Biosynthesis of bufadienolides in toads. V. the origin of the cholesterol used by toad parotoid glands for biosynthesis of bufadienolides
Sodium (1- 14 C) acetate and (5- 3 H) mevalonate were incubated with Bufo vienarum toad parotoid gland and liver tissues. Both labelled compounds were incorporated into cholesterol produced by liver while the incubations with parotoid gland produced no labelled cholesterol. Low and high density lipo...
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1984
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0039128X_v44_n1_p11_SantaColoma http://hdl.handle.net/20.500.12110/paper_0039128X_v44_n1_p11_SantaColoma |
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paper:paper_0039128X_v44_n1_p11_SantaColoma2023-06-08T15:03:13Z Biosynthesis of bufadienolides in toads. V. the origin of the cholesterol used by toad parotoid glands for biosynthesis of bufadienolides Santa Coloma, Tomás Antonio Garraffo, Hugo Martín Pignataro, Omar Pedro Charreau, Eduardo Hernán Gros, Eduardo Gervasio bufadienolide cholesterol animal cell mouth nonhuman parotid gland priority journal Animals Binding Sites Biological Transport, Active Bufanolides Bufo arenarum Cholesterol Cholesterol Esters Female Lipoproteins, HDL Lipoproteins, LDL Liver Parotid Gland Animalia Anura Bufo arenarum Sodium (1- 14 C) acetate and (5- 3 H) mevalonate were incubated with Bufo vienarum toad parotoid gland and liver tissues. Both labelled compounds were incorporated into cholesterol produced by liver while the incubations with parotoid gland produced no labelled cholesterol. Low and high density lipoproteins isolated from toad plasma were iodinated and used for binding studies. Membrane preparations of paratoid gland showed high affinity binding sites for 125 I-LDL and 125 I-HDL. In addition, while colchicine inhibits the in vitro uptake of ( 3 H) cholesteryl linoleate-LDL into parotoid gland tissue an opposite effect was seen with ( 3 H) cholesteryl linoleate-HDL. The above mentioned results would support the hypothesis that the cholesterol used by the parotoid gland for the biosynthesis of bufalienolides would be produced in the liver, transported by the circulating lipoproteins and incorporated by the glands by a receptor-mediated mechanism. © 1984. Fil:Santa Coloma, ?.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Garraffo, H.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pignataro, O.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gros, E.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1984 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0039128X_v44_n1_p11_SantaColoma http://hdl.handle.net/20.500.12110/paper_0039128X_v44_n1_p11_SantaColoma |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
bufadienolide cholesterol animal cell mouth nonhuman parotid gland priority journal Animals Binding Sites Biological Transport, Active Bufanolides Bufo arenarum Cholesterol Cholesterol Esters Female Lipoproteins, HDL Lipoproteins, LDL Liver Parotid Gland Animalia Anura Bufo arenarum |
spellingShingle |
bufadienolide cholesterol animal cell mouth nonhuman parotid gland priority journal Animals Binding Sites Biological Transport, Active Bufanolides Bufo arenarum Cholesterol Cholesterol Esters Female Lipoproteins, HDL Lipoproteins, LDL Liver Parotid Gland Animalia Anura Bufo arenarum Santa Coloma, Tomás Antonio Garraffo, Hugo Martín Pignataro, Omar Pedro Charreau, Eduardo Hernán Gros, Eduardo Gervasio Biosynthesis of bufadienolides in toads. V. the origin of the cholesterol used by toad parotoid glands for biosynthesis of bufadienolides |
topic_facet |
bufadienolide cholesterol animal cell mouth nonhuman parotid gland priority journal Animals Binding Sites Biological Transport, Active Bufanolides Bufo arenarum Cholesterol Cholesterol Esters Female Lipoproteins, HDL Lipoproteins, LDL Liver Parotid Gland Animalia Anura Bufo arenarum |
description |
Sodium (1- 14 C) acetate and (5- 3 H) mevalonate were incubated with Bufo vienarum toad parotoid gland and liver tissues. Both labelled compounds were incorporated into cholesterol produced by liver while the incubations with parotoid gland produced no labelled cholesterol. Low and high density lipoproteins isolated from toad plasma were iodinated and used for binding studies. Membrane preparations of paratoid gland showed high affinity binding sites for 125 I-LDL and 125 I-HDL. In addition, while colchicine inhibits the in vitro uptake of ( 3 H) cholesteryl linoleate-LDL into parotoid gland tissue an opposite effect was seen with ( 3 H) cholesteryl linoleate-HDL. The above mentioned results would support the hypothesis that the cholesterol used by the parotoid gland for the biosynthesis of bufalienolides would be produced in the liver, transported by the circulating lipoproteins and incorporated by the glands by a receptor-mediated mechanism. © 1984. |
author |
Santa Coloma, Tomás Antonio Garraffo, Hugo Martín Pignataro, Omar Pedro Charreau, Eduardo Hernán Gros, Eduardo Gervasio |
author_facet |
Santa Coloma, Tomás Antonio Garraffo, Hugo Martín Pignataro, Omar Pedro Charreau, Eduardo Hernán Gros, Eduardo Gervasio |
author_sort |
Santa Coloma, Tomás Antonio |
title |
Biosynthesis of bufadienolides in toads. V. the origin of the cholesterol used by toad parotoid glands for biosynthesis of bufadienolides |
title_short |
Biosynthesis of bufadienolides in toads. V. the origin of the cholesterol used by toad parotoid glands for biosynthesis of bufadienolides |
title_full |
Biosynthesis of bufadienolides in toads. V. the origin of the cholesterol used by toad parotoid glands for biosynthesis of bufadienolides |
title_fullStr |
Biosynthesis of bufadienolides in toads. V. the origin of the cholesterol used by toad parotoid glands for biosynthesis of bufadienolides |
title_full_unstemmed |
Biosynthesis of bufadienolides in toads. V. the origin of the cholesterol used by toad parotoid glands for biosynthesis of bufadienolides |
title_sort |
biosynthesis of bufadienolides in toads. v. the origin of the cholesterol used by toad parotoid glands for biosynthesis of bufadienolides |
publishDate |
1984 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0039128X_v44_n1_p11_SantaColoma http://hdl.handle.net/20.500.12110/paper_0039128X_v44_n1_p11_SantaColoma |
work_keys_str_mv |
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1768542026337353728 |