Human hepatocytes apoptosis induced by replication of hepatitis B virus subgenotypes F1b and F4: Role of basal core promoter and preCore mutations
In the context of pathogenesis of HBV infection, HBV genotypes and mutants have been shown to affect the natural course of chronic infection and treatment outcomes. In this work, we studied the induction of apoptosis by the replication of HBV subgenotypes F1b and F4, and the naturally occurring muta...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00426822_v513_n_p160_Elizalde http://hdl.handle.net/20.500.12110/paper_00426822_v513_n_p160_Elizalde |
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paper:paper_00426822_v513_n_p160_Elizalde2023-06-08T15:04:52Z Human hepatocytes apoptosis induced by replication of hepatitis B virus subgenotypes F1b and F4: Role of basal core promoter and preCore mutations Apoptosis Basal core promoter mutations Hepatitis B virus Pathogenesis PreCore mutation Subgenotype F1b Subgenotype F4 caspase 3 hepatitis B(e) antigen protein Bax protein bcl 2 protein bcl x hepatitis B core antigen antigen expression apoptosis Article basal core gene cell adhesion cell contact cell viability controlled study down regulation enzyme activity gene expression Hepatitis B virus Hepatitis B virus subtype F1b Hepatitis B virus subtype F4 human human cell liver cell mitochondrion nonhuman preCore gene priority journal promoter region protein expression virus gene virus replication cell line genetics Hepatitis B virus liver cell mutation physiology promoter region virology Apoptosis Cell Line Hepatitis B Core Antigens Hepatitis B virus Hepatocytes Humans Mutation Promoter Regions, Genetic Virus Replication In the context of pathogenesis of HBV infection, HBV genotypes and mutants have been shown to affect the natural course of chronic infection and treatment outcomes. In this work, we studied the induction of apoptosis by the replication of HBV subgenotypes F1b and F4, and the naturally occurring mutants BCP and preCore. Both subgenotypes F1b and F4 HBV genome transfections induced cell death by apoptosis in human hepatocytes. The BCPdm (A1762T/G1764A) and preCore (G1896A) mutants induced higher levels of apoptosis than the wt virus. This increase in apoptosis was not associated with the enhanced viral replication of the variants. HBV-mediated apoptosis was independent of viral subgenotypes, and associated with the modulation of members of the regulatory Bcl-2 family proteins expression in the mitochondrial apoptotic pathway. Finally, the apoptosis induction increase observed for the preCore mutants suggests that HBeAg might have an anti-apoptotic effect in human hepatocytes. © 2017 Elsevier Inc. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00426822_v513_n_p160_Elizalde http://hdl.handle.net/20.500.12110/paper_00426822_v513_n_p160_Elizalde |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Apoptosis Basal core promoter mutations Hepatitis B virus Pathogenesis PreCore mutation Subgenotype F1b Subgenotype F4 caspase 3 hepatitis B(e) antigen protein Bax protein bcl 2 protein bcl x hepatitis B core antigen antigen expression apoptosis Article basal core gene cell adhesion cell contact cell viability controlled study down regulation enzyme activity gene expression Hepatitis B virus Hepatitis B virus subtype F1b Hepatitis B virus subtype F4 human human cell liver cell mitochondrion nonhuman preCore gene priority journal promoter region protein expression virus gene virus replication cell line genetics Hepatitis B virus liver cell mutation physiology promoter region virology Apoptosis Cell Line Hepatitis B Core Antigens Hepatitis B virus Hepatocytes Humans Mutation Promoter Regions, Genetic Virus Replication |
| spellingShingle |
Apoptosis Basal core promoter mutations Hepatitis B virus Pathogenesis PreCore mutation Subgenotype F1b Subgenotype F4 caspase 3 hepatitis B(e) antigen protein Bax protein bcl 2 protein bcl x hepatitis B core antigen antigen expression apoptosis Article basal core gene cell adhesion cell contact cell viability controlled study down regulation enzyme activity gene expression Hepatitis B virus Hepatitis B virus subtype F1b Hepatitis B virus subtype F4 human human cell liver cell mitochondrion nonhuman preCore gene priority journal promoter region protein expression virus gene virus replication cell line genetics Hepatitis B virus liver cell mutation physiology promoter region virology Apoptosis Cell Line Hepatitis B Core Antigens Hepatitis B virus Hepatocytes Humans Mutation Promoter Regions, Genetic Virus Replication Human hepatocytes apoptosis induced by replication of hepatitis B virus subgenotypes F1b and F4: Role of basal core promoter and preCore mutations |
| topic_facet |
Apoptosis Basal core promoter mutations Hepatitis B virus Pathogenesis PreCore mutation Subgenotype F1b Subgenotype F4 caspase 3 hepatitis B(e) antigen protein Bax protein bcl 2 protein bcl x hepatitis B core antigen antigen expression apoptosis Article basal core gene cell adhesion cell contact cell viability controlled study down regulation enzyme activity gene expression Hepatitis B virus Hepatitis B virus subtype F1b Hepatitis B virus subtype F4 human human cell liver cell mitochondrion nonhuman preCore gene priority journal promoter region protein expression virus gene virus replication cell line genetics Hepatitis B virus liver cell mutation physiology promoter region virology Apoptosis Cell Line Hepatitis B Core Antigens Hepatitis B virus Hepatocytes Humans Mutation Promoter Regions, Genetic Virus Replication |
| description |
In the context of pathogenesis of HBV infection, HBV genotypes and mutants have been shown to affect the natural course of chronic infection and treatment outcomes. In this work, we studied the induction of apoptosis by the replication of HBV subgenotypes F1b and F4, and the naturally occurring mutants BCP and preCore. Both subgenotypes F1b and F4 HBV genome transfections induced cell death by apoptosis in human hepatocytes. The BCPdm (A1762T/G1764A) and preCore (G1896A) mutants induced higher levels of apoptosis than the wt virus. This increase in apoptosis was not associated with the enhanced viral replication of the variants. HBV-mediated apoptosis was independent of viral subgenotypes, and associated with the modulation of members of the regulatory Bcl-2 family proteins expression in the mitochondrial apoptotic pathway. Finally, the apoptosis induction increase observed for the preCore mutants suggests that HBeAg might have an anti-apoptotic effect in human hepatocytes. © 2017 Elsevier Inc. |
| title |
Human hepatocytes apoptosis induced by replication of hepatitis B virus subgenotypes F1b and F4: Role of basal core promoter and preCore mutations |
| title_short |
Human hepatocytes apoptosis induced by replication of hepatitis B virus subgenotypes F1b and F4: Role of basal core promoter and preCore mutations |
| title_full |
Human hepatocytes apoptosis induced by replication of hepatitis B virus subgenotypes F1b and F4: Role of basal core promoter and preCore mutations |
| title_fullStr |
Human hepatocytes apoptosis induced by replication of hepatitis B virus subgenotypes F1b and F4: Role of basal core promoter and preCore mutations |
| title_full_unstemmed |
Human hepatocytes apoptosis induced by replication of hepatitis B virus subgenotypes F1b and F4: Role of basal core promoter and preCore mutations |
| title_sort |
human hepatocytes apoptosis induced by replication of hepatitis b virus subgenotypes f1b and f4: role of basal core promoter and precore mutations |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00426822_v513_n_p160_Elizalde http://hdl.handle.net/20.500.12110/paper_00426822_v513_n_p160_Elizalde |
| _version_ |
1768542403599269888 |