Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells

Brassinosteroids (BRs) are involved in numerous physiological processes associated with plant development and especially with cell expansion. Here we report that two BRs, 28-homobrassinolide (HBL) and its direct precursor 28-homocastasterone (HCS), promote cell expansion of Arabidopsis thaliana susp...

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Autor principal:	Ramirez, Javier Alberto
Publicado:	2005
Materias:	Arabidopsis thaliana Brassinosteroid Ion currents Plasma membrane Proton pump Suspension cells anion ion channel proton pump steroid Arabidopsis article cell membrane cytology metabolism physiology Anions Cell Membrane Ion Channels Proton Pumps Steroids
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320781_v46_n9_p1494_Zhang http://hdl.handle.net/20.500.12110/paper_00320781_v46_n9_p1494_Zhang
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00320781_v46_n9_p1494_Zhang
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spelling	paper:paper_00320781_v46_n9_p1494_Zhang2023-06-08T14:59:54Z Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells Ramirez, Javier Alberto Arabidopsis thaliana Brassinosteroid Ion currents Plasma membrane Proton pump Suspension cells anion ion channel proton pump steroid Arabidopsis article cell membrane cytology metabolism physiology Anions Arabidopsis Cell Membrane Ion Channels Proton Pumps Steroids Arabidopsis Arabidopsis thaliana Brassinosteroids (BRs) are involved in numerous physiological processes associated with plant development and especially with cell expansion. Here we report that two BRs, 28-homobrassinolide (HBL) and its direct precursor 28-homocastasterone (HCS), promote cell expansion of Arabidopsis thaliana suspension cells. We also show that cell expansions induced by HBL and HCS are correlated with the amplitude of the plasma membrane hyperpolarization they elicited. HBL, which promoted the larger cell expansion, also provoked the larger hyperpolarization. We observed that membrane hyperpolarization and cell expansion were partially inhibited by the proton pump inhibitor erythrosin B, suggesting that proton pumps were not the only ion transport system modulated by the two BRs. We used a voltage clamp approach in order to find the other ion transport systems involved in the PM hyperpolarization elicited by HBL and HCS. Interestingly, while anion currents were inhibited by both HBL and HCS, outward rectifying K+ currents were increased by HBL but inhibited by HCS. The different electrophysiological behavior shown by HBL and HCS indicates that small changes in the BR skeleton might be responsible for changes in bioactivity. © 2005 JSPP. Fil:Ramirez, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320781_v46_n9_p1494_Zhang http://hdl.handle.net/20.500.12110/paper_00320781_v46_n9_p1494_Zhang
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Arabidopsis thaliana Brassinosteroid Ion currents Plasma membrane Proton pump Suspension cells anion ion channel proton pump steroid Arabidopsis article cell membrane cytology metabolism physiology Anions Arabidopsis Cell Membrane Ion Channels Proton Pumps Steroids Arabidopsis Arabidopsis thaliana
spellingShingle	Arabidopsis thaliana Brassinosteroid Ion currents Plasma membrane Proton pump Suspension cells anion ion channel proton pump steroid Arabidopsis article cell membrane cytology metabolism physiology Anions Arabidopsis Cell Membrane Ion Channels Proton Pumps Steroids Arabidopsis Arabidopsis thaliana Ramirez, Javier Alberto Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells
topic_facet	Arabidopsis thaliana Brassinosteroid Ion currents Plasma membrane Proton pump Suspension cells anion ion channel proton pump steroid Arabidopsis article cell membrane cytology metabolism physiology Anions Arabidopsis Cell Membrane Ion Channels Proton Pumps Steroids Arabidopsis Arabidopsis thaliana
description	Brassinosteroids (BRs) are involved in numerous physiological processes associated with plant development and especially with cell expansion. Here we report that two BRs, 28-homobrassinolide (HBL) and its direct precursor 28-homocastasterone (HCS), promote cell expansion of Arabidopsis thaliana suspension cells. We also show that cell expansions induced by HBL and HCS are correlated with the amplitude of the plasma membrane hyperpolarization they elicited. HBL, which promoted the larger cell expansion, also provoked the larger hyperpolarization. We observed that membrane hyperpolarization and cell expansion were partially inhibited by the proton pump inhibitor erythrosin B, suggesting that proton pumps were not the only ion transport system modulated by the two BRs. We used a voltage clamp approach in order to find the other ion transport systems involved in the PM hyperpolarization elicited by HBL and HCS. Interestingly, while anion currents were inhibited by both HBL and HCS, outward rectifying K+ currents were increased by HBL but inhibited by HCS. The different electrophysiological behavior shown by HBL and HCS indicates that small changes in the BR skeleton might be responsible for changes in bioactivity. © 2005 JSPP.
author	Ramirez, Javier Alberto
author_facet	Ramirez, Javier Alberto
author_sort	Ramirez, Javier Alberto
title	Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells
title_short	Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells
title_full	Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells
title_fullStr	Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells
title_full_unstemmed	Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells
title_sort	brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of arabidopsis thaliana cells
publishDate	2005
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320781_v46_n9_p1494_Zhang http://hdl.handle.net/20.500.12110/paper_00320781_v46_n9_p1494_Zhang
work_keys_str_mv	AT ramirezjavieralberto brassinosteroidsregulateplasmamembraneanionchannelsinadditiontoprotonpumpsduringexpansionofarabidopsisthalianacells
_version_	1768545499847065600

Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells

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