Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals
Samples containing as precursors either 18-hydroxycorticosterone (18-OH-B) in its M form, or this converted to less polar forms at pH 2 (ACM), or M or ACM enclosed in liposomes from adrenal lipids were incubated at pH 7.4, 4.8 or 3.3 in the presence or absence of quartered rat adrenals for 1 and 2 h...
Guardado en:
Autores principales: | , , |
---|---|
Publicado: |
1985
|
Materias: | |
Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v22_n5_p665_Cozza http://hdl.handle.net/20.500.12110/paper_00224731_v22_n5_p665_Cozza |
Aporte de: |
id |
paper:paper_00224731_v22_n5_p665_Cozza |
---|---|
record_format |
dspace |
spelling |
paper:paper_00224731_v22_n5_p665_Cozza2023-06-08T14:51:01Z Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals Cozza, Eduardo Néstor Burton, Gerardo Ceballos, Nora Raquel 18 deoxyaldosterone 18 hydroxycorticosterone aldosterone radioisotope unclassified drug adrenal gland animal cell article endocrine system nonhuman priority journal rat 18-Hydroxycorticosterone Adrenal Glands Aldosterone Animals Corticosterone Hydrogen-Ion Concentration Liposomes Magnetic Resonance Spectroscopy Male Rats Animalia Samples containing as precursors either 18-hydroxycorticosterone (18-OH-B) in its M form, or this converted to less polar forms at pH 2 (ACM), or M or ACM enclosed in liposomes from adrenal lipids were incubated at pH 7.4, 4.8 or 3.3 in the presence or absence of quartered rat adrenals for 1 and 2 h. Optimal (10%) yields of aldosterone were obtained when (a) ACM was incubated at pH 4.8 and (b) M enclosed in liposomes was suspended in buffer and shaken without enzyme at pH 3.3. When conditions (a) were supplemented with malate and NADP, 16% of ACM was converted to aldosterone. ACM contained 80% of a fraction which, according to 13C NMR spectroscopy, was identical to 18-deoxyaldosterone (18-DAL). Experiments in which radioactivity from corticosterone (B) or M was trapped by radioinert M or 18-DAL disclosed a pathway comprising sequentially B, 18-OH-B, 18-DAL and aldosterone, and the combined evidence of this work, an enzymatic hydroxylation of 18-DAL to aldosterone. © 1985. Fil:Cozza, E.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Burton, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceballos, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1985 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v22_n5_p665_Cozza http://hdl.handle.net/20.500.12110/paper_00224731_v22_n5_p665_Cozza |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
18 deoxyaldosterone 18 hydroxycorticosterone aldosterone radioisotope unclassified drug adrenal gland animal cell article endocrine system nonhuman priority journal rat 18-Hydroxycorticosterone Adrenal Glands Aldosterone Animals Corticosterone Hydrogen-Ion Concentration Liposomes Magnetic Resonance Spectroscopy Male Rats Animalia |
spellingShingle |
18 deoxyaldosterone 18 hydroxycorticosterone aldosterone radioisotope unclassified drug adrenal gland animal cell article endocrine system nonhuman priority journal rat 18-Hydroxycorticosterone Adrenal Glands Aldosterone Animals Corticosterone Hydrogen-Ion Concentration Liposomes Magnetic Resonance Spectroscopy Male Rats Animalia Cozza, Eduardo Néstor Burton, Gerardo Ceballos, Nora Raquel Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals |
topic_facet |
18 deoxyaldosterone 18 hydroxycorticosterone aldosterone radioisotope unclassified drug adrenal gland animal cell article endocrine system nonhuman priority journal rat 18-Hydroxycorticosterone Adrenal Glands Aldosterone Animals Corticosterone Hydrogen-Ion Concentration Liposomes Magnetic Resonance Spectroscopy Male Rats Animalia |
description |
Samples containing as precursors either 18-hydroxycorticosterone (18-OH-B) in its M form, or this converted to less polar forms at pH 2 (ACM), or M or ACM enclosed in liposomes from adrenal lipids were incubated at pH 7.4, 4.8 or 3.3 in the presence or absence of quartered rat adrenals for 1 and 2 h. Optimal (10%) yields of aldosterone were obtained when (a) ACM was incubated at pH 4.8 and (b) M enclosed in liposomes was suspended in buffer and shaken without enzyme at pH 3.3. When conditions (a) were supplemented with malate and NADP, 16% of ACM was converted to aldosterone. ACM contained 80% of a fraction which, according to 13C NMR spectroscopy, was identical to 18-deoxyaldosterone (18-DAL). Experiments in which radioactivity from corticosterone (B) or M was trapped by radioinert M or 18-DAL disclosed a pathway comprising sequentially B, 18-OH-B, 18-DAL and aldosterone, and the combined evidence of this work, an enzymatic hydroxylation of 18-DAL to aldosterone. © 1985. |
author |
Cozza, Eduardo Néstor Burton, Gerardo Ceballos, Nora Raquel |
author_facet |
Cozza, Eduardo Néstor Burton, Gerardo Ceballos, Nora Raquel |
author_sort |
Cozza, Eduardo Néstor |
title |
Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals |
title_short |
Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals |
title_full |
Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals |
title_fullStr |
Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals |
title_full_unstemmed |
Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals |
title_sort |
eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals |
publishDate |
1985 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v22_n5_p665_Cozza http://hdl.handle.net/20.500.12110/paper_00224731_v22_n5_p665_Cozza |
work_keys_str_mv |
AT cozzaeduardonestor eighteendeoxyaldosteroneandotherlesspolarformsof18hydroxycorticosteroneasaldosteroneprecursorsinratadrenals AT burtongerardo eighteendeoxyaldosteroneandotherlesspolarformsof18hydroxycorticosteroneasaldosteroneprecursorsinratadrenals AT ceballosnoraraquel eighteendeoxyaldosteroneandotherlesspolarformsof18hydroxycorticosteroneasaldosteroneprecursorsinratadrenals |
_version_ |
1768542445297991680 |