POLAR-guided signalling complex assembly and localization drive asymmetric cell division

Stomatal cell lineage is an archetypal example of asymmetric cell division (ACD), which is necessary for plant survival 1–4 . In Arabidopsis thaliana, the GLYCOGEN SYNTHASE KINASE3 (GSK3)/SHAGGY-like kinase BRASSINOSTEROID INSENSITIVE 2 (BIN2) phosphorylates both the mitogen-activated protein kinase...

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Detalles Bibliográficos
Publicado: 2018
Materias:
BIN2 protein
glycogen synthase kinase 3
mitogen activated protein kinase
plant protein
POLAR protein
protein kinase
SPEECHLESS protein
transcription factor
unclassified drug
Arabidopsis thaliana
asymmetric cell division
cell fate
cell lineage
cell nucleus
cellular distribution
complex formation
controlled study
cytosol
enzyme specificity
Letter
MAPK signaling
nonhuman
plant stoma
polarization
priority journal
protein expression
protein phosphorylation
signal transduction
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00280836_v563_n7732_p574_Houbaert
http://hdl.handle.net/20.500.12110/paper_00280836_v563_n7732_p574_Houbaert
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00280836_v563_n7732_p574_Houbaert
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spelling paper:paper_00280836_v563_n7732_p574_Houbaert2023-06-08T14:54:48Z POLAR-guided signalling complex assembly and localization drive asymmetric cell division BIN2 protein glycogen synthase kinase 3 mitogen activated protein kinase plant protein POLAR protein protein kinase SPEECHLESS protein transcription factor unclassified drug Arabidopsis thaliana asymmetric cell division cell fate cell lineage cell nucleus cellular distribution complex formation controlled study cytosol enzyme specificity Letter MAPK signaling nonhuman plant stoma polarization priority journal protein expression protein phosphorylation signal transduction Stomatal cell lineage is an archetypal example of asymmetric cell division (ACD), which is necessary for plant survival 1–4 . In Arabidopsis thaliana, the GLYCOGEN SYNTHASE KINASE3 (GSK3)/SHAGGY-like kinase BRASSINOSTEROID INSENSITIVE 2 (BIN2) phosphorylates both the mitogen-activated protein kinase (MAPK) signalling module 5,6 and its downstream target, the transcription factor SPEECHLESS (SPCH) 7 , to promote and restrict ACDs, respectively, in the same stomatal lineage cell. However, the mechanisms that balance these mutually exclusive activities remain unclear. Here we identify the plant-specific protein POLAR as a stomatal lineage scaffold for a subset of GSK3-like kinases that confines them to the cytosol and subsequently transiently polarizes them within the cell, together with BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE (BASL), before ACD. As a result, MAPK signalling is attenuated, enabling SPCH to drive ACD in the nucleus. Moreover, POLAR turnover requires phosphorylation on specific residues, mediated by GSK3. Our study reveals a mechanism by which the scaffolding protein POLAR ensures GSK3 substrate specificity, and could serve as a paradigm for understanding regulation of GSK3 in plants. © 2018, Springer Nature Limited. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00280836_v563_n7732_p574_Houbaert http://hdl.handle.net/20.500.12110/paper_00280836_v563_n7732_p574_Houbaert
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic BIN2 protein
glycogen synthase kinase 3
mitogen activated protein kinase
plant protein
POLAR protein
protein kinase
SPEECHLESS protein
transcription factor
unclassified drug
Arabidopsis thaliana
asymmetric cell division
cell fate
cell lineage
cell nucleus
cellular distribution
complex formation
controlled study
cytosol
enzyme specificity
Letter
MAPK signaling
nonhuman
plant stoma
polarization
priority journal
protein expression
protein phosphorylation
signal transduction
spellingShingle BIN2 protein
glycogen synthase kinase 3
mitogen activated protein kinase
plant protein
POLAR protein
protein kinase
SPEECHLESS protein
transcription factor
unclassified drug
Arabidopsis thaliana
asymmetric cell division
cell fate
cell lineage
cell nucleus
cellular distribution
complex formation
controlled study
cytosol
enzyme specificity
Letter
MAPK signaling
nonhuman
plant stoma
polarization
priority journal
protein expression
protein phosphorylation
signal transduction
POLAR-guided signalling complex assembly and localization drive asymmetric cell division
topic_facet BIN2 protein
glycogen synthase kinase 3
mitogen activated protein kinase
plant protein
POLAR protein
protein kinase
SPEECHLESS protein
transcription factor
unclassified drug
Arabidopsis thaliana
asymmetric cell division
cell fate
cell lineage
cell nucleus
cellular distribution
complex formation
controlled study
cytosol
enzyme specificity
Letter
MAPK signaling
nonhuman
plant stoma
polarization
priority journal
protein expression
protein phosphorylation
signal transduction
description Stomatal cell lineage is an archetypal example of asymmetric cell division (ACD), which is necessary for plant survival 1–4 . In Arabidopsis thaliana, the GLYCOGEN SYNTHASE KINASE3 (GSK3)/SHAGGY-like kinase BRASSINOSTEROID INSENSITIVE 2 (BIN2) phosphorylates both the mitogen-activated protein kinase (MAPK) signalling module 5,6 and its downstream target, the transcription factor SPEECHLESS (SPCH) 7 , to promote and restrict ACDs, respectively, in the same stomatal lineage cell. However, the mechanisms that balance these mutually exclusive activities remain unclear. Here we identify the plant-specific protein POLAR as a stomatal lineage scaffold for a subset of GSK3-like kinases that confines them to the cytosol and subsequently transiently polarizes them within the cell, together with BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE (BASL), before ACD. As a result, MAPK signalling is attenuated, enabling SPCH to drive ACD in the nucleus. Moreover, POLAR turnover requires phosphorylation on specific residues, mediated by GSK3. Our study reveals a mechanism by which the scaffolding protein POLAR ensures GSK3 substrate specificity, and could serve as a paradigm for understanding regulation of GSK3 in plants. © 2018, Springer Nature Limited.
title POLAR-guided signalling complex assembly and localization drive asymmetric cell division
title_short POLAR-guided signalling complex assembly and localization drive asymmetric cell division
title_full POLAR-guided signalling complex assembly and localization drive asymmetric cell division
title_fullStr POLAR-guided signalling complex assembly and localization drive asymmetric cell division
title_full_unstemmed POLAR-guided signalling complex assembly and localization drive asymmetric cell division
title_sort polar-guided signalling complex assembly and localization drive asymmetric cell division
publishDate 2018
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00280836_v563_n7732_p574_Houbaert
http://hdl.handle.net/20.500.12110/paper_00280836_v563_n7732_p574_Houbaert
_version_ 1768544990876663808

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