Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge

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A molecular approach was used to evaluate the effect of nonylphenol ethoxylate surfactants on the bacterial diversity in lab-scale activated sludge reactors. Separate bench-scale units were fed synthetic wastewater with and without addition of branched nonylphenol ethoxylates (NPnEO). The performanc...

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Autores principales:	Itria, Raúl Fabio, Figuerola, Eva Lucía Margarita, Babay, Paola Alejandra, Erijman, Leonardo
Publicado:	2004
Materias:	Activated sludge Betaproteobacteria Community structure Fluorescence in situ hybridization Nonionic surfactant Nonylphenol ethoxylates Bacteria Carbonaceous adsorbents Composition Degradation Probes Removal Solutions Surface active agents Wastewater Bacterial community Bench-scale units Feeding solutions Sludge reactors Sewage sludge 4',6 diamidino 2 phenylindole nonylphenol nonylphenol ethoxylate ribosome RNA surfactant unclassified drug activated sludge bacterium wastewater treatment article bacterial flora bacterial strain bacterium identification controlled study dot hybridization evaluation Gram negative bacterium in situ hybridization microbial diversity nonhuman oligonucleotide probe priority journal reactor separation technique sludge waste water management Aerobiosis Animals Base Sequence Biodegradation, Environmental Ethylene Glycols Fishes Fluorescent Dyes Gammaproteobacteria In Situ Hybridization Oligonucleotide Probes RNA, Ribosomal, 16S Sewage Soil Microbiology Surface-Active Agents Time Factors Bacteria (microorganisms) Proteobacteria
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00431354_v38_n8_p2077_Lozada http://hdl.handle.net/20.500.12110/paper_00431354_v38_n8_p2077_Lozada
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00431354_v38_n8_p2077_Lozada
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spelling	paper:paper_00431354_v38_n8_p2077_Lozada2023-06-08T15:04:55Z Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge Itria, Raúl Fabio Figuerola, Eva Lucía Margarita Babay, Paola Alejandra Erijman, Leonardo Activated sludge Betaproteobacteria Community structure Fluorescence in situ hybridization Nonionic surfactant Nonylphenol ethoxylates Bacteria Carbonaceous adsorbents Composition Degradation Probes Removal Solutions Surface active agents Wastewater Bacterial community Bench-scale units Feeding solutions Sludge reactors Sewage sludge 4',6 diamidino 2 phenylindole nonylphenol nonylphenol ethoxylate ribosome RNA surfactant unclassified drug activated sludge bacterium surfactant wastewater treatment article bacterial flora bacterial strain bacterium identification controlled study dot hybridization evaluation Gram negative bacterium in situ hybridization microbial diversity nonhuman oligonucleotide probe priority journal reactor separation technique sludge waste water management Aerobiosis Animals Bacteria Base Sequence Betaproteobacteria Biodegradation, Environmental Ethylene Glycols Fishes Fluorescent Dyes Gammaproteobacteria In Situ Hybridization Oligonucleotide Probes RNA, Ribosomal, 16S Sewage Soil Microbiology Surface-Active Agents Time Factors Bacteria (microorganisms) Betaproteobacteria Proteobacteria A molecular approach was used to evaluate the effect of nonylphenol ethoxylate surfactants on the bacterial diversity in lab-scale activated sludge reactors. Separate bench-scale units were fed synthetic wastewater with and without addition of branched nonylphenol ethoxylates (NPnEO). The performance of the reactors, in terms of carbonaceous removal was largely unaffected by the presence of NP10EO in the feeding solution. However, addition of NP10EO exerted a pronounced shift in bacterial community composition. In situ hybridization analyzing larger phylogenetic groups of bacteria with ribosomal RNA-targeted oligonucleotide probes revealed the dominance of clusters composed of Betaproteobacteria, accounting for up to one-third of 4′,6-diamidino-2- phenylindol-dihydrochloride (DAPI)-stained cells in NP10EO amended reactors and only 5% of DAPI-stained cells in the controls. These shifts in populations of larger phylogenetic groups were confirmed by dot-blot analysis of rRNA. Members of gamma subclass of Proteobacteria were present in low numbers in all activated sludge samples examined, suggesting that only bacteria affiliated with the beta subclass of Proteobacteria may have a specific role in NP10EO degradation. © 2004 Elsevier Ltd. All rights reserved. Fil:Itria, R.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Figuerola, E.L.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Babay, P.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Erijman, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00431354_v38_n8_p2077_Lozada http://hdl.handle.net/20.500.12110/paper_00431354_v38_n8_p2077_Lozada
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Activated sludge Betaproteobacteria Community structure Fluorescence in situ hybridization Nonionic surfactant Nonylphenol ethoxylates Bacteria Carbonaceous adsorbents Composition Degradation Probes Removal Solutions Surface active agents Wastewater Bacterial community Bench-scale units Feeding solutions Sludge reactors Sewage sludge 4',6 diamidino 2 phenylindole nonylphenol nonylphenol ethoxylate ribosome RNA surfactant unclassified drug activated sludge bacterium surfactant wastewater treatment article bacterial flora bacterial strain bacterium identification controlled study dot hybridization evaluation Gram negative bacterium in situ hybridization microbial diversity nonhuman oligonucleotide probe priority journal reactor separation technique sludge waste water management Aerobiosis Animals Bacteria Base Sequence Betaproteobacteria Biodegradation, Environmental Ethylene Glycols Fishes Fluorescent Dyes Gammaproteobacteria In Situ Hybridization Oligonucleotide Probes RNA, Ribosomal, 16S Sewage Soil Microbiology Surface-Active Agents Time Factors Bacteria (microorganisms) Betaproteobacteria Proteobacteria
spellingShingle	Activated sludge Betaproteobacteria Community structure Fluorescence in situ hybridization Nonionic surfactant Nonylphenol ethoxylates Bacteria Carbonaceous adsorbents Composition Degradation Probes Removal Solutions Surface active agents Wastewater Bacterial community Bench-scale units Feeding solutions Sludge reactors Sewage sludge 4',6 diamidino 2 phenylindole nonylphenol nonylphenol ethoxylate ribosome RNA surfactant unclassified drug activated sludge bacterium surfactant wastewater treatment article bacterial flora bacterial strain bacterium identification controlled study dot hybridization evaluation Gram negative bacterium in situ hybridization microbial diversity nonhuman oligonucleotide probe priority journal reactor separation technique sludge waste water management Aerobiosis Animals Bacteria Base Sequence Betaproteobacteria Biodegradation, Environmental Ethylene Glycols Fishes Fluorescent Dyes Gammaproteobacteria In Situ Hybridization Oligonucleotide Probes RNA, Ribosomal, 16S Sewage Soil Microbiology Surface-Active Agents Time Factors Bacteria (microorganisms) Betaproteobacteria Proteobacteria Itria, Raúl Fabio Figuerola, Eva Lucía Margarita Babay, Paola Alejandra Erijman, Leonardo Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge
topic_facet	Activated sludge Betaproteobacteria Community structure Fluorescence in situ hybridization Nonionic surfactant Nonylphenol ethoxylates Bacteria Carbonaceous adsorbents Composition Degradation Probes Removal Solutions Surface active agents Wastewater Bacterial community Bench-scale units Feeding solutions Sludge reactors Sewage sludge 4',6 diamidino 2 phenylindole nonylphenol nonylphenol ethoxylate ribosome RNA surfactant unclassified drug activated sludge bacterium surfactant wastewater treatment article bacterial flora bacterial strain bacterium identification controlled study dot hybridization evaluation Gram negative bacterium in situ hybridization microbial diversity nonhuman oligonucleotide probe priority journal reactor separation technique sludge waste water management Aerobiosis Animals Bacteria Base Sequence Betaproteobacteria Biodegradation, Environmental Ethylene Glycols Fishes Fluorescent Dyes Gammaproteobacteria In Situ Hybridization Oligonucleotide Probes RNA, Ribosomal, 16S Sewage Soil Microbiology Surface-Active Agents Time Factors Bacteria (microorganisms) Betaproteobacteria Proteobacteria
description	A molecular approach was used to evaluate the effect of nonylphenol ethoxylate surfactants on the bacterial diversity in lab-scale activated sludge reactors. Separate bench-scale units were fed synthetic wastewater with and without addition of branched nonylphenol ethoxylates (NPnEO). The performance of the reactors, in terms of carbonaceous removal was largely unaffected by the presence of NP10EO in the feeding solution. However, addition of NP10EO exerted a pronounced shift in bacterial community composition. In situ hybridization analyzing larger phylogenetic groups of bacteria with ribosomal RNA-targeted oligonucleotide probes revealed the dominance of clusters composed of Betaproteobacteria, accounting for up to one-third of 4′,6-diamidino-2- phenylindol-dihydrochloride (DAPI)-stained cells in NP10EO amended reactors and only 5% of DAPI-stained cells in the controls. These shifts in populations of larger phylogenetic groups were confirmed by dot-blot analysis of rRNA. Members of gamma subclass of Proteobacteria were present in low numbers in all activated sludge samples examined, suggesting that only bacteria affiliated with the beta subclass of Proteobacteria may have a specific role in NP10EO degradation. © 2004 Elsevier Ltd. All rights reserved.
author	Itria, Raúl Fabio Figuerola, Eva Lucía Margarita Babay, Paola Alejandra Erijman, Leonardo
author_facet	Itria, Raúl Fabio Figuerola, Eva Lucía Margarita Babay, Paola Alejandra Erijman, Leonardo
author_sort	Itria, Raúl Fabio
title	Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge
title_short	Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge
title_full	Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge
title_fullStr	Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge
title_full_unstemmed	Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge
title_sort	bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge
publishDate	2004
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00431354_v38_n8_p2077_Lozada http://hdl.handle.net/20.500.12110/paper_00431354_v38_n8_p2077_Lozada
work_keys_str_mv	AT itriaraulfabio bacterialcommunityshiftsinnonylphenolpolyethoxylatesenrichedactivatedsludge AT figuerolaevaluciamargarita bacterialcommunityshiftsinnonylphenolpolyethoxylatesenrichedactivatedsludge AT babaypaolaalejandra bacterialcommunityshiftsinnonylphenolpolyethoxylatesenrichedactivatedsludge AT erijmanleonardo bacterialcommunityshiftsinnonylphenolpolyethoxylatesenrichedactivatedsludge
_version_	1768541930703028224

Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge

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