The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth

In flowering plants, the process of pollen germination and tube growth is required for successful fertilization. A pollen receptor kinase from tomato (Solanum lycopersicum), LePRK2, has been implicated in signaling during pollen germination and tube growth as well as in mediating pollen (tube)-pisti...

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Autores principales:	Wengier, Diego Leonardo, Muschietti, Jorge P.
Publicado:	2008
Materias:	hybrid protein primer DNA protein kinase article enzymology germination growth, development and aging metabolism molecular genetics nucleotide sequence physiology pollen polymerase chain reaction signal transduction tomato transgenic plant Base Sequence DNA Primers Germination Lycopersicon esculentum Molecular Sequence Data Plants, Genetically Modified Pollen Polymerase Chain Reaction Protein Kinases Recombinant Fusion Proteins Signal Transduction Magnoliophyta Solanum
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320889_v148_n3_p1368_Zhang http://hdl.handle.net/20.500.12110/paper_00320889_v148_n3_p1368_Zhang
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00320889_v148_n3_p1368_Zhang
record_format	dspace
spelling	paper:paper_00320889_v148_n3_p1368_Zhang2023-06-08T14:59:59Z The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth Wengier, Diego Leonardo Muschietti, Jorge P. hybrid protein primer DNA protein kinase article enzymology germination growth, development and aging metabolism molecular genetics nucleotide sequence physiology pollen polymerase chain reaction signal transduction tomato transgenic plant Base Sequence DNA Primers Germination Lycopersicon esculentum Molecular Sequence Data Plants, Genetically Modified Pollen Polymerase Chain Reaction Protein Kinases Recombinant Fusion Proteins Signal Transduction Lycopersicon esculentum Magnoliophyta Solanum In flowering plants, the process of pollen germination and tube growth is required for successful fertilization. A pollen receptor kinase from tomato (Solanum lycopersicum), LePRK2, has been implicated in signaling during pollen germination and tube growth as well as in mediating pollen (tube)-pistil communication. Here we show that reduced expression of LePRK2 affects four aspects of pollen germination and tube growth. First, the percentage of pollen that germinates is reduced, and the time window for competence to germinate is also shorter. Second, the pollen tube growth rate is reduced both in vitro and in the pistil. Third, tip-localized superoxide production by pollen tubes cannot be increased by exogenous calcium ions. Fourth, pollen tubes have defects in responses to style extract component (STIL), an extracellular growth-promoting signal from the pistil. Pollen tubes transiently overexpressing LePRK2-fluorescent protein fusions had slightly wider tips, whereas pollen tubes coexpressing LePRK2 and its cytoplasmic partner protein KPP (a Rop-GEF) had much wider tips. Together these results show that LePRK2 positively regulates pollen germination and tube growth and is involved in transducing responses to extracellular growth-promoting signals. © 2008 American Society of Plant Biologists. Fil:Wengier, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Muschietti, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320889_v148_n3_p1368_Zhang http://hdl.handle.net/20.500.12110/paper_00320889_v148_n3_p1368_Zhang
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	hybrid protein primer DNA protein kinase article enzymology germination growth, development and aging metabolism molecular genetics nucleotide sequence physiology pollen polymerase chain reaction signal transduction tomato transgenic plant Base Sequence DNA Primers Germination Lycopersicon esculentum Molecular Sequence Data Plants, Genetically Modified Pollen Polymerase Chain Reaction Protein Kinases Recombinant Fusion Proteins Signal Transduction Lycopersicon esculentum Magnoliophyta Solanum
spellingShingle	hybrid protein primer DNA protein kinase article enzymology germination growth, development and aging metabolism molecular genetics nucleotide sequence physiology pollen polymerase chain reaction signal transduction tomato transgenic plant Base Sequence DNA Primers Germination Lycopersicon esculentum Molecular Sequence Data Plants, Genetically Modified Pollen Polymerase Chain Reaction Protein Kinases Recombinant Fusion Proteins Signal Transduction Lycopersicon esculentum Magnoliophyta Solanum Wengier, Diego Leonardo Muschietti, Jorge P. The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth
topic_facet	hybrid protein primer DNA protein kinase article enzymology germination growth, development and aging metabolism molecular genetics nucleotide sequence physiology pollen polymerase chain reaction signal transduction tomato transgenic plant Base Sequence DNA Primers Germination Lycopersicon esculentum Molecular Sequence Data Plants, Genetically Modified Pollen Polymerase Chain Reaction Protein Kinases Recombinant Fusion Proteins Signal Transduction Lycopersicon esculentum Magnoliophyta Solanum
description	In flowering plants, the process of pollen germination and tube growth is required for successful fertilization. A pollen receptor kinase from tomato (Solanum lycopersicum), LePRK2, has been implicated in signaling during pollen germination and tube growth as well as in mediating pollen (tube)-pistil communication. Here we show that reduced expression of LePRK2 affects four aspects of pollen germination and tube growth. First, the percentage of pollen that germinates is reduced, and the time window for competence to germinate is also shorter. Second, the pollen tube growth rate is reduced both in vitro and in the pistil. Third, tip-localized superoxide production by pollen tubes cannot be increased by exogenous calcium ions. Fourth, pollen tubes have defects in responses to style extract component (STIL), an extracellular growth-promoting signal from the pistil. Pollen tubes transiently overexpressing LePRK2-fluorescent protein fusions had slightly wider tips, whereas pollen tubes coexpressing LePRK2 and its cytoplasmic partner protein KPP (a Rop-GEF) had much wider tips. Together these results show that LePRK2 positively regulates pollen germination and tube growth and is involved in transducing responses to extracellular growth-promoting signals. © 2008 American Society of Plant Biologists.
author	Wengier, Diego Leonardo Muschietti, Jorge P.
author_facet	Wengier, Diego Leonardo Muschietti, Jorge P.
author_sort	Wengier, Diego Leonardo
title	The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth
title_short	The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth
title_full	The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth
title_fullStr	The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth
title_full_unstemmed	The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth
title_sort	pollen receptor kinase leprk2 mediates growth-promoting signals and positively regulates pollen germination and tube growth
publishDate	2008
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320889_v148_n3_p1368_Zhang http://hdl.handle.net/20.500.12110/paper_00320889_v148_n3_p1368_Zhang
work_keys_str_mv	AT wengierdiegoleonardo thepollenreceptorkinaseleprk2mediatesgrowthpromotingsignalsandpositivelyregulatespollengerminationandtubegrowth AT muschiettijorgep thepollenreceptorkinaseleprk2mediatesgrowthpromotingsignalsandpositivelyregulatespollengerminationandtubegrowth AT wengierdiegoleonardo pollenreceptorkinaseleprk2mediatesgrowthpromotingsignalsandpositivelyregulatespollengerminationandtubegrowth AT muschiettijorgep pollenreceptorkinaseleprk2mediatesgrowthpromotingsignalsandpositivelyregulatespollengerminationandtubegrowth
_version_	1768546291237781504

The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth

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