Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation
This study evaluated sexual dimorphism and seasonal variations in corticotrophs and adrenal zona fasciculata in dogs, as well as the expression of oestrogen receptor alpha (ERα). An immunohistochemical analysis was conducted in pituitaries for ACTH and in adrenal glands for ERα and for the melanocor...
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2016
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00345288_v104_n_p10_Gallelli http://hdl.handle.net/20.500.12110/paper_00345288_v104_n_p10_Gallelli |
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paper:paper_00345288_v104_n_p10_Gallelli2023-06-08T15:00:41Z Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation Vissio, Paula Gabriela Adrenal cortex Corticotroph Melanocortin 2 receptor (MC2R) Oestrogen receptor Sexual dimorphism corticotropin estrogen receptor alpha melanocortin 2 receptor estrogen receptor alpha ACTH secreting cell animal tissue Article controlled study dog female immunohistochemistry male nonhuman optical density protein analysis protein expression seasonal variation sex difference summer winter zona fasciculata anatomy and histology animal chemistry genetics hypophysis adrenal system hypothalamus hypophysis system metabolism physiology season sexual development veterinary Animals Dogs Estrogen Receptor alpha Female Hypothalamo-Hypophyseal System Immunohistochemistry Male Pituitary-Adrenal System Seasons Sex Characteristics This study evaluated sexual dimorphism and seasonal variations in corticotrophs and adrenal zona fasciculata in dogs, as well as the expression of oestrogen receptor alpha (ERα). An immunohistochemical analysis was conducted in pituitaries for ACTH and in adrenal glands for ERα and for the melanocortin-2-receptor (MC2R) in winter and summer. Double immunofluorescence was performed to identify ERα in corticotrophs. Females had a greater proportion of corticotrophs per field (p < 0.01), with a greater cellular area and optical density (p < 0.001) than males. Optical density of corticotrophs was greater in winter for both sexes (p < 0.001). In zona fasciculata, ERα and MC2R expression was greater in females (p < 0.001) and was greater in winter (p < 0.001). ERα was identified in corticotrophs. This study is the first to demonstrate ERα expression in corticotrophs and the adrenal cortex in dogs, providing evidence for sexual dimorphism and seasonal variations. © 2015 Elsevier Ltd. Fil:Vissio, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00345288_v104_n_p10_Gallelli http://hdl.handle.net/20.500.12110/paper_00345288_v104_n_p10_Gallelli |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Adrenal cortex Corticotroph Melanocortin 2 receptor (MC2R) Oestrogen receptor Sexual dimorphism corticotropin estrogen receptor alpha melanocortin 2 receptor estrogen receptor alpha ACTH secreting cell animal tissue Article controlled study dog female immunohistochemistry male nonhuman optical density protein analysis protein expression seasonal variation sex difference summer winter zona fasciculata anatomy and histology animal chemistry genetics hypophysis adrenal system hypothalamus hypophysis system metabolism physiology season sexual development veterinary Animals Dogs Estrogen Receptor alpha Female Hypothalamo-Hypophyseal System Immunohistochemistry Male Pituitary-Adrenal System Seasons Sex Characteristics |
| spellingShingle |
Adrenal cortex Corticotroph Melanocortin 2 receptor (MC2R) Oestrogen receptor Sexual dimorphism corticotropin estrogen receptor alpha melanocortin 2 receptor estrogen receptor alpha ACTH secreting cell animal tissue Article controlled study dog female immunohistochemistry male nonhuman optical density protein analysis protein expression seasonal variation sex difference summer winter zona fasciculata anatomy and histology animal chemistry genetics hypophysis adrenal system hypothalamus hypophysis system metabolism physiology season sexual development veterinary Animals Dogs Estrogen Receptor alpha Female Hypothalamo-Hypophyseal System Immunohistochemistry Male Pituitary-Adrenal System Seasons Sex Characteristics Vissio, Paula Gabriela Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation |
| topic_facet |
Adrenal cortex Corticotroph Melanocortin 2 receptor (MC2R) Oestrogen receptor Sexual dimorphism corticotropin estrogen receptor alpha melanocortin 2 receptor estrogen receptor alpha ACTH secreting cell animal tissue Article controlled study dog female immunohistochemistry male nonhuman optical density protein analysis protein expression seasonal variation sex difference summer winter zona fasciculata anatomy and histology animal chemistry genetics hypophysis adrenal system hypothalamus hypophysis system metabolism physiology season sexual development veterinary Animals Dogs Estrogen Receptor alpha Female Hypothalamo-Hypophyseal System Immunohistochemistry Male Pituitary-Adrenal System Seasons Sex Characteristics |
| description |
This study evaluated sexual dimorphism and seasonal variations in corticotrophs and adrenal zona fasciculata in dogs, as well as the expression of oestrogen receptor alpha (ERα). An immunohistochemical analysis was conducted in pituitaries for ACTH and in adrenal glands for ERα and for the melanocortin-2-receptor (MC2R) in winter and summer. Double immunofluorescence was performed to identify ERα in corticotrophs. Females had a greater proportion of corticotrophs per field (p < 0.01), with a greater cellular area and optical density (p < 0.001) than males. Optical density of corticotrophs was greater in winter for both sexes (p < 0.001). In zona fasciculata, ERα and MC2R expression was greater in females (p < 0.001) and was greater in winter (p < 0.001). ERα was identified in corticotrophs. This study is the first to demonstrate ERα expression in corticotrophs and the adrenal cortex in dogs, providing evidence for sexual dimorphism and seasonal variations. © 2015 Elsevier Ltd. |
| author |
Vissio, Paula Gabriela |
| author_facet |
Vissio, Paula Gabriela |
| author_sort |
Vissio, Paula Gabriela |
| title |
Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation |
| title_short |
Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation |
| title_full |
Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation |
| title_fullStr |
Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation |
| title_full_unstemmed |
Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation |
| title_sort |
immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: sex-linked and seasonal variation |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00345288_v104_n_p10_Gallelli http://hdl.handle.net/20.500.12110/paper_00345288_v104_n_p10_Gallelli |
| work_keys_str_mv |
AT vissiopaulagabriela immunohistochemicalanalysisofthehypothalamicpituitaryadrenalaxisindogssexlinkedandseasonalvariation |
| _version_ |
1768544901454102528 |