Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)
Caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis, has a high prevalence in many regions of the world, including Argentina and Brazil. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for the identification of this microorganism was designed ba...
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00345288_v92_n2_p202_Pavan http://hdl.handle.net/20.500.12110/paper_00345288_v92_n2_p202_Pavan |
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paper:paper_00345288_v92_n2_p202_Pavan2023-06-08T15:00:43Z Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB) Pettinari, María Julia Caseous lymphadenitis Corynebacterium pseudotuberculosis RpoB PCR-RFLP endonuclease protein MseI protein StuI unclassified drug analytic method Arcanobacterium pyogenes article bacterial gene bacterium identification bacterium isolation computer analysis controlled study Corynebacterium pseudotuberculosis Corynebacterium ulcerans molecular typing nonhuman nucleotide sequence polymerase chain reaction reliability restriction fragment length polymorphism RNA polymerase beta subunit gene rpoB gene sequence analysis sheep species differentiation unindexed sequence wild type Animals Corynebacterium Infections Corynebacterium pseudotuberculosis DNA-Directed RNA Polymerases Polymorphism, Restriction Fragment Length Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis Sheep Sheep Diseases Animalia Arcanobacterium pyogenes Capra Corynebacterium Corynebacterium pseudotuberculosis Ovis Ovis aries Caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis, has a high prevalence in many regions of the world, including Argentina and Brazil. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for the identification of this microorganism was designed based on the hypervariable region of the polymorphic RNA polymerase β-subunit gene (rpoB). All available Corynebacterium rpoB sequences were analyzed by computer-assisted restriction analysis. The rpoB PCR-RFLP pattern predicted by using endonucleases MseI and StuI clearly differentiated C. pseudotuberculosis from sixty-one other Corynebacterium species. This method was successfully applied to identify twelve wild C. pseudotuberculosis ovine isolates and one caprine isolate. It was also used to differentiate C. pseudotuberculosis from Arcanobacterium pyogenes, an ovine pathogen with similar clinical characteristics. These results indicate that this new molecular method can be used for the reliable identification of the pathogen, essential for the timely detection of infected animals and for epidemiological studies. © 2011 Elsevier Ltd. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00345288_v92_n2_p202_Pavan http://hdl.handle.net/20.500.12110/paper_00345288_v92_n2_p202_Pavan |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Caseous lymphadenitis Corynebacterium pseudotuberculosis RpoB PCR-RFLP endonuclease protein MseI protein StuI unclassified drug analytic method Arcanobacterium pyogenes article bacterial gene bacterium identification bacterium isolation computer analysis controlled study Corynebacterium pseudotuberculosis Corynebacterium ulcerans molecular typing nonhuman nucleotide sequence polymerase chain reaction reliability restriction fragment length polymorphism RNA polymerase beta subunit gene rpoB gene sequence analysis sheep species differentiation unindexed sequence wild type Animals Corynebacterium Infections Corynebacterium pseudotuberculosis DNA-Directed RNA Polymerases Polymorphism, Restriction Fragment Length Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis Sheep Sheep Diseases Animalia Arcanobacterium pyogenes Capra Corynebacterium Corynebacterium pseudotuberculosis Ovis Ovis aries |
spellingShingle |
Caseous lymphadenitis Corynebacterium pseudotuberculosis RpoB PCR-RFLP endonuclease protein MseI protein StuI unclassified drug analytic method Arcanobacterium pyogenes article bacterial gene bacterium identification bacterium isolation computer analysis controlled study Corynebacterium pseudotuberculosis Corynebacterium ulcerans molecular typing nonhuman nucleotide sequence polymerase chain reaction reliability restriction fragment length polymorphism RNA polymerase beta subunit gene rpoB gene sequence analysis sheep species differentiation unindexed sequence wild type Animals Corynebacterium Infections Corynebacterium pseudotuberculosis DNA-Directed RNA Polymerases Polymorphism, Restriction Fragment Length Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis Sheep Sheep Diseases Animalia Arcanobacterium pyogenes Capra Corynebacterium Corynebacterium pseudotuberculosis Ovis Ovis aries Pettinari, María Julia Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB) |
topic_facet |
Caseous lymphadenitis Corynebacterium pseudotuberculosis RpoB PCR-RFLP endonuclease protein MseI protein StuI unclassified drug analytic method Arcanobacterium pyogenes article bacterial gene bacterium identification bacterium isolation computer analysis controlled study Corynebacterium pseudotuberculosis Corynebacterium ulcerans molecular typing nonhuman nucleotide sequence polymerase chain reaction reliability restriction fragment length polymorphism RNA polymerase beta subunit gene rpoB gene sequence analysis sheep species differentiation unindexed sequence wild type Animals Corynebacterium Infections Corynebacterium pseudotuberculosis DNA-Directed RNA Polymerases Polymorphism, Restriction Fragment Length Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis Sheep Sheep Diseases Animalia Arcanobacterium pyogenes Capra Corynebacterium Corynebacterium pseudotuberculosis Ovis Ovis aries |
description |
Caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis, has a high prevalence in many regions of the world, including Argentina and Brazil. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for the identification of this microorganism was designed based on the hypervariable region of the polymorphic RNA polymerase β-subunit gene (rpoB). All available Corynebacterium rpoB sequences were analyzed by computer-assisted restriction analysis. The rpoB PCR-RFLP pattern predicted by using endonucleases MseI and StuI clearly differentiated C. pseudotuberculosis from sixty-one other Corynebacterium species. This method was successfully applied to identify twelve wild C. pseudotuberculosis ovine isolates and one caprine isolate. It was also used to differentiate C. pseudotuberculosis from Arcanobacterium pyogenes, an ovine pathogen with similar clinical characteristics. These results indicate that this new molecular method can be used for the reliable identification of the pathogen, essential for the timely detection of infected animals and for epidemiological studies. © 2011 Elsevier Ltd. |
author |
Pettinari, María Julia |
author_facet |
Pettinari, María Julia |
author_sort |
Pettinari, María Julia |
title |
Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB) |
title_short |
Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB) |
title_full |
Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB) |
title_fullStr |
Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB) |
title_full_unstemmed |
Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB) |
title_sort |
identification of corynebacterium pseudotuberculosis from sheep by pcr-restriction analysis using the rna polymerase β-subunit gene (rpob) |
publishDate |
2012 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00345288_v92_n2_p202_Pavan http://hdl.handle.net/20.500.12110/paper_00345288_v92_n2_p202_Pavan |
work_keys_str_mv |
AT pettinarimariajulia identificationofcorynebacteriumpseudotuberculosisfromsheepbypcrrestrictionanalysisusingthernapolymerasebsubunitgenerpob |
_version_ |
1768546106781728768 |