 h but only CM at 24 h. Such a disparity was explained by an unspecific depressing effect upon the response, attributed to an interaction between CY and training. The hypothesis was confirmed, because CY injected immediately after training disclosed amnestic effect at 24 h on both CM and LTH. A similar effect was proven when animals were injected at 2 h but not at 6 h after training. Results from experiments with pretraining and pretesting injections put aside a state-dependence or retrieval deficit effects of the drug. Taken together, findings of this article argue strongly for de novo protein synthesis as a mechanism of LTH and for the close relation between CM and LTH. © 1995. |
| author |
Pedreira, Maria Eugenia Dimant, Beatriz Tomsic, Daniel Quesada Allué, Luis Alberto |
| author_facet |
Pedreira, Maria Eugenia Dimant, Beatriz Tomsic, Daniel Quesada Allué, Luis Alberto |
| author_sort |
Pedreira, Maria Eugenia |
| title |
Cycloheximide inhibits context memory and long-term habituation in the crab Chasmagnathus |
| title_short |
Cycloheximide inhibits context memory and long-term habituation in the crab Chasmagnathus |
| title_full |
Cycloheximide inhibits context memory and long-term habituation in the crab Chasmagnathus |
| title_fullStr |
Cycloheximide inhibits context memory and long-term habituation in the crab Chasmagnathus |
| title_full_unstemmed |
Cycloheximide inhibits context memory and long-term habituation in the crab Chasmagnathus |
| title_sort |
cycloheximide inhibits context memory and long-term habituation in the crab chasmagnathus |
| publishDate |
1995 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00913057_v52_n2_p385_Pedreira http://hdl.handle.net/20.500.12110/paper_00913057_v52_n2_p385_Pedreira |
| work_keys_str_mv |
AT pedreiramariaeugenia cycloheximideinhibitscontextmemoryandlongtermhabituationinthecrabchasmagnathus AT dimantbeatriz cycloheximideinhibitscontextmemoryandlongtermhabituationinthecrabchasmagnathus AT tomsicdaniel cycloheximideinhibitscontextmemoryandlongtermhabituationinthecrabchasmagnathus AT quesadaallueluisalberto cycloheximideinhibitscontextmemoryandlongtermhabituationinthecrabchasmagnathus |
| _version_ |
1768546293492219904 |