Antiviral activity of a carrageenan from Gigartina skottsbergii against intraperitoneal murine herpes simplex virus infection
The partially cyclized μ/v-carrageenan 1C3, isolated from the red seaweed Gigartina skottsbergii, was previously shown to be a potent inhibitor of the in vitro replication of Herpes simplex virus types 1 (HSV-1) and 2 (HSV-2). Here the protective effect of 1C3 in a murine model of intraperitoneal (i...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Publicado: |
2006
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320943_v72_n2_p121_Pujol http://hdl.handle.net/20.500.12110/paper_00320943_v72_n2_p121_Pujol |
| Aporte de: |
| id |
paper:paper_00320943_v72_n2_p121_Pujol |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_00320943_v72_n2_p121_Pujol2023-06-08T15:00:06Z Antiviral activity of a carrageenan from Gigartina skottsbergii against intraperitoneal murine herpes simplex virus infection Pujol, Carlos Alberto Scolaro, Luis Alberto Ciancia, Marina Matulewicz, María Cristina Cerezo, Alberto Saúl Damonte, Elsa Beatriz Antiviral agent Gigartina skottsbergii Gigartinaceae Gigartinales Herpes simplex virus Mouse intraperitoneal infection Natural carrageenan Pharmacokinetics carrageenan Gigartina skottsbergii extract plant extract unclassified drug animal model antiviral activity article body weight controlled study dose response drug bioavailability drug blood level drug half life drug mechanism herpes simplex Herpes simplex virus 1 Herpes simplex virus 2 mouse nonhuman survival Algae, Red Animals Antiviral Agents Carrageenan Disease Models, Animal Herpes Simplex Injections, Intraperitoneal Male Mice Phytotherapy Plant Preparations Tissue Distribution algae Animalia Gigartina skottsbergii Gigartinaceae Gigartinales Herpes Human herpesvirus 1 Human herpesvirus 2 Murinae Simplexvirus The partially cyclized μ/v-carrageenan 1C3, isolated from the red seaweed Gigartina skottsbergii, was previously shown to be a potent inhibitor of the in vitro replication of Herpes simplex virus types 1 (HSV-1) and 2 (HSV-2). Here the protective effect of 1C3 in a murine model of intraperitoneal (i.p.) HSV-1 infection was evaluated. OF1 mice were i.p. infected with 5 × 105 PFU of HSV-1 KOS strain, and the effects of different treatments with 1C3 were studied. When 30 mg/kg of body weight of 1C3 was administered by the i.p. route immediately after HSV-1 infection, 87.5% survival of the animals was achieved (p < 0.005), associated with a delay in the mean day of death in 1C3-treated non-surviving mice. Animal survival was not improved when multiple doses of 1C3 were also given in the period 1-48 h post-infection, and no protection was afforded when treatment was started after 24 h of infection. When virus and compound were injected by different routes, i.p. and intravenous (i.v.), respectively, a still significant protection was achieved (40% survival, p < 0.05). No toxicity of 1C3 for the animals was recorded. The pharmacokinetic properties were analyzed after injection of 1C3 into the tail vein by monitoring of [3H]-1C3 in plasma and organs and by a bioassay of the anti-HSV-1 activity remaining in serum after non-radioactive 1C3 inoculation. A very rapid disappearance of the compound from the blood was observed since only 5.9-0.9% of the radioactivity of the initially administered [3H]-1C3 appeared in the plasma between 5-300 minutes after administration. A transient peak of radioactivity was detected in the kidney 15 minutes after inoculation. The bioassay confirms the presence of the compound circulating in a biologically active form up to 1 hour after injection. © Georg Thieme Verlag KG Stuttgart. Fil:Pujol, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Scolaro, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ciancia, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matulewicz, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerezo, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320943_v72_n2_p121_Pujol http://hdl.handle.net/20.500.12110/paper_00320943_v72_n2_p121_Pujol |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antiviral agent Gigartina skottsbergii Gigartinaceae Gigartinales Herpes simplex virus Mouse intraperitoneal infection Natural carrageenan Pharmacokinetics carrageenan Gigartina skottsbergii extract plant extract unclassified drug animal model antiviral activity article body weight controlled study dose response drug bioavailability drug blood level drug half life drug mechanism herpes simplex Herpes simplex virus 1 Herpes simplex virus 2 mouse nonhuman survival Algae, Red Animals Antiviral Agents Carrageenan Disease Models, Animal Herpes Simplex Injections, Intraperitoneal Male Mice Phytotherapy Plant Preparations Tissue Distribution algae Animalia Gigartina skottsbergii Gigartinaceae Gigartinales Herpes Human herpesvirus 1 Human herpesvirus 2 Murinae Simplexvirus |
| spellingShingle |
Antiviral agent Gigartina skottsbergii Gigartinaceae Gigartinales Herpes simplex virus Mouse intraperitoneal infection Natural carrageenan Pharmacokinetics carrageenan Gigartina skottsbergii extract plant extract unclassified drug animal model antiviral activity article body weight controlled study dose response drug bioavailability drug blood level drug half life drug mechanism herpes simplex Herpes simplex virus 1 Herpes simplex virus 2 mouse nonhuman survival Algae, Red Animals Antiviral Agents Carrageenan Disease Models, Animal Herpes Simplex Injections, Intraperitoneal Male Mice Phytotherapy Plant Preparations Tissue Distribution algae Animalia Gigartina skottsbergii Gigartinaceae Gigartinales Herpes Human herpesvirus 1 Human herpesvirus 2 Murinae Simplexvirus Pujol, Carlos Alberto Scolaro, Luis Alberto Ciancia, Marina Matulewicz, María Cristina Cerezo, Alberto Saúl Damonte, Elsa Beatriz Antiviral activity of a carrageenan from Gigartina skottsbergii against intraperitoneal murine herpes simplex virus infection |
| topic_facet |
Antiviral agent Gigartina skottsbergii Gigartinaceae Gigartinales Herpes simplex virus Mouse intraperitoneal infection Natural carrageenan Pharmacokinetics carrageenan Gigartina skottsbergii extract plant extract unclassified drug animal model antiviral activity article body weight controlled study dose response drug bioavailability drug blood level drug half life drug mechanism herpes simplex Herpes simplex virus 1 Herpes simplex virus 2 mouse nonhuman survival Algae, Red Animals Antiviral Agents Carrageenan Disease Models, Animal Herpes Simplex Injections, Intraperitoneal Male Mice Phytotherapy Plant Preparations Tissue Distribution algae Animalia Gigartina skottsbergii Gigartinaceae Gigartinales Herpes Human herpesvirus 1 Human herpesvirus 2 Murinae Simplexvirus |
| description |
The partially cyclized μ/v-carrageenan 1C3, isolated from the red seaweed Gigartina skottsbergii, was previously shown to be a potent inhibitor of the in vitro replication of Herpes simplex virus types 1 (HSV-1) and 2 (HSV-2). Here the protective effect of 1C3 in a murine model of intraperitoneal (i.p.) HSV-1 infection was evaluated. OF1 mice were i.p. infected with 5 × 105 PFU of HSV-1 KOS strain, and the effects of different treatments with 1C3 were studied. When 30 mg/kg of body weight of 1C3 was administered by the i.p. route immediately after HSV-1 infection, 87.5% survival of the animals was achieved (p < 0.005), associated with a delay in the mean day of death in 1C3-treated non-surviving mice. Animal survival was not improved when multiple doses of 1C3 were also given in the period 1-48 h post-infection, and no protection was afforded when treatment was started after 24 h of infection. When virus and compound were injected by different routes, i.p. and intravenous (i.v.), respectively, a still significant protection was achieved (40% survival, p < 0.05). No toxicity of 1C3 for the animals was recorded. The pharmacokinetic properties were analyzed after injection of 1C3 into the tail vein by monitoring of [3H]-1C3 in plasma and organs and by a bioassay of the anti-HSV-1 activity remaining in serum after non-radioactive 1C3 inoculation. A very rapid disappearance of the compound from the blood was observed since only 5.9-0.9% of the radioactivity of the initially administered [3H]-1C3 appeared in the plasma between 5-300 minutes after administration. A transient peak of radioactivity was detected in the kidney 15 minutes after inoculation. The bioassay confirms the presence of the compound circulating in a biologically active form up to 1 hour after injection. © Georg Thieme Verlag KG Stuttgart. |
| author |
Pujol, Carlos Alberto Scolaro, Luis Alberto Ciancia, Marina Matulewicz, María Cristina Cerezo, Alberto Saúl Damonte, Elsa Beatriz |
| author_facet |
Pujol, Carlos Alberto Scolaro, Luis Alberto Ciancia, Marina Matulewicz, María Cristina Cerezo, Alberto Saúl Damonte, Elsa Beatriz |
| author_sort |
Pujol, Carlos Alberto |
| title |
Antiviral activity of a carrageenan from Gigartina skottsbergii against intraperitoneal murine herpes simplex virus infection |
| title_short |
Antiviral activity of a carrageenan from Gigartina skottsbergii against intraperitoneal murine herpes simplex virus infection |
| title_full |
Antiviral activity of a carrageenan from Gigartina skottsbergii against intraperitoneal murine herpes simplex virus infection |
| title_fullStr |
Antiviral activity of a carrageenan from Gigartina skottsbergii against intraperitoneal murine herpes simplex virus infection |
| title_full_unstemmed |
Antiviral activity of a carrageenan from Gigartina skottsbergii against intraperitoneal murine herpes simplex virus infection |
| title_sort |
antiviral activity of a carrageenan from gigartina skottsbergii against intraperitoneal murine herpes simplex virus infection |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320943_v72_n2_p121_Pujol http://hdl.handle.net/20.500.12110/paper_00320943_v72_n2_p121_Pujol |
| work_keys_str_mv |
AT pujolcarlosalberto antiviralactivityofacarrageenanfromgigartinaskottsbergiiagainstintraperitonealmurineherpessimplexvirusinfection AT scolaroluisalberto antiviralactivityofacarrageenanfromgigartinaskottsbergiiagainstintraperitonealmurineherpessimplexvirusinfection AT cianciamarina antiviralactivityofacarrageenanfromgigartinaskottsbergiiagainstintraperitonealmurineherpessimplexvirusinfection AT matulewiczmariacristina antiviralactivityofacarrageenanfromgigartinaskottsbergiiagainstintraperitonealmurineherpessimplexvirusinfection AT cerezoalbertosaul antiviralactivityofacarrageenanfromgigartinaskottsbergiiagainstintraperitonealmurineherpessimplexvirusinfection AT damonteelsabeatriz antiviralactivityofacarrageenanfromgigartinaskottsbergiiagainstintraperitonealmurineherpessimplexvirusinfection |
| _version_ |
1768544541514661888 |