Expression and biochemical characterisation of recombinant AceA, a bacterial α-mannosyltransferase

Biosynthesis of repeat-unit polysaccharides and N-linked glycans proceeds by sequential transfer of sugars from the appropriate sugar donor to an activated lipid carrier. The transfer of each sugar is catalysed by a specific glycosyltransferase. The molecular basis of the specificity of sugar additi...

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Detalles Bibliográficos
Autores principales: Geremía, Roberto Alejandro, Ferreiro, Diego U., Ielpi, Luis
Publicado: 1999
Materias:
Acetan
Acetobacter xylinum
Bacterial polysaccharide
Biosynthesis
Glycosyltransferase
bacterial enzyme
cellobiose
guanosine diphosphate mannose
mannose
mannosyltransferase
recombinant enzyme
acetobacter
article
controlled study
enzyme activity
escherichia coli
expression vector
gene overexpression
molecular cloning
nonhuman
open reading frame
priority journal
structure activity relation
Amino Acid Sequence
Base Sequence
Carbohydrate Sequence
Cloning, Molecular
DNA, Recombinant
Mannosyltransferases
Mutagenesis, Site-Directed
Open Reading Frames
Substrate Specificity
Acetobacter
Bacteria (microorganisms)
Escherichia coli
Gluconacetobacter xylinus
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00268925_v261_n6_p933_Geremia
http://hdl.handle.net/20.500.12110/paper_00268925_v261_n6_p933_Geremia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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