Leptin activates anorexigenic POMC neurons through a neural network in the arcuate nucleus

The administration of leptin to leptin-deficient humans, and the analogous Lepob/Lepob mice, effectively reduces hyperphagia and obesity. But common obesity is associated with elevated leptin, which suggests that obese humans are resistant to this adipocyte hormone. In addition to regulating long-te...

Descripción completa

Detalles Bibliográficos
Publicado: 2001
Materias:
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00280836_v411_n6836_p480_Cowley
http://hdl.handle.net/20.500.12110/paper_00280836_v411_n6836_p480_Cowley
Aporte de:
id paper:paper_00280836_v411_n6836_p480_Cowley
record_format dspace
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Electrophysiology
Hormones
Metabolism
Proteins
Neurons
Neural networks
4 aminobutyric acid
cation channel
green fluorescent protein
leptin
neuropeptide Y
proopiomelanocortin
action potential
animal cell
animal experiment
arcuate nucleus
article
controlled study
depolarization
embryo
gene expression
gene targeting
male
mouse
nerve cell
nerve cell network
nonhuman
priority journal
regulatory mechanism
transgenic mouse
Action Potentials
Animals
Animals, Genetically Modified
Anorexia
Arcuate Nucleus
Electrophysiology
Evoked Potentials
gamma-Aminobutyric Acid
Green Fluorescent Proteins
Leptin
Luminescent Proteins
Male
Mice
Mice, Inbred C57BL
Nerve Net
Neural Inhibition
Neurons
Neuropeptide Y
Pro-Opiomelanocortin
Animalia
Mus musculus
spellingShingle Electrophysiology
Hormones
Metabolism
Proteins
Neurons
Neural networks
4 aminobutyric acid
cation channel
green fluorescent protein
leptin
neuropeptide Y
proopiomelanocortin
action potential
animal cell
animal experiment
arcuate nucleus
article
controlled study
depolarization
embryo
gene expression
gene targeting
male
mouse
nerve cell
nerve cell network
nonhuman
priority journal
regulatory mechanism
transgenic mouse
Action Potentials
Animals
Animals, Genetically Modified
Anorexia
Arcuate Nucleus
Electrophysiology
Evoked Potentials
gamma-Aminobutyric Acid
Green Fluorescent Proteins
Leptin
Luminescent Proteins
Male
Mice
Mice, Inbred C57BL
Nerve Net
Neural Inhibition
Neurons
Neuropeptide Y
Pro-Opiomelanocortin
Animalia
Mus musculus
Leptin activates anorexigenic POMC neurons through a neural network in the arcuate nucleus
topic_facet Electrophysiology
Hormones
Metabolism
Proteins
Neurons
Neural networks
4 aminobutyric acid
cation channel
green fluorescent protein
leptin
neuropeptide Y
proopiomelanocortin
action potential
animal cell
animal experiment
arcuate nucleus
article
controlled study
depolarization
embryo
gene expression
gene targeting
male
mouse
nerve cell
nerve cell network
nonhuman
priority journal
regulatory mechanism
transgenic mouse
Action Potentials
Animals
Animals, Genetically Modified
Anorexia
Arcuate Nucleus
Electrophysiology
Evoked Potentials
gamma-Aminobutyric Acid
Green Fluorescent Proteins
Leptin
Luminescent Proteins
Male
Mice
Mice, Inbred C57BL
Nerve Net
Neural Inhibition
Neurons
Neuropeptide Y
Pro-Opiomelanocortin
Animalia
Mus musculus
description The administration of leptin to leptin-deficient humans, and the analogous Lepob/Lepob mice, effectively reduces hyperphagia and obesity. But common obesity is associated with elevated leptin, which suggests that obese humans are resistant to this adipocyte hormone. In addition to regulating long-term energy balance, leptin also rapidly affects neuronal activity. Proopiomelanocortin (POMC) and neuropeptide-Y types of neurons in the arcuate nucleus of the hypothalamus are both principal sites of leptin receptor expression and the source of potent neuropeptide modulators, melanocortins and neuropeptide Y, which exert opposing effects on feeding and metabolism. These neurons are therefore ideal for characterizing leptin action and the mechanism of leptin resistance; however, their diffuse distribution makes them difficult to study. Here we report electrophysiological recordings on POMC neurons, which we identified by targeted expression of green fluorescent protein in transgenic mice. Leptin increases the frequency of action potentials in the anorexigenic POMC neurons by two mechanisms: depolarization through a nonspecific cation channel; and reduced inhibition by local orexigenic neuropeptide-Y/GABA (γ-aminobutyric acid) neurons. Furthermore, we show that melanocortin peptides have an autoinhibitory effect on this circuit. On the basis of our results, we propose an integrated model of leptin action and neuronal architecture in the arcuate nucleus of the hypothalamus.
title Leptin activates anorexigenic POMC neurons through a neural network in the arcuate nucleus
title_short Leptin activates anorexigenic POMC neurons through a neural network in the arcuate nucleus
title_full Leptin activates anorexigenic POMC neurons through a neural network in the arcuate nucleus
title_fullStr Leptin activates anorexigenic POMC neurons through a neural network in the arcuate nucleus
title_full_unstemmed Leptin activates anorexigenic POMC neurons through a neural network in the arcuate nucleus
title_sort leptin activates anorexigenic pomc neurons through a neural network in the arcuate nucleus
publishDate 2001
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00280836_v411_n6836_p480_Cowley
http://hdl.handle.net/20.500.12110/paper_00280836_v411_n6836_p480_Cowley
bdutipo_str Repositorios
_version_ 1764820558689599490