RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii

The tripeptide Arg-Gly-Asp (RGD) (1 mM) as well as the polymer ProNectin F (20 nM) added to culture medium of the fungus Mucor rouxii (defined medium) produced a delay in the switch from isodiametric growth to tip growth; at the time of germination the mother cell had a 4.6 times larger volume with...

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Autores principales:	Pereyra, Elba Nora, Argimón, Silvia, Moreno de Colonna, Silvia
Publicado:	2003
Materias:	Arg-Gly-Asp tripeptide Cell morphology Cyclic AMP Mucor rouxii ProNectin F Actins Cell Size Cytochalasins Fibronectins Mucor Oligopeptides Recombinant Proteins Signal Transduction Spores, Fungal Amylomyces rouxii Fungi
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0033183X_v222_n1-2_p23_Pereyra http://hdl.handle.net/20.500.12110/paper_0033183X_v222_n1-2_p23_Pereyra
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0033183X_v222_n1-2_p23_Pereyra
record_format	dspace
spelling	paper:paper_0033183X_v222_n1-2_p23_Pereyra2023-06-08T15:00:23Z RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii Pereyra, Elba Nora Argimón, Silvia Moreno de Colonna, Silvia Arg-Gly-Asp tripeptide Cell morphology Cyclic AMP Mucor rouxii ProNectin F Actins Cell Size Cyclic AMP Cytochalasins Fibronectins Mucor Oligopeptides Recombinant Proteins Signal Transduction Spores, Fungal Amylomyces rouxii Fungi Mucor The tripeptide Arg-Gly-Asp (RGD) (1 mM) as well as the polymer ProNectin F (20 nM) added to culture medium of the fungus Mucor rouxii (defined medium) produced a delay in the switch from isodiametric growth to tip growth; at the time of germination the mother cell had a 4.6 times larger volume with 3.6 times more germ tubes per cell than control germinating sporangiospores. Disruption of the actin network with 2 μg of cytochalasin A per ml blocked the switch to tip growth; the effect was analogous to the one of 150 μM dibutyryl-cyclic AMP (cAMP), which we previously described to promote isodiametric growth via protein kinase A. 150 μM dibutyryl-cAMP antagonises partially the effect of 1 mM RGD; the cells still emit several germ tubes per mother cell but their number is smaller and the volume of the cell at germ tube emission is larger than with RGD alone. At higher concentrations the dibutyryl-cAMP overrides completely the effect of RGD. Our results suggest that M. rouxii has an RGD recognition system and demonstrate that RGD-containing peptides have a profound effect on the isotropic stage of growth and on the establishment of cell polarity and that cAMP analogues can override this effect. Fil:Pereyra, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Argimón, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Moreno, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0033183X_v222_n1-2_p23_Pereyra http://hdl.handle.net/20.500.12110/paper_0033183X_v222_n1-2_p23_Pereyra
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Arg-Gly-Asp tripeptide Cell morphology Cyclic AMP Mucor rouxii ProNectin F Actins Cell Size Cyclic AMP Cytochalasins Fibronectins Mucor Oligopeptides Recombinant Proteins Signal Transduction Spores, Fungal Amylomyces rouxii Fungi Mucor
spellingShingle	Arg-Gly-Asp tripeptide Cell morphology Cyclic AMP Mucor rouxii ProNectin F Actins Cell Size Cyclic AMP Cytochalasins Fibronectins Mucor Oligopeptides Recombinant Proteins Signal Transduction Spores, Fungal Amylomyces rouxii Fungi Mucor Pereyra, Elba Nora Argimón, Silvia Moreno de Colonna, Silvia RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii
topic_facet	Arg-Gly-Asp tripeptide Cell morphology Cyclic AMP Mucor rouxii ProNectin F Actins Cell Size Cyclic AMP Cytochalasins Fibronectins Mucor Oligopeptides Recombinant Proteins Signal Transduction Spores, Fungal Amylomyces rouxii Fungi Mucor
description	The tripeptide Arg-Gly-Asp (RGD) (1 mM) as well as the polymer ProNectin F (20 nM) added to culture medium of the fungus Mucor rouxii (defined medium) produced a delay in the switch from isodiametric growth to tip growth; at the time of germination the mother cell had a 4.6 times larger volume with 3.6 times more germ tubes per cell than control germinating sporangiospores. Disruption of the actin network with 2 μg of cytochalasin A per ml blocked the switch to tip growth; the effect was analogous to the one of 150 μM dibutyryl-cyclic AMP (cAMP), which we previously described to promote isodiametric growth via protein kinase A. 150 μM dibutyryl-cAMP antagonises partially the effect of 1 mM RGD; the cells still emit several germ tubes per mother cell but their number is smaller and the volume of the cell at germ tube emission is larger than with RGD alone. At higher concentrations the dibutyryl-cAMP overrides completely the effect of RGD. Our results suggest that M. rouxii has an RGD recognition system and demonstrate that RGD-containing peptides have a profound effect on the isotropic stage of growth and on the establishment of cell polarity and that cAMP analogues can override this effect.
author	Pereyra, Elba Nora Argimón, Silvia Moreno de Colonna, Silvia
author_facet	Pereyra, Elba Nora Argimón, Silvia Moreno de Colonna, Silvia
author_sort	Pereyra, Elba Nora
title	RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii
title_short	RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii
title_full	RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii
title_fullStr	RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii
title_full_unstemmed	RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii
title_sort	rgd-containing peptides and cyclic amp have antagonistic roles in the morphology of mucor rouxii
publishDate	2003
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0033183X_v222_n1-2_p23_Pereyra http://hdl.handle.net/20.500.12110/paper_0033183X_v222_n1-2_p23_Pereyra
work_keys_str_mv	AT pereyraelbanora rgdcontainingpeptidesandcyclicamphaveantagonisticrolesinthemorphologyofmucorrouxii AT argimonsilvia rgdcontainingpeptidesandcyclicamphaveantagonisticrolesinthemorphologyofmucorrouxii AT morenodecolonnasilvia rgdcontainingpeptidesandcyclicamphaveantagonisticrolesinthemorphologyofmucorrouxii
_version_	1768546434663055360

RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii

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