Cultured peripheral blood mononuclear leukocytes from anorexia nervosa patients are refractory to visible light
Cultured human peripheral blood mononuclear leukocytes [PBML] from patients with anorexia nervosa [AN] did not respond to light stimulation as PBML of normal controls [NC] did. During winter, visible light increased [3H]thymidine incorporation into DNA of NC-PBML stimulated with phytohemagglutinin [...
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1995
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v57_n6_p559_Finocchiaro http://hdl.handle.net/20.500.12110/paper_00243205_v57_n6_p559_Finocchiaro |
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paper:paper_00243205_v57_n6_p559_Finocchiaro2023-06-08T14:52:01Z Cultured peripheral blood mononuclear leukocytes from anorexia nervosa patients are refractory to visible light Finocchiaro, Liliana María Elena Glikin, Gerardo Claudio anorexia nervosa melatonin peripheral blood mononuclear leukocytes serotonin visible light 5 hydroxyindoleacetic acid iodine 125 melatonin phytohemagglutinin serotonin adolescent adult anorexia nervosa article blood cell culture circadian rhythm clinical article controlled study dna synthesis drug binding female human human cell leukocyte monoamine metabolism mononuclear cell photostimulation serotonin metabolism Adolescent Adult Anorexia Nervosa Cells, Cultured DNA Female Human Hydroxyindoleacetic Acid Leukocytes, Mononuclear Light Melatonin Opsin RNA, Messenger Seasons Serotonin Support, Non-U.S. Gov't Cultured human peripheral blood mononuclear leukocytes [PBML] from patients with anorexia nervosa [AN] did not respond to light stimulation as PBML of normal controls [NC] did. During winter, visible light increased [3H]thymidine incorporation into DNA of NC-PBML stimulated with phytohemagglutinin [PHA]. This effect was enhanced by 10-7 M melatonin. PHA-stimulated DNA synthesis of PBML from AN patients failed to respond to photic stimulation during winter and their proliferative response to melatonin was significantly blunted. In vitro photic stimulation of NC-PBML reduced melatonin while increasing both serotonin and 5-hydroxyindole 3-acetic acid [HIAA] production in both basal and PHA-stimulated conditions. In contrast AN-PBML, that in darkness enhanced the oxidative deamination of serotonin into HIAA more than NC-PBML, did not switch their indole metabolism in response to light. Light did not inhibit the binding of both melatonin and serotonin to AN-PBML as occurred in NC-PBML. The present data suggest that AN-PBML do not respond to light in vitro, because of a failure in the regulation of serotonin and melatonin metabolism. © 1995. Fil:Finocchiaro, L.M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Glikin, G.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1995 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v57_n6_p559_Finocchiaro http://hdl.handle.net/20.500.12110/paper_00243205_v57_n6_p559_Finocchiaro |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
anorexia nervosa melatonin peripheral blood mononuclear leukocytes serotonin visible light 5 hydroxyindoleacetic acid iodine 125 melatonin phytohemagglutinin serotonin adolescent adult anorexia nervosa article blood cell culture circadian rhythm clinical article controlled study dna synthesis drug binding female human human cell leukocyte monoamine metabolism mononuclear cell photostimulation serotonin metabolism Adolescent Adult Anorexia Nervosa Cells, Cultured DNA Female Human Hydroxyindoleacetic Acid Leukocytes, Mononuclear Light Melatonin Opsin RNA, Messenger Seasons Serotonin Support, Non-U.S. Gov't |
| spellingShingle |
anorexia nervosa melatonin peripheral blood mononuclear leukocytes serotonin visible light 5 hydroxyindoleacetic acid iodine 125 melatonin phytohemagglutinin serotonin adolescent adult anorexia nervosa article blood cell culture circadian rhythm clinical article controlled study dna synthesis drug binding female human human cell leukocyte monoamine metabolism mononuclear cell photostimulation serotonin metabolism Adolescent Adult Anorexia Nervosa Cells, Cultured DNA Female Human Hydroxyindoleacetic Acid Leukocytes, Mononuclear Light Melatonin Opsin RNA, Messenger Seasons Serotonin Support, Non-U.S. Gov't Finocchiaro, Liliana María Elena Glikin, Gerardo Claudio Cultured peripheral blood mononuclear leukocytes from anorexia nervosa patients are refractory to visible light |
| topic_facet |
anorexia nervosa melatonin peripheral blood mononuclear leukocytes serotonin visible light 5 hydroxyindoleacetic acid iodine 125 melatonin phytohemagglutinin serotonin adolescent adult anorexia nervosa article blood cell culture circadian rhythm clinical article controlled study dna synthesis drug binding female human human cell leukocyte monoamine metabolism mononuclear cell photostimulation serotonin metabolism Adolescent Adult Anorexia Nervosa Cells, Cultured DNA Female Human Hydroxyindoleacetic Acid Leukocytes, Mononuclear Light Melatonin Opsin RNA, Messenger Seasons Serotonin Support, Non-U.S. Gov't |
| description |
Cultured human peripheral blood mononuclear leukocytes [PBML] from patients with anorexia nervosa [AN] did not respond to light stimulation as PBML of normal controls [NC] did. During winter, visible light increased [3H]thymidine incorporation into DNA of NC-PBML stimulated with phytohemagglutinin [PHA]. This effect was enhanced by 10-7 M melatonin. PHA-stimulated DNA synthesis of PBML from AN patients failed to respond to photic stimulation during winter and their proliferative response to melatonin was significantly blunted. In vitro photic stimulation of NC-PBML reduced melatonin while increasing both serotonin and 5-hydroxyindole 3-acetic acid [HIAA] production in both basal and PHA-stimulated conditions. In contrast AN-PBML, that in darkness enhanced the oxidative deamination of serotonin into HIAA more than NC-PBML, did not switch their indole metabolism in response to light. Light did not inhibit the binding of both melatonin and serotonin to AN-PBML as occurred in NC-PBML. The present data suggest that AN-PBML do not respond to light in vitro, because of a failure in the regulation of serotonin and melatonin metabolism. © 1995. |
| author |
Finocchiaro, Liliana María Elena Glikin, Gerardo Claudio |
| author_facet |
Finocchiaro, Liliana María Elena Glikin, Gerardo Claudio |
| author_sort |
Finocchiaro, Liliana María Elena |
| title |
Cultured peripheral blood mononuclear leukocytes from anorexia nervosa patients are refractory to visible light |
| title_short |
Cultured peripheral blood mononuclear leukocytes from anorexia nervosa patients are refractory to visible light |
| title_full |
Cultured peripheral blood mononuclear leukocytes from anorexia nervosa patients are refractory to visible light |
| title_fullStr |
Cultured peripheral blood mononuclear leukocytes from anorexia nervosa patients are refractory to visible light |
| title_full_unstemmed |
Cultured peripheral blood mononuclear leukocytes from anorexia nervosa patients are refractory to visible light |
| title_sort |
cultured peripheral blood mononuclear leukocytes from anorexia nervosa patients are refractory to visible light |
| publishDate |
1995 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v57_n6_p559_Finocchiaro http://hdl.handle.net/20.500.12110/paper_00243205_v57_n6_p559_Finocchiaro |
| work_keys_str_mv |
AT finocchiarolilianamariaelena culturedperipheralbloodmononuclearleukocytesfromanorexianervosapatientsarerefractorytovisiblelight AT glikingerardoclaudio culturedperipheralbloodmononuclearleukocytesfromanorexianervosapatientsarerefractorytovisiblelight |
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1768542927837986816 |